* Corresponding Author
Infeksi Ganda Begomovirus dan Crinivirus pada Tanaman Tomat di Kabupaten Magelang, Jawa Tengah
https://doi.org/10.22146/jpti.17542 Fitri Kusumaningrum(1*), Sedyo Hartono(2), Sri Sulandari(3), Susamto Somowiyarjo(4)
(1) Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada, Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(2) Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada, Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(3) Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada, Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(4) Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada, Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author
Abstract
Since 2006, a yellowing disease has been observed in tomato (Lycopersicon esculentum) fields in Central Java, Indonesia. Epidemics of the diseases were mainly associated with populations of the whitefly Trialeurodes vaporariorum and Bemisia tabaci, the major whitefly pests in vegetable crops. The main symptoms were severe yellowing on lower leaves, and curling on upper leaves of plant. Total DNA was extracted from tomato leaves using CTAB methods, while total RNA was extracted using NucleoSpin RNA Plant extraction kit (Macherey-Nagel). Because of occurring mixed symptom on an individual plant, hereby it is important to detect the causal agent to manage of the disease. Samples from symptomatic plants were analyzed by polymerase chain reaction (PCR) and shown to be infected with Tomato infectious chlorosis virus (TICV) (family Closteroviridae, genus Crinivirus) and a virus species belongs to the genus Begomovirus, family Geminiviridae. The research result is the first report of Crinivirus and Begomovirus double infection in single tomato fields in Indonesia.
INTISARI
Penyakit kuning pada tanaman tomat (Lycopersicon esculentum) telah ditemukan sejak tahun 2006 di Jawa Tengah, Indonesia. Epidemi penyakit tersebut terutama berkaitan dengan keberadaan dua spesies whitefly (Trialeurodes vaporariorum dan Bemisia tabaci). Gejala utama yang ditemukan adalah daun-daun pada tanaman bagian bawah berwarna kuning sedangkan daun daun pada tanaman bagian atas menunjukkan gejala keriting. Adanya gejala campuran pada satu individu tanaman maka perlu dideteksi penyebabnya untuk pengelolaannya. DNA total diekstraksi dari daun tomat yang terinfeksi menggunakan metode CTAB, sedangkan total RNA diekstraksi dengan menggunaan NucleoSpin RNA Plant extraction kit (Macherey-Nagel). Analisis sampel tanaman sakit menggunakan teknik poymerase chain reaction (PCR) menunjukkan adanya infeksi Tomato infectious chlorosis virus (TICV) (famili Closteroviridae, genus Crinivirus) dan satu spesies virus anggota genus Begomovirus, famili Geminiviridae. Hasil penelitian ini merupakan laporan pertama kali adanya infeksi ganda kelompok Crinivirus dan Begomovirus pada satu individu tanaman tomat di Indonesia.
Keywords
Full Text:
PDFReferences
Amir. 2009. Kajian Penularan dan Respon Ketahanan Beberapa Varietas Tomat terhadap Tomato infectious chlorosis virus (TICV). Tesis. Program Pascasarjana, Fakultas Pertanian Universitas Gadjah Mada, Yogyakarta. 42 p. (Tidak dipublikasikan).
Anonim. 2003. Distribution of Bemisia tabaci in Latin America. Presented in May at the VIIIth International Plant Virus Epidemiology Symposium in Ascherleben, Germany.http://gisweb.ciat.cgiar. org/SIG/download/AR2003/annual_report_3_5.pdf. 5 p, diakses 14/8/10.
Hartono, S., T. Natsuaki, S. Haruki, H. Atharahi, & S. Okuda. 2003. Yellowing Disease of Tomatoes Caused by Tomato Infectious Chlorosis Virus Newly Recognized in Japan. Journal of General Plant Pathology 69: 61–64.
Hartono, S. 2008. Molecular Identification of Begomovirus Causing Yellow Leaf Curl Disease on Tomato Plants in Central Java. Journal of Acta Agrosia 11: 69−74.
Hartono, S. & A. Wijonarko. 2007. Molecular Characterization of Tomato Infectious Chlorosis Virus Caused Yellowing Disease on Tomato Plants in Indonesia. Journal of Acta Agrosia 9: 139−146.
Hidayat, S.H. 2003a. Rangkuman Hasil Penelitian Geminivirus di Indonesia: Sebagai Bahan Diskusi untuk Menghadapi Peningkatan Infeksi Geminivirus pada Cabai. Disampaikan pada Seminar Sehari Penyakit yang Disebabkan oleh Virus pada Cabai. Direktorat Jendral Perlindungan Tanaman Hortikultura, Jakarta 20 Februari 2003.
Hidayat, S.H. 2003b. Pengenalan dan Pengendalian Penyakit Virus Kuning pada Cabai. Disampaikan dalam Pertemuan Penanggulangan Penyakit Virus Cabai, Direktorat Perlindungan Hortikultura. Dirjen Bina Produksi Hortikultura. Departemen Pertanian di Bandar Lampung, 25 September 2003.
Li, R., S. Salih, & S. Hurtt. 2004. Detection of Gemini viruses in Sweetpotato by Polymerase Chain Reaction. Plant Disease 88: 1347−1351.
Liu, H., G.C. Wisther, & J.E. Duffus. 1998. Comparison of Diagnostic Techniques for Detecting Tomato Infectious Chlorosis Virus. Plant Disease 82: 84−88.
Liu, H., G.C. Wisther, & J.E. Duffus. 2000. Particle Lenght of Whitefly Transmitted Crinivirus. Plant Disease 84: 803−805. Nakhla, M.K. & D.P. Maxwell. 1998. Epidemiology and Management of Tomato yellow leaf curl Disease, p. 565−583. In A. Hadidi, R.K. Khetarpal, H. Koganezawa (eds.), Plant Virus Disease Control. APS Press, St Paul, Minnesota, USA.
Ohnishi, J., T. Kitamura, F. Terami, & K. Honda. 2009. A Selective Barier in the Midgut Epithelial Cell Membrane of the Nonvector Whitefly Trialeurodes vaporariorum to Tomato yellow leaf curl virus Uptake. Plant Disease 75: 131−139.
Polston, J.E. & P.K. Anderson. 1997. The Emergence of Whitefly - Transmitted Geminiviruses in Tomato in Western Hemisphere. Plant Disease 81: 1358−1369.
Purwati, E. & Khairunisa. 2007. Budidaya Tomat Dataran Rendah. Penebar Swadaya. Depok. 67 p.
Revill, P.A., C.V. Ha, S.C. Porchun, M.T. Vu, & J.L. Dale. 2003. The Complete Nucleotide Sequence of Two Distinct Geminiviruses Infecting Cucurbite in Vietnam. Archives of Virology 148: 1523-1541.
Sudiono, S.H. Hidayat, R. Suseno & S. Sosromarsono. 2001. Deteksi Molekuler dan Uji Kisaran Inang Virus Gemini Asal Tanaman Tomat. Prosiding Kongres dan Seminar Nasional Perhimpunan Fitopatologi Indonesia XVI. Bogor, Jawa Barat.
Sulandari, S. 2006. Penyakit Daun Keriting Kuning Cabai di Indonesia. Jurnal Perlindungan Tanaman Indonesia. 12: 1−12.
Tian, T., V.A. Klassen, J.Seong, G. Wisler, J.E. Duffus, & B.W. Falk. 1996. Generation of cDNAs Specific to Lettuce Infectious Yellows Closterovirus and Other Whitefly-transmitted Viruses by RT-PCR and Degenerate Oligonucleotide Primers Corresponding to Closterovirus Gene Encoding the Heat Shock Protein 70 Homolog. Phytopathology 86: 1167− 1173.
Wisler, G.C., H.Y. Liu, V.A. Klaassen, J.E. Duffus, & B.W. Falk. 1998. Tomato Infectious Chlorosis Virus Has a Bipartite Genome and Induces Phloem –Limited Inclusions Characteristic of the Clostero-viruses. The American Phytopathologycal Society 86: 622−626.
DOI: https://doi.org/10.22146/jpti.17542
Article Metrics
Abstract views : 5158
| 
views : 3578
Refbacks
- There are currently no refbacks.
Copyright (c) 2017 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics