Interaksi Geminivirus pada Kejadian Inokulasi Ganda

https://doi.org/10.22146/jpti.12105

Sri Hendrastuti Hidayat(1*), Rina Rachmawati(2), Puji Lestari(3), Noor Aidawati(4)

(1) Fakultas Pertanian Institut Pertanian Bogor
(2) Fakultas Pertanian Institut Pertanian Bogor
(3) Fakultas Pertanian Institut Pertanian Bogor
(4) Fakultas Pertanian Institut Pertanian Bogor
(*) Corresponding Author

Abstract


Incidence of geminivirus infection has been caused severe losses on various economically important crops. This losses may cause by single infection or mix infection of several strain of geminiviruses. In this experiment we used two strain of geminiviruses, geminivirus infecting chillipepper from Segunung, West Java and geminivirus infecting tomato from Lembang, West Java (GVToL), to study the effect of geminivirus mix infection on the symptom expression and the capability of each strain to multiply in the host cell. Coefficient of dissimilarity between this two strain of geminiviruses is 7.5. When the two strain of geminiviruses was inoculated together to tomato plants by insect vector; Bemisia tabaci, the symptoms on infected plants is slightly different but less severe than those on plants inoculated with each strain separately. Detection with polymerase chain reaction (PCR) followed by restriction enzyme digest of PCR product revealed that GVToL multiply better in the infected host cell.

Keywords


Bemisia tabaci; geminivirus; polymerase chain reaction

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References

Gilbertson RL, Hidayat SH, Paplomatas EJ, & Rojas MR. 1993. Pseudorecombination between infectious cloned DNA components of tomato mottle and bean dwarf mosaic geminivirus. J. Gen. Virol. 74:23 - 31.

Hidayat SH., Rusli ES, & Aidawati N. 1999. Penggunaan primer universal dalam polymerase chain reaction untuk rnendeteksi virus gemini pada cabai. Di dalam Prosiding Kongres Nasional XV dan Seminar llmiah Perhimpunan Fitopatologi Indonesia. Purwokerto, 16 - 18 September 1999: 355 - 359.

Hill JE, Strandberg JO, Hiebert E, & Lazarowitz SG 1998. Asymetric infectivity of pseudore-combinants of cabbage leaf curl virus and squash leaf curl virus : implications for bipartite geminivirus evolution and movement. Virology 250: 283 - 292.

Mehta P, Wyman JA, Nakhla MK, & Maxwell DP. 1994. Polymerase chain reaction detection of viruliferous Bemisia tabaci (Homoptera : Aleyrodidae) with two tomato-infecting geminivirus. J. Econ. Entomol. 87(5): 1285-1290.

Monci F, Campos SS, Castillo JN, & Moriones E. 2002. A natural recombinant between the geminivirus tomato yellow leaf curl Sardinia virus and tomato yellow leaf virus exhibits a novel pathogenic phenotype and is becoming prevalent in Spanish population. Virology 303: 317-326.

Pico B., Diez MJ,. & Nuez F. 1996. Viral diseases causing the greatest economic losses to the tomato crop. II. The tomato yellow leaf curl virus. A review. Science Horticulture 67: 151-196.

Polston JE, & Anderson PK. 1997. The emergence of whitefly-transmitted geminiviruses in tomato in Western Hemisphere. Plant Dis. 81 (12): 1358-1369.

Rojas MR, Gilbertson RL, Russel DR, & Maxwell DP. 1993. Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminivirus. Plant Dis. 77: 340-347.

Roye ME, Wernecke ME, McLaughin WA, Nakhla MK, & Maxwell DP. 1999. Tomato dwarf leaf curl virus, a new bipartite gerninivirus associated with tomatoes and peppers in Jamaica and mixed infection with tomato yellow leaf curl virus. Plant Pathol 48(3): 370-378.

Sambrook J, Pitsch EF, & Maniatis T. 1989. Molecular cloning : A Laboratory Manual. New York, Cold Spring Harbor Laboratory Press.

Trisusilowati EB., Suseno R, Sosromarsono S, Barizi, Soedarmadi, & Nur MA. 1990. Transmission, serological aspects and morphology of the tobacco krupuk virus. lndon. J. Agric. (2):75-79.

Uzcategui RC, & Lastra R. 1977. Transmission and physical properties of the causal agent of mosaico amarillo del tomate (tomato yellow mosaic). Phytopathology 68: 985-988.

Wyatt SD, & Brown JK. 1996. Detection of subgroup III geminiviruses isolates in leaf extracts by degenerate primer and polymerase chain reaction. Phytopathology 86: I 288-1293.



DOI: https://doi.org/10.22146/jpti.12105

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