RUMINAL FERMENTATION AND MICROBIAL NITROGEN SYNTHESIS IN BUFFALO FED FIBROUS FEEDS

Abstract

To investigate the ruminal fermentation status (N-NHL VFA, pH) and microbial
nitrogen synthesis (allantoin and uric acid), three mature rumen cannulated buffaloes and six mature non mmen cannulated buffaloes were used respectively. All animals were fed peanut haulm (PI-I), king grass (KG) and com stover (CS) ad libirum as sole feed. The data was analyzed using variance analysis and followed by Duncan’s multiple range test. Ruminal fermentation data showed that ruminal N-NH; concentration during 24 h for PH was higher (P<.01) than that of CS and KG, of l8.89, 8.62, 5.18 mg/100 ml, respectively. Total VFA concentration during 24 h was not different among feed types, of 61.72, 55.44, 48.98 mmol/l for KG, CS and PH respectively. Ruminal pH was not influenced by feed types. Parameters
collected on microbial nitrogen synthesis showed that allantoin and uric acid was not affected by feed types, on the other hand the total purine derivates of PH was higher (P<.05) than that of CS and KG, but there was not different between CS and KG. The results showed that purine derivates excretion has the tendency to be affected by DDMI or DOMR as showed by regression equation Y=5.53 + 3.55X (R’=0.30, P<.05) and Y=6.l3 + 5.5lX (R‘=0.3l, P<.05), respectively. The highest microbial nitrogen synthesis in the rumen was produced by PH followed by CS and KG.