Phytonematodes Community and Polyphasic Character of Aphelenchoides varicaudatus on Garlic Plants in Tegal Regency, Central Java

Mokhammad Danang Kusuma(1), Supramana Supramana(2*), Giyanto Giyanto(3)

(1) Department of Plant Protection, Faculty of Agriculture, IPB University IPB Dramaga, Bogor, West Java, 16680
(2) Department of Plant Protection, Faculty of Agriculture, IPB University IPB Dramaga, Bogor, West Java, 16680
(3) Department of Plant Protection, Faculty of Agriculture, IPB University IPB Dramaga, Bogor, West Java, 16680
(*) Corresponding Author


The interception of Ditylenchus dipsaci, D. destructor, and Aphelenchoides fragariae, which are classified as quarantine pests, has been reported to occur on imported garlic bulbs used for consumption at several traditional markets in Bogor. This finding should increase awareness of the chance of garlic contamination in Indonesia’s garlic fields. This study aimed to evaluate the nematode community and determine polyphasic characters of Aphelenchoides species from garlic plantations. The study was conducted by sampling soil and plants from garlic plantations in Tuwel and Rembul Villages, Bojong District, Tegal Regency, Central Java in December 2018. Nematode extraction was done using a mist chamber for plant materials and sugar flotation – centrifugation methods for soil samples. Parameters measured included nematode species identity, absolute population and prominence indexes. Nematode identification was conducted based on their morphological characters. Further identification based on morphometric and molecular characters (polyphasic) conducted for nematodes species suspected as quarantine pests. Six generas of nematodes identified were Helicotylenchus, Aphelenchoides, Rotylenchulus, Aphelenchus, Criconemoides, and Tylenchus. Aphelenchoides sp. had the highest prominence value from plant tissue with a value of 6.32 and is categorized as a quarantine pest genus (A. fragariae) in garlic and was further identified to the species level. Further identification based on polyphasic characters showed the Aphelenchoides sp. found was A. varicaudatus.


molecular; morphology; morphometric; prominence value

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Abawi, G.S., K. Moktan, C. Stewart, R. Hadad, & C. Hoepting. 2012. Current Status of the Bloat Nematode on Garlic in New York. Journal of Nematology 44: 447–502.

BPS (Badan Pusat Statistik). 2019. Statistik Perdagangan Luar Negeri Indonesia Impor., accessed 29/03/2019.

Carneiro, R.M.D.G., F.S.O. Lima, & V.R. Correia. 2017. Methods and Tools Currently Used for the Identification of Plant Parasitic Nematodes., accessed 27/03/2019.

Cheng, Z., K. Toyota, & K. Yamashita. 2015. Development of Real-time PCR Primers Specific to the Garlic-damaging Potato Rot Nematode Ditylenchus destructor to Quantify its Density in Soil and Outer Skin of Garlic. Nematological Research. 45: 93-99.

EPPO (European and Mediterranean Plant Protection Organization). 2013. PM 7/119 (1) Nematode Extraction. Bulletin OEPP/EPPO Bulletin 43: 471–495.

EPPO (European and Mediterranean Plant Protection Organization). 2017. PM 7/39 (2) Aphelenchoides besseyi. Bulletin OEPP/EPPO Bulletin 47: 384–400.

Firake, D.M., G.T. Behere, L. Kalita, & N.A. Thakur. 2016. Diversity Host Range and Community Analysis of Plant Parasitic Nematodes in Hill Area Ecosystems of Meghalaya. Indian Journal of Hill Farming 29: 24-28.

Fourie, H., A. McDonald, & G. Loots. 2001. Plant-parasitic Nematodes in Field Crops in South Africa. 6. Soybean. Nematology 3: 447-454.

Holterman, M., A. van der Wurff, S. van den Elsen, H. van Megen, T. Bongers, O Holovachov, J. Bakker, & J. Helder. 2006. Phylum-wide Analysis of SSU rDNA Reveals Deep Phylogenetic Relationships among Nematodes and Accelerated Evolution toward Crown Clades. Molecular Biology and Evolution 23: 1792-1800.

Huang, R.E., W. Ye, J. Liang, Q. Lu & X.Y. Zhang. 2012. Tylaphelenchus jiaae n. sp. and Aphelenchoides varicaudatus (Nematoda: Aphelenchoidinae) from Simao pine in Yunnan Province, China. Nematology 14: 93-108.

Indarti, S., S. Subandiyah, A. Wibowo, & M. Ajri. 2018. First Record: A Stem and Bulb Plant Parasitic Nematode at Garlic Area Centre Temanggung, Central Java, Indonesia with Species Reference to Ditylenchus dipsaci. Jurnal Perlindungan Tanaman Indonesia 22: 233-237.

Kementan (Kementerian Pertanian RI). 2018. Peraturan Menteri Pertanian No 31/Permentan/KR.010/7/2018 tentang Perubahan Kedua Atas Peraturan Menteri Pertanian Nomor 93/Permentan/OT.140/12/2011 tentang Jenis Organisme Pengganggu Tumbuhan Karantina. Kementerian Pertanian RI, Jakarta (ID).

Keshari, A.K., M.M. Shah, & R. Gupta. 2018. Community Analysis of Soil Nematodes in Vegetable Growing Areas of Dhading District, a High Hill of Nepal. Journal of Applied and Advanced Research 3: 156-160.

Kumar, S., G. Stecher, & K. Tamura. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution 33: 1870–1874.

Mathivathani, C. & S. Subramanian. 2018. First Report on Occurrence of Spiral Nematode, Helicotylenchus sp. in Fallow Regions of Tamil Nadu. International Journal of Agriculture Sciences 10: 6279-6280.

Norton, D.C. 1978. Ecology of Plant Parasitic Nematodes. John Wiley & Sons, Inc. New York (US), 268 p.

Reis, C.S., H. Freitas, & W. H. van der Putten. 2008. Responses of Root-feeding Nematodes (Helicotylenchus spp.) to Local and Non-local Populations of the Host Plant Ammophila arenaria. Applied Soil Ecology 39: 245-253.

Ryss, A.Y. 2017. A Simple Express Technique to Process Nematodes for Collection Slide Mounts. Journal of Nematology 49: 27-32.

Schwartz, H.F. & S.K. Mohan (eds.). 2008. Compendium of Onion and Garlic Diseases and Pests. 2nd Ed. APS Press, Minnesota (US). 127 p.

Shurtleff, M.C. & C.W. Averre, III. 2000. Diagnosing Plant Diseases Caused by Nematodes. APS Press, Minnesota (US). 189 p.

Sturhan, D. & M.W. Brzeski. 1991. Stem and Bulb Nematodes, Ditylenchus spp., p. 423-464. In W.R. Nickle (ed.), Manual of Agricultural Nematology, Marcel Decker Inc., New York (US).

Subbotin, S.A., D. Sturhan., V.N Chizhov, N. Vovlas, & J. Baldwin. 2006. Phylogenetic Analysis of Tylenchida Thorne, 1949 as Inferred from D2 and D3 Expansion Fragments of the 28S rRNA Gene Sequences. Nematology 8: 455-474.

Tarjan, A.C., R.P. Esser, & S.L. Chang. 1977. Interactive Diagnostic Key to Plant Parasitic, Freeliving and Predaceous Nematodes. Journal of the Water Pollution Control Federation 49: 2318-2337.

Van Bezooijen, J. 2006. Methods and Techniques for Nematology. Wageningen University & Research, Wageningen (NL)., 118 p, accessed 28/03/2019.


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