* Corresponding Author
Selection and Inheritance of Tomato Resistance against Ralstonia solanacearum
https://doi.org/10.22146/jpti.35464 Isna Maulida(1), Rudi Hari Murti(2*), Triwidodo Arwiyanto(3)
(1) Department of Agronomy, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(2) Department of Agronomy, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author
Abstract
Ralstonia solanacearum is a plant pathogen causes wilting which is a major obstacle in the cultivation of tomato plants. In plant breeding, knowledge of the source of resistance genes and inheritance patterns is important in the development of bacterial wilt resistant varieties. This study aimed to obtain bacterial wilt resistant lines and to find out the inheritance pattern of tomato resistance to bacterial wilt. Selection of resistant plant involved the selected breeding lines from irradiation and crossing collections of the Genetic Laboratory, Faculty of Agriculture, Universitas Gadjah Mada. Introduced lines of H-7996 and F1 Permata and Timoti were used as a control. H-7996 as resistant parents and GM2 as susceptible parents, and their offspring include F1 GM2 x H-7996, F1 reciprocal, F2, Back Cross 1 (F1 x GM2), and Back Cross 2 (F1 x H-7996) used in testing inheritance patterns. Inoculation was carried out 1 week after planting by pouring 100 ml of water suspension of R. solanacarum (108 cfu/ml) on the roots. Completely Randomized Design (CRD) was used in this experiment. The scoring observation was carried out every week for one month. This study showed that Permata as a control was the most resistant, while Timoti and H-7996 were medium resistant. The CLN, G6, G8, and G7 lines were susceptible medium, yet only G8 and G7 with the smallest percentage of disease intensity and not significantly different than Timoti. The resistance gene to bacterial wilt on H-7996 was controlled by genes in the cell nucleus with additive-dominant gene action. Resistance to bacteria has a moderate level of heritability.
Keywords
Full Text:
PDFReferences
Acquaah, G. 2007. Principles of Plant Genetics and Breeding. Blackwell Publishing. USA. 569 p.
Arwiyanto, T., M. Goto, & S. Tsuyumu, S. 1994. Biological Control of Bacterial Wilt of Tomato by an Avirulent Strain of Pseudomonas solanacearum. Annals of Phytopathological Society of Japan 60: 421−430.
Arwiyanto, T. 2014. Ralstonia solanacearum. Biologi, Penyakit yang Ditimbulkan, dan Pengelolaannya. Gadjah Mada University Press, Yogyakarta. 99 p.
Arwiyanto, T., S.D. Nurcahyanti, D. Indradewa, & J. Widada. 2015. Grafting of Local Commercial Tomato Varieties with H-7996 and Eg-203 to Suppress Bacterial Wilt (Ralstonia solanacearum) in Indonesia. Acta Horticultura 1069: 173−178.
Champoiseau, P.G. & T.M. Momol. 2009. Bacterial Wilt of Tomato. https://www.researchgate.net/publication/267829100_Bacterial_Wilt_of_Tomato, modified 4/5/17.
Grimault, V., P. Prior, & G. Anais. 1995. A Monogenic Dominant Resistance of Tomato to Bacterial Wilt in Hawaii 7996 is Associated with Plant Colonization by Pseudomonas solanacearum. Journal of Pytopathology 143: 349−352.
Hai, T.T.H., E. Esch, & J. F. Wang. 2008. Resistance to Taiwanese Race 1 Strain of Ralstonia solanacearum in Wild Tomato Germplasm. European Journal of Plant Pathology 122: 471−479.
Harjadi, S.S. & H. Halim. 1980. Pengujian Heterosis Tanaman Tomat Silangan Resiprokal Av-Gondol dan Av-Apel Belgi untuk Sifat Hortikultura dan Resistensinya Terhadap Penyakit Layu Bakteri. Buletin Agronomi 8: 21−40.
Hayward, A.C. 1991. Biology and Epidemiology of Bacterial Wilt Caused by Pseudomonas solanacearum. Annual Review of Phytopathology 29: 65−87.
Janaki, V. & T. B. Putturaju. 2012. Studies on the Percent Incidence and Reaction of Tomato Cultivar on Bacterial Wilt. International Journal of Plant Protection 5: 175−176.
Kim, S.G., O.S. Hur, N.Y. Ro, H.C. Ko, J.H. Rhee, J.S. Sung, K.Y. Ryu, S.Y. Lee, & H.J. Baek. 2016. Evaluation of Resistance to Ralstonia solanacearum in Tomato Genetic Resources at Seedling Stage. Plant Pathology Journal 32: 58−64.
Laeshita, P. & T. Arwiyanto. 2017. Resistance Test of Several Tomato Varieties to Bacterial Wilt Diseases Caused by Ralstonia solanacearum. Jurnal Perlindungan Tanaman Indonesia 21:51−53.
Lebeau, A., M.C. Daunay, A. Palloix, J.F. Wang, J. Dintinger, F. Chiroleu, E. Wicker, & P. Prior. 2011. Bacterial Wilt Resistance in Tomato, Pepper, and Eggplant: Genetic Resources Respond to Diverse Strain in The Ralstonia solanacearum Species Complex. Phytopathology 101: 154−165.
Lowe-Power, T.M., C.G. Hendrich, E.V. Roepenack-Lahaye, B. L.D. Wu, R. Mitra, B. L. Dalsing, P. Ricca, J. Naidoo, D. Cook, A. Jancewicz, P. Masson, B. Thomma, T. Lahaye, A.J. Michael, & C. Allen. 2017. Metabolomics of Tomato Xylem Sap during Bacterial Wilt Reveals Ralstonia solanacearum Produces Abundant Putrescine, A Metabolite that Accelerates Wilt Disease. Environmental Microbiology 20: 1−49.
Mather, S.K. & J.L. Jinks. 1982. Biometrical Genetics The Study of Continous Variation. 3th Ed. Springer, US. 396 p.
Nawangsih, A.A. 2005. Biological Control of Tomato Bacterial Wilt, Ralstonia solanacearum, by Bacillus. Journal ISSAAS 11: 91−102.
Opena, R.T., G.L. Hartman, & J.T. Chen. 1994. Breeding for Bacterial Wilt Resistance in Tomato: Number of Backcrosses to Recover Adequate Resistance, p. 71−76. In AVRDC (ed.), Plant Breeder and Director of Crop Improvement Programme. Asian Vegetable Research and Development Center, Taiwan.
Rao, M. V.B., H.S. Sohi, & S.K. Tikoo. 1975. Reaction of Wild Resistant Tomato Varieties and Lines to Pseudomonas solanacearum in India. Plant Disease Reporter 59: 734−736.
Robert, D.P., T.P. Denny, & M.A. Schell. 1988. Cloning of the egl Gene of Pseudomonas solanacearum and Analysis of its Role in Phytopathogenicity. Journal of Bacteriology 170:1445−1451.
Seah, S., A.C. Telleen, & V. M. Williamson. 2007. Introgressed and Endogenous Mi-1 Gene Clusters in Tomato Differ by Complex Rearrangements in Flanking Sequences and Show Sequence Exchange and Diversifying Selection among Homologues. Theoretical and Applied Genetics 7: 1289−1302.
Sikirou, R., F. Beed, V. Ezin, J. Hoteigni, & S.A. Miller. 2017. Distribution, Pathological and Biochemical Characterization of Ralstonia solanacearum in Benin Rachidatou. Annals of Agricultural Science 62: 83–88.
Singh, R.K. & B.D. Chaudhary. 1977. Biometrical Methods in Quantitative Genetic Analysis (Revised Edition). Karyani Publisher, New Delhi. 288 p.
Soesanto, L., E. Mugiastuti, & R.F. Rahayuniati. 2011. Inventarisasi dan Identifikasi Patogen Tular-tanah pada Pertanaman Kentang di Kabupaten Purbalingga. Jurnal Hortikultura 21: 254−264.
Stansfield, W.D. 1991. Genetics (Genetika, translated by M. Apandi and L.T. Hardi). 2nd ed. Erlangga, Jakarta. 417 p.
Thakur, A.K., U.K. Kohli, & M. Kumar. 2004. Inheritance of Resistance to Bacterial Wilt in Tomato. Indian Journal Genetics and Plant Breeding 64: 79−80.
Wang, J.F., J. Oliver, P. Thoquet, B. Mangin, L. Sauviac, & M.H. Grimsley. 2000. Resistance of Tomato Line Hawaii 7996 to Ralstonia solanacearum Pss4 in Taiwan is Controlled Mainly by a Major Strain-Specific Locus. Molecular Plant-Microbe Interactions 13: 6−13.
Wang, J.F., F.I. Ho, H. T.H. Truong, S.M. Huang, C.H. Baletero, V. Dittapongpitch, & N. Hidayati. 2013. Identification of Major QTLs Associated with Stable Resistance of Tomato Cultivar ‘Hawaii 7996’ to Ralstonia solanacearum. Euphytica 190: 241–252.
Warner, J. N. 1952. A Method for Estimating Heritability. Agronomy Journal 44: 427−430.
Yuliar, Y. A. Nion, & K. Toyota. 2015. Recent Trends in Control Methods for Bacterial Wilt Diseases Caused by Ralstonia solanacearum. Microbes Environment 30: 1−11.
DOI: https://doi.org/10.22146/jpti.35464
Article Metrics
Abstract views : 2867
| 
views : 2307
Refbacks
- There are currently no refbacks.
Copyright (c) 2019 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics