Seleksi Resistansi Wereng Batang Padi Coklat terhadap Insektisida Fipronil

Melhanah Melhanah(1*), Witjaksono Witjaksono(2), Y. A. Trisyono(3)

(1) Fakultas Pertanian Universitas Palangka Raya
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author


The rice brown planthoper, Nilaparvata lugens Stal (Homoptera: Delphacidae), is one of  key pests on rice. The use of insecticides unwisely has caused N. lugens to develop resistance. The objective of this research was to determine the potency of N. lugens to develop resistance through laboratory, selections to fipronil, a relatively new insecticide.  A population of N. lugens was collected from rice field in Muntilan, Central Java during the outbreak in 2001. Selections were carried out every generation for four generations against third instars. Selection was started by dipping Cisadane rice seedlings in a solution of fipronil (50 ppm). The concentration was increased every generation, and the concentration used for the last selection (the fourth generation) was 250 ppm. To determine the rate of resistance development, biassays were carried out to estimate LC50 of fipronil against selected and non-selected populations of N. lugens, and their LC50 values were compared to that of the laboratory population. The laboratory population has been in the laboratory for 15 years without any insecticides exposure. The founding population was as susceptible as the laboratory population to fipronil because their LC50 values were similar. Even though the concentration for selections was increased from generation to generation, the survival rate of N. lugens increased steadily. After four generations of selection, the resistance of selected population was 27.3 times than the non-selected population. These findings indicate that N. lugens population has the potency to develop resistance to fipronil in a continuous selection pressure is applied.


Nilaparvata lugens; fipronil; selection resistance

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Atmosudirdjo, 0. 1987. Makalah Resistansi Hama terhadap Insektisida. Makalah disampaikan pada Simposium Nasional Pengelolaan Pestisida Pertanian di Indonesia, 8 - 10 Januari, Yogyakarta.

Chung, T. C., C. N. Sun, & C. Y. Hung. 1982. Resistance of Nilaparvata lugens to Six Insecticides in Taiwan. J. Econ. Entomol. 75: 199 - 200.

Dyck, V. A. & B. Thomas. 1979. The Brown Planthopper Problem, p 3 - 17. In Brown Planthopper: Threat to Rice Production in Asia. International Rice Research Institute, Los Banos Philippines. Finney, DJ. ·1971. Probit Analysis. 3rd ed. Cambridge University Press, London. 333p.

Flexner, J. L., K. M. Theiling, B. A. Croft, & P. H. Westigard. 1989. Fitness and Immigration: Factors Affecting Reversion of Organotin Resistance in The Twospotted spidermite (Acari : Tetranychidae). J. Econ. Entomol. 82: 96- 102.

Hammock, B. D. & D. V. Soderlund. 1986. Chemical Strategies for Resistance Management, p. 111 - 139. In Committee on Strategies for The Management of Pesticide Resistance ( eds.), Pesticide Resistance: Strategics and Tactics for Management. National Academic Press, Washington, D.C.

Lin, Y. H., C. N. Sun, & H. T. Feng. 1979. Resistance of Nilaparvata Lugens to MIPC and MTMC in Taiwan. J. Econ. Entomol. 72: 901 - 903.

Mahrub, E. 2000. Kajian Terjadinya Letusan Populasi Wereng Batang Padi Coklat di Kabupaten Sleman (studi kasus: tingkat pemahaman petani terhadap prinsip dasar PHT). Mediagama II Mei: 26- 32.

Marcon, P. C. R. G., L. J. Young, K. L. Steffey, & B. D. Siegfried. 1999. Baseline Susceptibility of European Corn Borer (Lepidoptera: Crambidae) to Bacillus thuringiensis toxin. J. Econ. Entomol. 92: 279 - 285.

Pathak, M. D. 1977. Insect Pest of Rice, International Rice Research Institute, Los Banos. Philippines. p 16 - 23.

Rifianto, T. 1986. Ketahanan Hama Wereng di Daerah Istimewa Yogyakarta terhadap Beberapa lnsektisida. Skripsi Fakultas Pertanian Universitas Gadjah Mada. Yogyakarta. 57 hal.

Sanches, E. R., Y. Suzuki, K. Miyamoto, & T. Watanabe. 1999. The Potential for Compensation of The Effect of The Brown Planthopper Nilaparvata lugens Stal (Homoptera: Delphacidae) Feeding on Rice. Crop Protec. 18.

Sutrisno. 1987. Resistansi Wereng Coklat Nilaparvata lugens (Stal) terhadap Insektisida di Indonesia. Wereng Coklat Edisi Khusus l: 55 - 68.

Ware, G.P. 2001. An Introduction to Insecticides (3rd ed.). http:// edu/chapters/ware.htm.


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