Blood Lipid Pro le of Hypercholesterolemia Rattus norvegicus L. Fed with Sausages Containing Omega 3 and Omega 6 Fatty Acids

Abstract

The experiment was conducted to investigate blood lipid pro le of hypercholesterolemia Rattus norvegicus L. fed with sausages containing omega 3 and omega 6 fatty acids. Twenty four male Sprague dawley breed rats of two months old were used in this experiment and placed in individual cage. They were grouped into four groups consisted of six rats and fed with basal ration, and drinking water ad lib. The rats were given sausages with different level of omega 3 and omega 6 fatty acids with ratio of 1 : 5.29. The levels were 1.78 g/ rat/day (low level); 3.56 g/rat/day (medium level); and 7.11 g/rat/day (high level); and control. Blood was taken at the venous sinus retro-orbital of rats for the lipid pro le analysis (cholesterol, triglyceride, low density lipoprotein, and high density lipoprotein). The data of lipid pro le were analyzed statistically using analysis of variance and the differences between means were tested by Duncan’s new multiple range test. The feeding of sausages containing omega 3 and omega 6 in uenced signi cantly on the blood lipid pro le of rats (P<0.05). The blood of rats fed with sausages containing omega 3 and omega 6 fatty acids at the level of 7.11 g/rat/day (high level) had lower cholesterol 113.77±5.65 mg/dl, triglyceride 81.55±7.35 mg/dl, and low density lipoprotein 50.56±6.41 mg/dl, and had higher high density lipoprotein 64.51±3.43 mg/dl compared to control that contained cholesterol 218.54±6.51 mg/dl, triglyceride 142.71±9.27 mg/dl, and low density lipoprotein 100.41±5.97 mg/dl, and high density lipoprotein 20.98±2.36 mg/dl (P<0.05). In conclusion, the rats fed with sausages containing omega 3 and omega 6 fatty acids at low, medium, and high levels improve the blood pro le of rats in term of decrease of cholesterol, triglyceride, and low density lipoprotein and of increase of high density lipoprotein.