ISOLASI DAN KARAKTERISASI BAKTERI PENDENITRIFIKASI YANG DIISOLASI DARI LUMPUR KAWASAN MANGROVE

https://doi.org/10.22146/jfs.8912

Triyanto Triyanto(1*), Alim Isnansetyo(2), Irfan D. Prijambada(3), Jaka Widada(4), Duranta D. Kembaren(5)

(1) Jurusan Perikanan, Fakultas Pertanian UGM
(2) Jurusan Perikanan, Fakultas Pertanian UGM
(3) Laboratorium Mikrobiologi Pertanian UGM
(4) Laboratorium Mikrobiologi Pertanian UGM
(5) Jurusan Perikanan, Fakultas Pertanian UGM
(*) Corresponding Author

Abstract


The aim of this research is to isolate denitrifying bacteria which have the highest activity to reduce nitrate. The sources of the denitrifying bacteria were mangrove sediment collected from Cilacap Regency, Central Java and Indramayu Regency, West Java. Basalt medium containing KNO3 as a source of nitrogen was used for isolating the denitrifying bacteria. Double layer agar was used for making anaerob condition. Fourty-one isolates were obtained at the first step of the isolation, 29 of them have nitrate reduction activity at a range of 0.77-95.62%. Three isolates, i.e. D19.2, DR2.1 and D27.3 having the highest activity were selected for further examination. The selected isolates were characterized and identified. Characterization includes colony and cell morphology, Gram staining, motility, spore staining and biochemical tests as catalase and oxidase. Identification was done by using profile matching to Bergey’s Manual of Determinative Bacteriology. The results indicate that isolate D19.2 and D27.3 have similarities to the characters of genus Listeria, whereas isolate DR2.1 has similarities to the characters of genus Propionibacterium. All of the selected isolates were able to grow in a medium having NaCl concentration at a range of 0.5-3.5% and pH range of 5-8. Observation of nitrate reduction ability of the isolates during five days incubation shows that isolate DR2.1 has the highest denitrifying activity. The selected isolates can be used as bioremediation agents for controlling nitrate pollution in brackish water pond.

Keywords


isolation, denitrifying bacteria, mangrove sediment

Full Text:

PDF


References

Bazylinski, D.A., and R.P. Blakemore. 1983. Denitrification and assimilatory nitrate reduction in Aquaspirillum magnetotacticum. Appl. Environ. Microbiol. 46:1118-1124.

Boyd, C.E., 1989. Water quality and management and aeration in shrimp farming. Auburn University, Alabama.

Burford, M.A., C.J. Jackson, and N.P. Preston, 2001. Reducing nitrogen waste from shrimp farming: an integrated approach. In: The new wave, proceeding of the special session on sustainable shrimp culture, Aquaculture 2001C. L. Browdy and J.E. Jory (Eds.). The WAS, Louisiana, USA. 35-43.

Burton, F.L. 1991. Wastewater engineering: treatment disposal reuse. 3rd ed. McGrawHill International. Singapore.

Cappuccino, J.G and N. Sherman. 2001. Microbiology: a laboratory manual. Benjamin/Cumming Publishing, California.

Funge-Smith, S.J. and M.R.P. Briggs, 1998. Nutrient budgets in intensive shrimp ponds: implications for sustainability. Aquaculture. 164: 117-133.

Greenberg, A.E., R.R. Trussell, and L.S. Clesceri. 1985. Standard method for the examination of water and wastewater. APHA, Washington.

Holt, G.J., N.R. Krieg, P.H.A. Sneath, J.T. Staley, and S.T. William. 2000. Bergey’s Manual for Determinative Bacteriology. 9th eds. William&Wilkins, Baltimore.

Lim, C. dan D.S. Suanta. 1993. Budidaya tambak udang intensif. PT. Dipasena Citra Darmaja, Lampung.

Moss, S.M., S.M. Arce, B.J. Argue, C.A. Otoshi, F.R.O. Calderon and A.G.J. Tacon. 2001. Greening of the blue revolution: efforts toward environmentally responsible shrimp culture. In: The new wave, proceeding of the special session on sustainable shrimp culture, Aquaculture 2001. C.L. Browdy & J.E. Jory (Eds.). The WAS. Louisiana. USA. 1-19.

Oremland, R.S., C. Umberger, C.W. Culbertson, and R.L. Smith. 1984. Denitrification in San Fransisco Bay intertidal sediments. Appl. Environ. Microbil. 47: 1106-1112.

Payne, W.J. 1981. Denitrification. Willey-Interscience. New York.

Rodina, A.G. 1972. Methods in Aquatic Microbiology. University Park Pers. Baltimore.

Schaperclause, W. 1992. Fish Diseases. Vol. 1. A.A. Balkema. Rotterdam.

Sorensen, J. 1978. Denitrification rates in a marine sediments as measured by the aacetylene inhibition technique. Appl. Environ. Microbiol. 36 : 139-143.

Stainer, R.Y, J.L. Ingraham, M.L. Wheelis, and P.R. Painter. 1986. The Microbial World. 5th ed. Prentice-Hall. London.

Watson, I. 2002. The effect of exposure to oxygen on diauxic lag of Pseudomonas denitrificanse. J. Undergraduate Research. 4(2): 30-34.

Wyban, J.A. and J.N. Sweeney. 1991. Intensive shrimp production technology. The Oceanic Institute. Honolulu. Hawaii.



DOI: https://doi.org/10.22146/jfs.8912

Article Metrics

Abstract views : 680 | views : 6506

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Journal of Fisheries Sciences



Jurnal Perikanan Universitas Gadjah Mada Indexed by:

 

 

View My Stats