Morphological and Molecular Observation to Confirm the Taxonomic of Coptocercus biguttatus (Coleoptera: Cerambycidae) on Cloves in Ambon and Part of Ceram Island

https://doi.org/10.22146/jpti.25748

Mohamad Pamuji Setyolaksono(1*), Suputa Suputa(2), Nugroho Susetya Putra(3)

(1) Regional Estate Crop Office on Seed Management and Plant Protection, Ambon Jln. Pertanian-Passo, Ambon 97232
(2) Department of Crop Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(3) Department of Crop Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author

Abstract


This research was conducted to confirm the species of longhorn beetle (Coptocercus biguttatus) drilling clove stems in Ambon and part of Ceram Island, Moluccas, which has been noted as an important pest. Aim of this investigation was to characterize the species morphologically, and more detailed with molecular technique via mtCO1 gene analysis. The longhorn beetle was taken in Ambon and part of Ceram Island, Moluccas and then was etablished in laboratory with host rearing method on pieces of clove stem. The results showed that C. biguttatus attacking clove stems in Ambon and part of Ceram Island, Maluku was closely related and grouped into same cluster with C. rubripes and P. semipunctata in of New Zealand with 85% homology value. C. biguttatus distributed evenly in all clove planting areas in Ambon and part of Ceram Island.

 

Intisari

Penelitian ini dilakukan untuk mengonfirmasi spesies kumbang sungut panjang (Coptocercus biguttatus) yang menggerek batang tanaman cengkih di Pulau Ambon dan sebagian dari Pulau Seram, Maluku menggunakan karakter morfologi dan molekuler berbasis gen mtCO1 untuk sidik kekerabatan. Pengambilan sampel kumbang sungut panjang dilakukan di Pulau Ambon dan sebagian dari Pulau Seram, Maluku dengan cara host rearing pada potongan batang cengkih. Hasil penelitian menunjukkan bahwa C. biguttatus di Pulau Ambon dan sebagian dari Pulau Seram, Maluku berkerabat dekat dan berada dalam satu kelompok dengan C. rubripes dan P. semipunctata di New Zealand dengan nilai homologi 85%. Penyebaran C. biguttatus di Pulau Ambon dan sebagian dari Pulau Seram merata di seluruh area pertanaman cengkih.


Keywords


Cerambycidae; clove stem borer; Coptocercus biguttatus; Maluku; Mollucas; penggerek batang cengkih

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References

Amirullah, C. Ariani, & Suriana. 2014. Keaneka-ragaman Kumbang Cerambycidae (Coleoptera) di Kawasan Gunung Mekongga Desa Tinukari Kecamatan Wawo Kabupaten Kolaka Utara Provinsi Sulawesi Tenggara [Diversity Cerambycidae Beetles (Coleoptera) in Mekongga Mountain Area Tinukari Village Wawo North Kolaka District of Southeast Sulawesi Province]. Biowallacea 1: 16−24.

Boa, E. 1990. Clove Disease Project: Summary of Research 1975−1990. Publications by the Clove Diseases Project (ATA-71) and Associated Scientists. Balittro, Bogor. 36 p.

Brockerhoff, G.E. & J. Bain. 2000. Biosecurity Implications of Exotic Beetles Attacking Trees and Shrubs In New Zealand. New Zealand Plant Protection 53: 321−327.

Dhahri S., F. Lieutier, F.C. Cheikhrouha, & M.L. Ben Jamaa. 2016. Distribution, Preference and Performance of Phoracantha recurva and Phoracantha semipunctata (Coleoptera Cerambycidae) on Various Eucalyptus Species in Tunisia. REDIA 99: 83−95.

Dharmayanti, N.L.P.I. 2011. Filogenetika Molekuler: Metode Taksonomi Organisme Berdasarkan Sejarah Evolusi [Molecular Phylogenetic: Organism Taxonomy Method Based on Evolutionary History]. Wartazoa 21: 1−10.

Fahri & M. Sataral. 2015. Longhorn Beetle (Coleoptera: Cerambycidae) in Enclave Area, Lore Lindu National Park, Central Sulawesi. Online Journal of Natural Science 4: 149−157.

Folmer, O., M. Black, W. Hoeh, R. Lutz, & R. Vrijenhoek. 1994. DNA Primers for Amplification of Mitochondrial Cytochrome C Oxidase Subunit I from Diverse Metazoan Invertebrates. Molecular Marine Biology and Biotechnology 3: 294−299.

Goodwin, P.H., B.G. Xue, C.R. Kuske, & M.K. Sears. 1994. Amplification of Plasmid DNA to Detect Plant Pathogenic Mycoplasmalike Organisms. Annals of Applied Biology 124: 27−36.

Gressitt, J.L. 1951. Longicorn Beetles from New Guinea and the South Pacific (Coleoptera: Cerambycidae). Annals of the Entomological Society of America 44: 1−30.

Hanks, L.M., T.D. Paine, & J.G. Millar. 1991. Mechanisms of Resistance in Eucalyptus against Larvae of the Eucalyptus Longhorned Borer (Coleoptera: Cerambycidae). Entomological Society of America 20: 1583−1588.

Hanks, L.M., T.D. Paine, & J.G. Millar. 1993. Host Species Preference and Larval Performance in the Wood-Boring Beetle Phoracantha semipunctata F. Oecologia 95: 22−29.

Hanks, L.M., T.D. Paine, J.G. Millar, C.D. Campbell, & U.K. Schuch. 1999. Water Relations of Host Trees and Resistance to the Phloem-Boring Beetle Phoracantha semipunctata F. (Coleoptera: Cerambycidae). Oecologia 119: 400−407.

Hiroshi, M., & W.A. Noerdjito. 2004. Longicorn Beetles of Museum Zoologicum Bogoriense, Identified by Dr. E.F. Gilmour, 1963 (Coleoptera: Disteniidae and Cerambycidae). Bulletin of FFPRI 3: 49-98.

Kalshoven L.G.E. 1981. Pest of Crops in Indonesia. (Revised and Translated by PA Van Der Laan). PT. Ichtiar Baru, Jakarta. 701 p.

Kumar, S., G. Stecher, & K. Tamura. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0. Journal Molecular Biology and Evolution 33: 1870−1874.

Makihara H., Sugiharto, & W.A. Noerdjito. 2011. Longicorn Beetles from Mts. Bromo in East Java, Indonesia, with Descriptions of a New Species and a New Subspecies (Coleoptera; Cerambycidae). Bulletin of FFPRI 10: 251−261.

Makihara H., W.A. Noerdjito, & Sugiharto. 2002. Longicorn Beetles from Gunung Halimun National Park, West Java, Indonesia from 1997−2002 (Coleoptera; Disteniidae and Cerambycidae). Bulletin of FFPRI 1: 189−223.

Miroshnikov, A.I. 2016. New Data on the Taxonomy and Geographic Distribution of Oriental Species of the Genus Coptocercus Hope, 1841 (Coleoptera: Cerambycidae: Phoracanthini). Russian Entomological Journal 25: 387−392.

Morillo, F., P. Sanchez, C. Giron, A. Valera, W. Munoz, & J. Guerra. 2008. Behavior of Cacao Hybrids (Theobroma cacao) to Attack by Steirastoma breve (Coleoptera: Cerambycidae). Revista Colombiana de Entomologia 34: 151−155.

Noerdjito, W.A. 2010. Arti Kebun Raya Bogor bagi Kehidupan Kumbang Sungut Panjang (Coleoptera, Cerambycidae) [Meaning of the Bogor Botanical Garden for Life Longhorn Beetles (Coleoptera, Cerambycidae)]. Jurnal Biologi Indonesia 6: 289−292.

Noerdjito, W.A. 2011. Evaluasi Kondisi Hutan Berdasarkan Keragaman Kumbang Sungut Panjang (Coleoptera; Cerambycidae) di Kawasan Gunung Slamet [Evaluation of Forest Condition Based on Diversity of Longhorn Beetle (Coleoptera; Cerambycidae) in Slamet Mountain Area]. Berita Biologi 10: 521−531.

Pool, P.A., S.J. Eden-Green, & M.T. Muhammad. 1986. Variation in Clove (Syzygium aromaticum) Germplasm in the Moluccan Islands. Euphytica 35: 149−159.

Putra, I.G.A.P., N.L. Watiniasih, & N.M. Suartini. 2011. Inventarisasi Serangga pada Perkebunan Kakao (Theobroma cacao) Laboratorium Unit Perlindungan Tanaman Desa Bedulu, Kecamatan Blahbatuh, Kabupaten Gianyar, Bali [Inventory of Insects on Cocoa Plantation (Theobroma cacao) Laboratory of Plant Protection Unit, Bedulu Village, Blahbatuh District, Gianyar Regency, Bali]. Jurnal Biologi 14: 19−24.

Rhainds, M., L. Chinchiew, Z. Moli, & G. Gries. 2002. Incidence, Symptoms, and Intensity of Damage by Three Coffee Stemborers (Coleoptera: Cerambycidae) in South Yunan, China. Journal of Economic Entomology 95: 106−112.

Rusterholz, H.P., S. Ursenbacher, A. Coray, U. Weibel, & B. Baur. 2015. DNA Quantity and Quality in Remnants of Traffic-Killed Specimens of an Endangered Longhorn Beetle: A Comparison of Different Methods. Journal of Insect Science 15: 120−125.

Sataral, M., T. Atmowidi, & W.A. Noerdjito. 2015. Diversity and Abundance of Longhorn Beetles (Coleoptera: Cerambycidae) in Gunung Walat Educational Forest, West Java, Indonesia. Journal of Insect Biodiversity 3: 1−12.

Sharma, P., & T. Kobayashi. 2014. Are “Universal” DNA Primers Really Universal?. Journal of Applied Genetics-Springer 55: 485−497.

Sopow, S., B. Gresham, & J. Bain. 2015. Exotic Longhorn Beetles (Coleoptera: Cerambycidae) Established in New Zealand. New Zealand Entomologist 38: 107−125.

Wang, Q. 1998. The Phoracanthine Beetles (Coleoptera: Cerambycidae: Cerambycinae): Taxonomic Overview and Generic Relationships. Invertebrate Taxonomy 12: 667−684.

Wang, Q., I.W.B. Spineton, & T.R. New. 1996. Biogeography of the Phoracanthine Beetles (Coleoptera: Cerambycidae). Journal of Biogeography 23: 75−94.



DOI: https://doi.org/10.22146/jpti.25748

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