The Detailed Motility and Velocity Characteristics of Rams Spermatozoa as Assessed by Computer-Aided Semen Analysis

Abstract

The aim of the present study was to determine the effect of incubation time in TALP mediumonthedetailedmotilityandvelocityofspermatozoacharacteristics.Four rams(R9,R12, R13, R16) of proven fertility were used in this study. Semen was collected by electroejaculation. Rams semen was collected with 4 replicates, respectively to analyzed the motility and velocity characteristics by using the computer-aided semen analysis (CASA). Data were analyzed using SPSS software program. The results showed that percentage of motile, progressive and rapid were signi cantly decreased from 0 to 120 minutes incubation. In contrast, percentage of static was signi cantly increased from 21.6% to 71.3% at 0 to 120 minutes incubation. Ram 12 had the best motile with 76.3% at 0 minute incubation and 40.3% at 120 minutes incubation. Average path velocity (VAP), progressive velocity (VSL) and track speed (VCL) at 0 incubation were 91.2 μm/s, 72.2 μm/s and 134 μm/s afterward decreased to 49 μm/s, 40.8 um/s and 77.7 μm/s at 120 minutes incubation, respectively. In conclusion, rams and incubation time were in uenced the motility and velocity of spermatozoa.