Disease Severity and Molecular Identification of Banana bunchy top virus, Infecting Local Banana in Bali Island

https://doi.org/10.22146/jpti.54882

Gusti Ngurah Alit Susanta Wirya(1*), I Putu Sudiarta(2), Dewa Gede Wiryangga Selangga(3)

(1) Agroecotechnology Study Program, Faculty of Agriculture, Udayana University Jln. P.B. Sudirman, Denpasar, Bali 80234
(2) Agroecotechnology Study Program, Faculty of Agriculture, Udayana University Jln. P.B. Sudirman, Denpasar, Bali 80234
(3) Agroecotechnology Study Program, Faculty of Agriculture, Udayana University Jln. P.B. Sudirman, Denpasar, Bali 80234
(*) Corresponding Author

Abstract


Bunchy top symptoms on banana has been reported in Bali Island since early 2011. Symptoms variation were observed in the field similar to infection of Banana bunchy top virus (BBTV). The identity of the BBTV in Bali on the basis of DNA-S nucleotide sequence has not been studied, therefore research was conducted to identify the species of BBTV infecting local banana in Bali based on sequence analysis. Research activities were initiated by collecting field samples from several local banana growing areas in Bali Island. Incidence of bunchy top disease in all locations reached 8% to 44% with disease severity ranged from 2.6% to 30%. Identification of BBTV from field samples were done by polymerase chain reaction using specific primers for BBTV (CPF/CPR) followed by sequence analysis of amplified DNA target. Specific BBTV DNA fragment was successfully amplified from 10 field samples; sequence analysis of DNA fragments showed their highest homology with BBTV. In addition the phylogenetic analysis confirmed the close relationship of  BBTV  isolates from Bali with various BBTV isolates from Indonesia and other isolates from the Asian group in GeneBank.


Keywords


Banana bunchy top virus; disease severity; homology sequence analysis; phylogenetic analysis; polymerase chain reaction

Full Text:

PDF


References

Amin, I., J. Qazi, S. Mansoor, M. Ilyas, & R.W. Briddon. 2008. Molecular Characterization of Banana bunchy top virus (BBTV) from Pakistan. Virus Genes 13: 191-198.

Chiaki, Y., N. Nasir, H. Herwina, Jumjunidang, A. Sonoda, T. Fukutomo, M. Nakamura, & H. Iwai. 2015. Genetic Structure and Diversity of the Banana bunchy top virus Population on Sumatra Island, Indonesia. European Journal of Plant Pathology 143: 113-122.

Dale, J.L. 1987. Banana Bunchy Top: An Economic Important Tropical Plant Virus Disease. Advances in Virus Research 33: 32-325.

Doyle, J.J. & J.J. Doyle. 1987. A Rapid DNA Isolation of Procedure for Small Quantities of Fresh Leaf Tissue. Phytochemical Bulletin 19: 11-19.

Furuya, N., S. Somowiyarjo, & K.T. Natsuaki. 2004. Virus Detection from Local Banana Cultivars and the First Molecular Characterization of Banana bunchy top virus in Indonesia. Journal of Agricultural Science 49: 75-81.

Furuya, N., T.O. Dizon, & K.T. Natsuaki. 2006. Molecular Characterization of Banana Bunchy Top Virus and Cucumber Mosaic Virus from Abaca (Musa textilis Nee). Journal of Agricultural Science 51: 92–101.

Hooks, C.R.R., M.G. Wright, D.S. Kabasawa, R. Manandhar, & R.P.P. Almeida. 2008. Effect of Banana bunchy top virus Infection on Morphology and Growth Characteristics of Banana. Annals of Applied Biology 153: 1-9.

Leiwakabessy, M., S. Nurulita, & S.H. Hidayat. 2017. Disease Incidence and Molecular Analysis of Banana bunchy top virus in Bogor, West Java. In D. Efendi & A. Maharijaya (eds), The Future of Tropical Horticulture. International Proceeding on Tropical Horticulture 2016: The Future of Tropical Horticulture. Bogor, Indonesia, November 28-29, 2016.

Mansoor, S., J. Qazi, I. Amin, A. Khatri, I.A. Khan, S. Raza, Y. Zafar, & R.W. Bridon. 2005. A PCR-based Method with Internal Control for the Detection of Banana bunchy top virus in Banana. Molecular Biotechnology 30: 127-129.

Niyongere, C., T. Losenge, E.M. Ateka, D. Nkezabahizi, G. Blomme, & P. Lepoint. 2011. Occurrence and Distribution of Banana Bunchy Top Disease in the Great Lakes Region of Africa. Tree and Forestry Science and Biotechnology 6: 102-107.

Pinili, M.S., D.N. Nyana, G. Suastika, & K.T. Natsuaki. 2011. Molecular Analysis of Banana bunchy top virus First Isolated in Bali, Indonesia. Journal of Agricultural Science 56: 125-134.

Rahim, Y.F., A.D. Tri, & G. Munif. 2015. Detection of Viruses that Infect Soybeans in West Java. Jurnal Fitopatologi Indonesia 11: 59-67.

Statistics of Bali Province. 2019. Banana Fruit Production Specified by Regency / City in Bali, 2000-2017. Available via DIALOG. https://bali.bps.go.id/dynamictable/2017/05/18/129/produksi-buah-pisang-dirinci-menurut-kabupaten-kota-di-bali-2000-2017.html. modified 28/05/2019.

Thomas, J.E. & M.L. Iskra-Caruana. 2000. Bunchy Top, p. 241–253. In R.D. Jones (ed), Diseases of Banana, Abaca and Ensete. CAB International, Wallingford.

Vetten, H.J., P.W. Chu, J.L. Dale, R.M. Harding, J. Hu, L. Katul, M. Kojima, J.W. Randles, Y. Sano, & J.E. Thomas. 2005. Nanoviridae, p. 343–352. In C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball (eds.), Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses. Academic Press, San Diego.



DOI: https://doi.org/10.22146/jpti.54882

Article Metrics

Abstract views : 681 | views : 137

Refbacks

  • There are currently no refbacks.




Copyright (c) 2020 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 height=auto

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print)ISSN 2548-4788 (online) Accredited Journal, Based on Decree of the Minister of Research, Technology and Higher Education, Republic of Indonesia Number 30/E/KPT/2018, Vol. 21 No. 1 the Year 2017 - Vol. 25 No. 1 the Year 2021Indexing & Abstracting , 

View My Stats