Preliminary Study of Liquid Hydrocarbon Biodegradation By Indigineous Bacteria Isolated from Wonocolo Village, Bojonegoro District, East Java Province
Wahyu Wilopo(1*), Bramono Dwi Kusumo(2), Endah Retnaningrum(3)
(1) Universitas Gadjah Mada
(2) Universitas Gadjah Mada
(3) Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Aquatic environmental pollution due to petroleum waste can cause disruption to the environment and damaging of flora and fauna. It has been reported that petroleum contaminatin occurs in the Bengawan Solo river, East Java Province. Liquid hydrocarbon waste pollution can be remediate through various processes, one of them is biodegradation. Biodegradation a part of bioremediation, is the process by which organic substances are decomposed by microorganisms into simpler substances such as carbon dioxide, water and ammonia. Bioremediation has minor side effects compare to other methods because it’s more effective, efficient, economical and eco-friendly through biological process. This study aims to identify bacteria for liquid hydrocarbon degradation from the rivers in Wonocolo Village, Bojonegoro District and to determine maximum percentage of inoculum to produce the highest efficiency of liquid hydrocarbons degradation. Based on phenotypic characters, the selected bacteria was identified as a genus of Moraxella. Its bacterium with a concentration of 2 % can reduce hydrocarbons to a maximum of 0.67 % per hour at the exponential phase growth.
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Breed, R. S., Murray, E. G. D., and Smith, N. R. (1957) Bergey’s Manual of Determinative Bacteriology, Seventh Edition, United States of America, The Williams and Wilkins Company.
Hadioetomo, R. S. (1985) Basic Microbiology in Practice of Basic Laboratory Techniques and Procedures, Jakarta, PT Gramedia.
Jednak, T., Avdalovic, J., Milatic, S., Beskoski, L. S., Stankovic, D., Milic, J., Ilic, M., Beskoski, V., Cvijovic, G. G., and Vrvic, M. M. (2017) Transformation and synthesis of humic substances during bioremediation of petroleum hydrocarbons, International Biodeterioration and Biodegradation, Vol. 122, pp. 47-52.
Leahy, J.G., Colwell, R.R. (1990) Microbial Degradation of Hydrocarbon in the Environments, Microbiological Reviews, Vol. 54:3.
Leisinger, T. (1981) Microbial Degradation of Xenobiotic and Recalsitrant Coumpound, London, Academici Press.
Madigan, M. T., Martinko, J. M., Bender, K. S., Buckley, D. H., and Stahl, D. A. (2012) BrockBiology of Microorganisms, 13th Edition, San Fransisco, Benjamin Cummings.
Marzan, L. W., Sultana, T., Hasan, Md. M., Mina, S. A., Islam, Md. R., Rakibuzzaman, A. G. M., Khan, Md. I. H. (2017) Characteization of furnace oil bioremediation potential of hydrocarbonoclastic bacteria isolated from petroleum contaminated sites of the Sundarbans, Bangladesh, Journal of Genetic Engineering and Biotechnology, Vol.15, pp. 103-113.
Azubuike, C.C, Blaise Chikere, C.B., and Okpokwasili, G.C. (2016) Bioremediation techniques classification based on site of application: principles, advantages, limitations and prospects, World J MicrobiolBiotechnol, Vol. 32:180, 18p.
Varjani, S. J., and Upasani, V. N., 2017,Anew look on factors affecting microbial degradation of petroleum hydrocarbon pollutants, International Biodeterioration and Biodegradation, Vol.120, pp.71-83.
Wilopo, W., Sasaki, K., Hirajima, T., Yamanaka, T. (2008) Immobilization of arsenic and manganese in contaminated groundwater by permeable reactive barriers using zero valent iron and sheep manure, Materials transactions, Vol. 49:10, pp. 2265- 2274
DOI: https://doi.org/10.22146/jag.40862
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