Suppression of Sheath Blight of Rice with Antagonistic Bacteria

https://doi.org/10.22146/jpti.10073

Arif Wibowo(1*)

(1) Faculty of Agriculture Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Eleven bacterial isolates were isolated from wet rice field in Wates, Kulon Progo. The result of antagonism test on nutrient agar (NA) showed that only two isolates (WKP-4 and WKP-6) were antagonistic to Rhizoctonia solani. Determination on these two isolates showed that they were belong to the group of fluorescent pseudomonads. In the glass house trial, only WKP-6 suppressed the relative lesion height of sheath blight of rice variety IR 50.


Keywords


Rhizoctonia solani; sheath bight of rice; fluorescent pseudomonads

Full Text:

PDF


References

Ahn, S.W., R.C. dela Pena, B.L. Candole & T.W. Mew. 1986. A New Scale for Rice Sheath Blight (ShB) Disease Assessment. IRRN 11 (6): 17.

Arie, T., S. Namba, S. Yamashita, Y. Doi & T. Kijima. 1987. Biological Control of Fusarium Wilt of Botle Gourd by Mix- cropping with Welsh Onion or Chinese Chieve Inoculated with Pseudomonas gladioli. Ann. Pyhtopath. Soc. Japan 53: 531─539.

Defago, G., C.H. Berling, U. Burger, D. Haas, G. Kahr, C. keel, C. Voistard, P. Wirthner & B. Wurthrich. 1990. Suppression of Blackroot of Tobacco and Other Root Diseases by Strain of Pseudomonas fluorescens: Potential, Application and Mechanisms, p. 75─81. In Hornby, D. (ed.), Biological Control of Soil Borne Pathogens. CAB International. Wallingford.

Doutt, R.L. 1972. Biological Control: Parasites and Predators, p. 288─297. In Anonim, Pest and Strategies in the Future. National Academy of Science. Washington D.C.

Devi, T.V. & R.M. Vishi. 1989. Biological Control of Sheath Blight of Rice in India with Antagonistic Bacteria. Plant and Soil 119: 325─330.

Fernando, W.G.D., A.K. Watson & T.C. Paulitz. 1994. Phylloplane Pseudomonas spp. Enhance Disease Caused by Colletotrichum coccodes on Velvetleaf. Biological Control 4: 125─131.

Gamliel, A. & J. Katan. 1993. Suppression of Major and Minor Pathogens by Fluorescent Pseudomonads in Solarized and Non-Solarized Soils. Phytopathology 83: 68─75.

Harrison, L.A., L. Letendre, P. Kovacevich, A.Pierson & D.M. Weller. 1993. Purification of Antibiotic Effective against Gaeumannomyces graminis var. tritici Produced by a Biocontrol Agent Pseudomonas aeurofaciens. Soil Biology and Biochem. 25: 215─221.

Hebbar, K.P., A.G. Davey, J. Merin & P.J. Dart. 1992. Rhizobacteria of Maize Antagonistic to Fusarium monilformae, a Soil Borne Fungal Pathogen: Colonization of Rhizosphere and Roots. Soil Biology and Biochem. 24: 989─997.

Homa, Y. & T. Suzui. 1989. Role of Antibiotic Production in Suppression of Radish Damping-off by Seed Bacterization with Pseudomonas cepacia. Ann. Phytopath. Soc. Japan 55: 643─652.

Jutono. 1980. Pedoman Praktikum Mikrobiologi Umum untuk Perguruan Tinggi. Departemen Mikrobiologi Fakultas Pertanian Universitas Gadjah Mada, Yogyakarta. 181 p.

Leifert, C., H. Li, S. Chidburee, S. Hampson, S. Workman, D. Sigee, H.A.S. Epton & A. Harbour. 1995. Antibiotic Production and Biocontrol Activity by Bacillus subtilis CL27 and Bacillus pumilus CL45. J. Appi. Bacteriol. 78: 97─108.

Mazolla, M. 1999. Transformation of Soil Microbial Community Structure and Rhizoctonia- Suppressive Potential in Response to Apple Roots. Phytopathology 89: 920─927.

Mazolla, M., D.K. Fujimoto, L.S. Tomashow & R.J. Cook. 1995. Variation in Sensitivity of Gaeumannomyces graminis to Antibiotics Produced in Fluorescent Pseudomonas spp. and Effect on Biological Control of Take-all of Wheat. Appl. and Environ. Microbiol. 61: 2554─2559.

Michereff, S.J., N.S.S. Silveira, A. Reis & R.L.R. Mariano. 1994. Epiphytic Bacteria Antagonistic to Curvularia Leaf Spot of Yam. Microb. Ecol. 28: 101─1 10.

Schaad, N.W. 1988. Laboratory Guide for Identification of Plant Pathogenic Bacteria. The American Phytopathological Society. St. Paul. 165 p.

Sivamany, E. & S.S. Gnanamanickam. 1988. Biological Control of Fusarium oxysporum f.sp. cubense in Banana by Inoculation with Pseudomonas fluorescens. Plant and Soil 107: 3─9.

Ou, S.H. 1985. Rice Diseases. Mycological Institute, Commonwealth Kew. 382 p.

Vincent, M.N., L.A. Harrison, J.M. Brackin, P.A. Kovacevich, P. Mukerji, D.M. Weller & E.A. Pierson. 1991. Genetic Analysis of the Antifungal Activity of a Soilborne Pseudomomonas aeurofaciens. Appl, and Environ. Microbiol. 57: 2928─2934

Vinther, F. 1985. Laboratory Guide for Seed Bacteriology. Danish Gonvernment Institute for Seed Pathology for Developing Countries. Hellerup. 56 p.

Weller, D.M. 1988. Biological Control of Soilbome Pathogens in the Rhizospere with Bacteria. Ann. Rev. Phytopath. 26: 379─407.

Weller, D.M. & L.S. Thomashow. 1993. Use of Rhizobacteria for Biocontrol. Current Opinion in Biotech. 4: 306─311.



DOI: https://doi.org/10.22146/jpti.10073

Article Metrics

Abstract views : 959 | views : 500

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2001 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics