Pemanfaatan Membran Nitroselulosa untuk Pengiriman Antigen Uji dalam Deteksi TMV dengan DIBA

Susamto Somowiyarjo(1*), Y. B. Sumardiyono(2), Suharno Suharno(3)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author


Simplification of dot immunobinding assay (DIBA) by using TMV-infected samples which were stored and mailed in nitrocellulose membrane (NCM) was described. The antigenicity of TMV in DIBA could be maintained in leaf extract-dotted NCM which had been stored at 29ºC al least for 42 days. The method was developed for sending samples using infected leaf extract-dotted NCM to replace fresh samples. By this method, the antigenicity of the virus could he detected after they have been sent to 18 places which took time from 7 to 26 days. It ts anticipated that the simplicity of DIBA using mailed samples will lead to DIBA's rapid adoption for development of central diagnosis facilities to support the viral diseases management. It may also have wider use in DIBA-based screening and survey programs for plant viruses and could overcome plant quarantine restriction.


TMV; antigenicity; DIBA

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Banttari, E.E. and P.H. Goodwin. 1985. Detection of Potato Virus S, X, and Y by Enzyme-linked Immunosorbent Assay on Nitrocellulose Membrane (Dot-ELISA): Plant Diseases 69: 202-205.

Hartono S., YB. Sumardivono dan S. Somowiyarjo. 1996. Karakterisasi TMV isolat Bawang Putih. BPPS-UGM 9(2B): 167-175.

Hibi, T. and Y. Saito. 1985. A Dot-Immunobinding Assay for the Detection of Tobacco Mosaic Virus in Infected Tissues. J Gen. Virol. 66: 1191-1194.

Lister, R.M .. D. Clement. M. Skaria and J .A. McFatridge. 1985. Stability of ELISA Activity of Barlley Yellow Dwarf Virus in Leaf Samples and Extracts. Plant Disease 69(10): 854-8578.

Matthews, R.E.F. 1970. Plant Virology. Acad. Press., New York. 778 p.

Mori, N., T. Ogawa and N. Matsubara. 1989. Production of Virus-free Garlic (Allium sativum L.) by Tissue Culture. Bull. of Nagasaki Agric. and For. Exp. St. 17: 67-83.

Palfree, R.G.E. and B.E. Elliott. 1982. An Enzyme-linked lmmunosorbent Assay (ELISA) for Detergent Solubilized la Glicoproteins Using Nitrocellulose Membrane Discs. J Immunol. Methods. 52:395-408.

Semangun, H. 1988. Penyakit-penyakit Tanaman Perkebunan di Indonesia. Gadjah Mada Univ. Press. 808p.

Somowiyarjo, S., N. Sako and F. Nonaka. 1989. Dot-immunobinding Assay for Zucchini Yellow Mosaic Virus Using Polyclonal and Monoclonal Antibodies. Ann. Phytopath. Soc. .Japan 55: 56-63.

Somowiyarjo, S., Y.B. Sumardiyono dan P. Widiyati. 1996. Pemendekan Waktu ELISA Dalam Deteksi TMV. J. Perlind. Tan. Ind 2(2): 66-71.

Tsuneyoshi, T. and S. Sumi. 1996. Differentiation among garlic viruses in mixed infections based on RT-PCR procedures and direct tissue blotting immunoassays. Phytopothology 86:253-259.

Van Dijk, P. 1993. Carlavirus Isolates from Cultivated Allium Species Represent Three Viruses. Nth. J Pl. Path. 99: 233-257.

Van Dijk, P. and Sutarya. 1992. Virus survey of garlic, shallot and welsh onion in Java, Indonesia. Internal Communication of the DLO Centre for Plant Breeding and Reproduction Research (CPRO-DLO) Wageningen. Netherlands. 70p.

Walkey, D.G.A. 1990. Virus Diseases. p.191-212. Dalam H.D. Rabinowitch and J.L. Brewster (Ed.) Onions and Allied Crops. Vol. II. CRC Press, INC., Florida.

Walkey, D.G.A. and D.N. Antill. 1989. Agronomic Evaluation of Virus-free and Virus-infected Garlic (Allium sativum L.). J. Horticuitural Science 64: 53-60.

Yamashita, K. and K. Oikawa. 1992. Detection of Garlic Mosaic Virus by DIBA and Indirect ELISA. Ann Rept. Plant Prot. North Japan 43:66-68.


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