OBSERVATION ON BALI CATTLE SPERM QUALITY BEFORE AND AFTER CENTRIFUGATION X AND Y CHROMOSOME OF SEPARATION

Abstract

The existence of Bali cattle as an indigenous cattle supposed to be maintained
regarding to enhance national production of meat and stocks. Therefore, the bio-
reproduction technology is needed to asses and implement. In other view, the calf which was female or male could be initially determined by sperm separation techniques. The research is conducted to identify bull sperm quality of Bali cattle that subjected to the separation of X and Y chromosomes. Two bulls of UPTD Bali namely Karamba (live weight 604 kg) and Moltang (588 kg) were used as semen source. Collecting semen was embraced at 10.00 am. Microscopic evaluation was conducted by using integrated soft-ware of Sperm Vision (Mini TUB). Semen were diluted by Egg-yolk Tris-amino methane and mixed by albumen that contained glycerol 13 %.The centrifugation was completed at 1500 rpm and 5 minute. The data were: motility (%), progressive motility (%), concentration (billion/ml), immotile (%), hyperactive movement (%), linear and non-linear movement (%). Each sample were observed at four fields. The result showed that fresh semen per ejaculate of Karamba and Moltang as 4 and 8.5 ml with 3.495 billion/ml and 2.396 billion/ml of concentration, respectively. Meanwhile, the progressive motility was 68.00 and 67.38 %. In this condition, the linear and hyperactive of Karamba as of 63.20 % and 2.47 % were higher than those of Moltang (60.40 % and 1.60 %). After centrifugation, the motility of Karamba was decrease as 59.46 % at upper fraction and 67.61 % at lower fraction; in the mean time the motility of Moltang as 71.98 % (upper fraction) and 77.55 % (lower fraction). This lessen was followed by the decrease of viable spenn at before and after centrifugation. However, it was still on the range of minimum standard, hence, it can be processed in the further form of chilled straw or frozen straw.