Isoptericola sp. A10-1, Chitinase Producing Actinobacterium Isolated from Indonesian Tropical Shrimp Pond Waste Water

Abstract

The purpose of this study was to obtain superior chitinolytic bacteria capable of degrading chitin from the waste water of tropical shrimp pond. The bacterium characterization was conducted to yield high-activity chitinase isolates, while bacterium identi cation was carried out based on morphology, physiology, biochemistry and molecular. The 16SrRNA method was used to identify strain Isoptericola sp. A10-1, similarity (99%). This strain was able to produce of extracellular chitinase in media containing colloidal chitin a carbon source. Results of morphological identi cation, biochemical and molecular identi ed as Isoptericola sp. A10- 1 including the genus Isoptericola showed chitinase activity in medium containing chitin as a carbon source. Chitinolytic bacteria Isoptericola sp. A10-1 are able to degrade chitin speci cally into the monomer in the form of glucosamine. They are widely used in the elds of agriculture, food and health industries.