Eksplorasi, Pengujian, dan Identifikasi Khamir Antagonis terhadap Patogen Antraknos (Colletotrichum lagenarium) pada Semangka

https://doi.org/10.22146/jpti.11964

Kardi Raharjo(1*), Christanti Sumardiyono(2), Nursamsi Pusposendjojo(3), Sismindari Sismindari(4)

(1) PT Prana Ardita Temanggung
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(4) Fakultas Farmasi Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Phyllosphere is one of habitat of yeast and the other microorganism included an antagonistic microorganism. The antagonistic yeasts have been isolated from watermelon phyllosphere on YM Agar medium. There are 30 isolates of yeast have been found. The pure culture of virulent isolate 115 of C. lagenarium used for testing the antagonistic ability of yeast isolates. Two yeast isolates namely K10 and K15 have highest ability to inhibit growth and development of C. lagenarium colony. From microscopic observation, the isolates K10 and K15 caused deflated on C. lagenarium hyphe. The result of identification of K10 is Candida sp. and K15 is Sirobasidium sp.

Keywords


Candida; Sirobasidium; Colletotrichum

Full Text:

PDF


References

Benbow, J.M. & D. Sugar. 1999. Fruit Surface Colonization and Biological Control of Postharvest Diseases of Pear by Preharvest· Yeast Application. Plant Disease 83(6): 839-844.

Cabib, E. 1987. The Synthesis and Degradation of Chitin. In A. Meister ( ed.). Advances in Enzymology. And Related Area of Molecular Biology. John Wiley & Sons. New York, Toronto. 59-101 pp.

Campbell, R. 1989. Biological Control of Microbial Plant Pathogens. Cambridge University Press, Cambridge, New York, Sydney.169-183 pp.

Crawford, D. L. 1999. Mechanism of Biocontrol of Fungal Root Pathogens in The Rhizosphere by Streptomyces lydicus WYEC108. Dissertation Abstracts. University of Idaho. 70 p.

Dickinson, C. H. 1971. Cultural Studies of Leaf Saprophytes. In T. F. Preece and C. H. Dickinson (Eds.). Ecology of Leaf surface Microorganism. Academic Press. London. New York. 129-157 pp.

Elad, Y., D.R. David, T. Levi, A. Kapat, B. Kirshner, E. Guvrin & A. Levfoe.1999. Trichoderma harzianum T 39. Mechanisms of Biocontrol of Foliar Pathogens. In . I. Lyr, P. E. Russell and H. B. Modetn (Eds.). Fungicide and Antifungal Compounds II. 12th International Reinhardsbrunn Symposium May 24th-29th 1999. Friedrichroda, Thuringia, Germany. Intercept, Andover, Rants, SP 10 1 YG, UK. 459-467 pp.

Esquerre-Tugaye, M. T., D. Mazau, J. P. Barthe, C. Lafitte, & A. Touze. 1992. In Colletotrichum. In J. a. Bailey and M. J. Jeger (eds.). Colletrotichum: Biology, Pathology and Control. CAB International. Melksham. 67-87pp.

Jacobsen, B. J. 1997. Role of Plant Pathology in Integrated Pest Management. Annu. Rev. Phytopath. 35: 373-391.

Jeffries.P, & I. Koomen. 1992. Strategies and Prospects for Biological Control of Diseases Caused by Colletotrichum. In J. A. Bailey and M. J. Jeger (Eds.). Colletotrichum. Biology, Pathology And Control. C. A. B. International.Melksham. 337-357 pp.

Johnson, K. B. & J. A.·Dileon. 1999. Effect of Antibiosis on Antagonist Dose Plant Disease Response Relationships for the Biological Control of Crown Gall of Tomato and Cherry. Phytophatology 89(10): 974-980.

Kriger, P. S. 2000. Regulation of Alpha-1,3-glucanase and Other Polysaccharide-degrading Enzymes From Trichoderma harzianum. Dissertation Abstracts. The University of Rochester. 218 p.

Leben, C. 1971. The Bud in Relation to Epiphytic Microflora. In T. F. Preece and C. H. Dickinson (eds.). Ecology of Leaf Surface. Academic Press. New York. 117-127 pp.

Mathivanan, N., V. Kabilan, & K. Murugesan. 2000. Purification, Characterization, and Antifungal Activity of Chitinase from Fusarium chlamydosporum, A Mycoparasite to Groundnut Rust, Puccinia arachidis . Can. J. Microbial. 44(7): 646-651.

Mazzola, M. 1999. Transformation of Soil Microbial Community Structure and Rhizoctonia- Suppressive Potential in Respone to Apple Roots. Phytophatology 89(10): 920-927.

Meyer, S. A., D. G. Ahearn, & D. Yarrow. 1987. Candida Berkhout. In N. J. W. Kregervan Rij (ed.). The Yeast, A Taxonomic Study. Third Revised and Enlarger Edition. Elsevier Science Publishers B. V.Amsterdam. 585-844.

O'Connell, R. J. 1991. Cytochemical Analysis of Infection Structures of Colletotrichum lindemuthianum Using Fluorochrome-labelled Lectins. Physiol. Mo!. Plant Pathol. 39 (5):189-200.

O'Connell, R. J., C. Nash & J. A. Bailey. 1992. Lectin Cytochemistry: A New Approach to Understanding Cell Differentiation, Pathogenesis And Taxonomy. In Colletotrichum. In J. a. Bailey and M. J. Jeger (eds.). Colletrotichum: Biology, Pathology and Control. 67-87 pp.

Ohtakara, A., M. Izume & M. Mitsutorni. 1988. Action Microbial Chitinases on Chitosan with Different Degrees of Deacetylation. Agric. Biol. Chem. 52(12): 3181-3182.

Preece, T. F. ·&.c. H. Dickinson. 1971. Ecology of Leaf Surface Microorganism. Academic Press. London. New York.

Raaijmakers, J.M. R. , R. F. Bonsall, & D. M. Weller. 1999. Relationship Beetween Root Colonization and In Situ Antibiotic Production by Pseudomonas. fluorescens. Phytopathology 89(6): abstract supplement S63.

Sabaratnam, S. 1999. Biological Control of Rhizoctonia Damping-Off of Tomato With a Rhizosphere Actinomycete. Dissertation Abstracts. The University Of Western Ontario, Canada. 209 p.

Selitremiikoff, C. P. 2001. Antifungal Protein. Appl. Environ. Microbial. 67(7):2883-2894pp.

Semangun, H. 1991. Penyakit-penyakit Penting Tanaman Hortikultura Di Indonesia. Gadjah Mada University Press. Yogyakata. 850 p.

Sherf, A. F. & A. A. Macnab. 1986. Vegetable Diseases and Their Control. Second Edition. John Wiley & Sons. New York.

Singh, P. P., Y. C. Shin, G S. Park ,& Y. R. Chung. 1999. Biological Control of Fusarium Wilt of Cucumber by Chitinolytic Bacteria. Phytopathology 89(1 ): 92-99.

Sitterly, W. R. & A. P. Keinath. 1996. Antracnose. In Compendium of Cucurbit Diseases. APS Press. New York.

Suzuki, K., T. Uchiyama, M. Suzuki, N. Nikaidou, M. Regue, & T. Watanabe. 2000. LysR-type Transkripsional Regulator ChiR is Essential for Production of All Chitinase and A Chitin-Binding Protein, CBP 21, in Serratia marcescens 2170. Biosci. Biotechnol. Biochem. 65(2): 338-347.

Tantawi, A. R., A. Harsojo, & H. Serriangun. 1993. Jamur Filoplan Tanaman Karet. Tesis S2 Program Pascasarjana UGM. Yogyakarta.

Tsujibo, H. T. Okamoto, N. Hatano, K. Miyamoto, T. Watanabe, M. Mitsutomi, & Y. lnamori. 2000. Family 19 Chitinase from Streptomyces thermovilaceus OPC-520: Molecular Cloning and Characterization. Biosci. Biotechnol. Biochem. 64(11):2445-2453.

Van derWalt, J.P. & D. Yarrow. 1987. Methods for the Isolation, Maintenance, Classification and Identification of Yeast. In N. J. W. Kreger-van Rij (ed.). The Yeasts. A Taxonomic Study (third revised and enlarged edition). Elsevier Science Publisers B. V. Amsterdam. 45-104pp.

Velazhahan, R., R. Samiyappan, & P. Vidhyasekaran. 2000. Purification of Elicitor Inducible Antifungal Chitinase from Suspension-Cultured Rice Cells. Phytoparasitica 28(2): 1-9.

Wells, H. D. 1986. Trichoderma as A Biocontrol Agent. Dalam K. G. Mukerji and K. L. Garg. CRC Press, Inc, Boca Raton Florida. 71-82 pp.

Xu, T., G. E. Harman, Y. L. Wang, & Y. Shen. 1999. Bioassay of Trichoderma harzianum strains for Control of Rice Sheath Blight. Phytopathology 89(6): abstract supplement S86.

Yedidia, I., N. Benhamou, & I. Chet. 1999. Induction of Defence Respone in Cucumber Plants (Cucumis sativus L.) by The Biocontrol Agent Trichoderma harzianum. Appl. Environ. Microbiol 65(3): I 061-1070.

Yoon, H. G, H. Y. Kim, H. K. Kim, B. S. Hong, D. H. Shin, & H. Y. Cho. 2001. Thermostable Chitosanase From Bacillus sp. Strain CK4: Its Purification, Characterization, and Reaction Patterns. Biosci. Biotechnol. Biochem., 65 (4): 802-809.

Zhang, J. X. ,B. D. Bruton, & C. L. Biles. 1999. Relationship of Cell Wall degrading Enzymes to Virulence of Didymella bryoniae. Phytophatology 89(6): abstract supplement S89.

Zitter, T. A. 1987. Vegetable Crop. An Antracnose of Cucurbits. Cornell Univ. Ithaca, NY. Vegetable MD Online. http://ppathw3. cals. corne/l .edu/extensiases/factsheets/cucurbit antracnose.htm.

Zitter, T. A., D. L. Hopkins, & C. E. Thomas. 1998. Compendium of cucurbit Diseases. APS Press. Minnesota.



DOI: https://doi.org/10.22146/jpti.11964

Article Metrics

Abstract views : 1551 | views : 712

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2006 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics