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Keywords Aeromond Azolla sp. COD Cilala reservoir, limnological status Kappaphycus alvarezii Oreochromis niloticus Serratia marcescens PT-6 Streptococcus iniae Yogyakarta african catfish completely randomize design egg laying season green turtles growth identification laundry waste water maturation pgm gene sangalaki island seaweed water quality