Molecular Detections and Resistance Response of Six Rice Varieties to Tungroviruses from South Sulawesi

https://doi.org/10.22146/jpti.47355

Saipul Abbas(1*), Sri Sulandari(2), Sedyo Hartono(3), Y. Andi Trisyono(4)

(1) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1 Bulaksumur, Sleman, Yogyakarta 55281
(2) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1 Bulaksumur, Sleman, Yogyakarta 55281
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1 Bulaksumur, Sleman, Yogyakarta 55281
(4) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1 Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author

Abstract


The suspected rice virus is found in the field, namely the tungrovirus which is transmitted by green leafhoppers (Nephotettix virescens). The study aimed to detect the tungrovirus molecularly and examine the resistance response of six rice varieties from the transmission of tungrovirus samples from South Sulawesi on a greenhouse scale. Based on the results of molecular detection with RTSV PCR of the double infected sample with DNA bands 1115 bp and RTBV of around 430 bp, Sidrap, and Maros samples were infected by 430 bp size RTBV, while Wajo sample was not detected by both viruses. The results of RTBV sequence analysis showed that the grouping of Sidrap was still one group with Maros and Pinrang samples and different from the group of samples from Malaysia, Thailand, and Philippines. While the grouping of RTSV shows that Pinrang samples are still one group with samples from Bali, Subang, and different from those of the Philippines, India, and Malaysia. The results of transmission in the greenhouse on six rice varieties (TN1, Ciherang, Mekongga, Tukad Unda, Inpari 36, Inpari 37) showed different plant resistance responses such as susceptible, moderately resistant, and resistant reactions based on the amount of disease intensity caused. Varieties that are classified as susceptible are TN1 and Ciherang varieties, moderately resistant, namely Mekongga and Tukad Unda varieties, and resistant varieties namely Inpari 36 and Inpari 37 varieties.


Keywords


resistance responses; South Sulawesi; tungrovirus detections; varieties

Full Text:

PDF


References

Azzam, O. & T.C.B Chancellor. 2002. The Biology, Epidemiology, and Management of Rice Tungro Disease in Asia. Plant Disease 86: 88-100.

[BPS] Badan Pusat Statistik. 2018. Luas Panen dan Produksi Padi di Indonesia. No. 83/10/Th. XXI, 24 Oktober 2018. Badan Pusat Statistik, Jakarta. https://www.bps.go.id/pressrelease.html, modified 10/10/2019.

Cabauatan, P.Q., U. Melcher, K. Ishikawa, T. Omura, H. Hibino, H. Koganezawa, & O. Azzam. 1999. Sequence Changes in Six Variants of Rice tungro bacilliform virus and their Phylogenetic Relationships. Journal of General Virology 80: 2229–2237.

Choi, I.R., P.Q. Cabauatan, & R.C. Cabunagan. 2009. Rice Tungro Disease. Rice Fact Sheet, IRRI, Sep. 2009: 1–4.

Hasanuddin, A. 2002. Pengendalian Penyakit Tungro Terpadu: Strategi dan Implementasi. Orasi Pengukuhan Ahli Peneliti Utama. Badan Litbang Pertanian, Jakarta.

Hibino, H., M. Roechan, & S. Sudarisman. 1978. Association of Two Types of Virus Particles with Penyakit Habang (Tungro Disease) of Rice in Indonesia. Phytopathology 68: 1412-1416.

Hibino, H., & R.C. Cabunagan. 1986. Rice Tungro Associated Viruses and their Relation to Host Plants and Vector Leafhopper. Tropical Agriculture Research19: 173−182.

[IRRI] International Rice Research Institute. 1996. Standard Evaluation System for Rice. 4th Edition July 1996 INGER Genetic Resources Center, Manila, The Philippines. 52 p.

King, A.M.Q., M.J. Adam, E.B. Carstens, & E.J. Lefkowitz. 2012. Virus Taxonomy Classification and Nomenclature of Viruses: Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, Birmingham (US).1327 p.

Mukhopadhyay, A.N. 1995. Rice Tungro. p. 429--444. In U.S. Sing, A.N. Mukhopadhyay, J. Kumar, H.S.Chaube (eds.), Plant Disease of International Importance. Vol. 1. Disease of Cereals and Pulse. Prentice May, New Jersey.

Omura,  T.,  Y. Saito,  T.Usugi,  &  H.  Hibino.  1983. Purification and  Serology of  Rice tungro spherical and Rice tungro bacilliform viruses. The Phytopathological Society of Japan  49: 73--76.

Pakki, S. 2011. Variabilitas Penyakit Tungro pada Beberapa Varietas Unggul Padi Inbrida di Wilayah Endemis. p. 1--8. Seminar dan Pertemuan Tahunan XXI PEI, PFI Komda Sulawesi Selatan dan Dinas Perkebunan Pemerintah Provinsi Sulawesi Selatan. Makassar, 7 Juni 2011.

Praptana, R.H. & A. Muliadi. 2005. Ketahanan Sepuluh Varietas Padi Lokal Nusa Tenggara Barat (NTB) terhadap Tungro. p. 85--88. In Prosiding Seminar Ilmiah dan PertemuanTahunan PBJ dan PFJ XVJ Komda Sulawesi Selatan. Loka Penelitian Penyakit Tungro. Sulawesi Selatan, 15-20 Mei 2005.

Praptana, R.H., A. Bastian, & M. Yasin. 2005. Penyakit Tungro dan Pengendaliannya. p.53--56. In ProsidingSeminar Ilmiah dan Pertemuan Tahunan PBJ dan PFJ XVJ Komda Sul-Sel. 15-20 Mei 2005.

Raga, I.N., W. Murdita, M.P.L. Tri, S.W. Edi, & Oman. 2004. Sistem Surveillance Antisipasi Ledakan Penyakit Tungro di Indonesia, p. 49--59. In A. Hasanuddin, I.N. Widiarta & Sunihardi (eds.), Strategi Pengendalian Penyakit Tungro: Status dan Program, Prosiding Seminar Nasional Status Program Penelitian Tungro Mendukung Keberlanjutan Produksi Padi Nasional. Makassar, 7-8 September 2004.

Shen, P., M. Kaniewska, C. Smith, & R.N. Beachy. 1993. Nucleotide Sequence and Genomic Organisation of Rice tungro spherical virus. Virology 193: 621-630.

Takahashi, Y., E.R. Tiongco, P.Q. Cabauatan, H. Koganezawa, H. Hibino, & T. Omura. 1993. Detection of Rice tungro bacilliform virus by Polymerase Chain Reaction for Assessing Mild Infection of Plants and Viruliferous Vector Leafhoppers. Phytopathology 83: 655-655.

Widiarta, I.N. 2005. Wereng Hijau (Nephotettix virescens Distant.): Dinamika Populasi dan Strategi Pengendaliannya sebagai Vektor Penyakit Tungro. Jurnal Litbang Pertanian 24: 85-92.

Zadoks, C.J. & R.D. Schein. 1979. Epidemiology and Plant Disease Management. Oxford University Press. New York. 427 p.



DOI: https://doi.org/10.22146/jpti.47355

Article Metrics

Abstract views : 794 | views : 435

Refbacks





Copyright (c) 2020 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 height=auto

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print)ISSN 2548-4788 (online) is published by Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics