In Vitro Maturation Rate of Bligon Goat Oocytes Supplemented With Gonadotropin

Abstract

Research to determine in vitro maturation rate of Bligon Goat supplemented with gonadotrophin (human chorionic gonadotrophin = hCG) in maturation medium. Cumulus-oocyte complexes of Bligon Goat were in vitro matured for 24 h in TCM 199 supplemented with 10 IU and without hCG . Oocytes were then stained with 1% aceto orcein to examine changes in the con guration of chromosomes and nuclear membrane. Oocytes were considered mature when reached metaphase II (MII), characterized by rst polar body (PB1) extrusion and arrangement chromosome similar to rst metaphase stage I (M1). The results demonstrated that oocytes will ef ciently undergo IVM under hCG supplementation. hCG supplementation signi cantly increased proportion oocyte underwent maturation (69.1±1.471 versus 44.2±2.5 %) as also indicated by nucleasr maturation and MII. Also lower rate of oocytes degeneration were observed in the medium with hCG supplementation (2.1±0.3. versus 10.7±2.3 %). It could be concluded that gonadotrophin supplementation was effective to improve oocytes maturation in vitro and yielding more mature oocytes for future in vitro fertilization.