The Existence of Papaya ringspot virus-Papaya Strain on Cucumber in Gianyar, Bali
Dewa Gede Wiryangga Selangga(1), I Ketut Widnyana(2), Listihani Listihani(3*)
(1) Faculty of Agriculture, Warmadewa University Jln. Terompong No. 24, Tanjung Bungkak, Denpasar, Bali 80239 Indonesia
(2) Faculty of Agriculture and Business, University of Mahasaraswati Denpasar Jln. Kamboja No. 11A, Dangin Puri Kangin, Denpasar Utara, Bali 80233 Indonesia
(3) Faculty of Agriculture and Business, University of Mahasaraswati Denpasar Jln. Kamboja No. 11A, Dangin Puri Kangin, Denpasar Utara, Bali 80233 Indonesia
(*) Corresponding Author
Abstract
Yellow mosaic symptoms were identified from cucumber production systems in Gianyar and were similar to symptoms of PRSV infection. Further research was conducted to determine diseases incidence and molecular characteristic of PRSV. Ninety leaf samples were collected from Gianyar by purposive sampling and disease incidence calculations were based on symptoms in the field. Detection and identification were done using a RT-PCR with specific primers of CP PRSV-P, CP PRSV-W and DNA sequencing. Disease incidences in the fields ranged between 5.81–66.87%. Specific DNA band 470 bp was successfully amplified from several cucumber leaf samples collected from Ubud, Payangan, Tegallalang, Sukawati, Gianyar, and Blahbatuh; but no DNA were amplified from all samples when using CP PRSV-W specific primer. Nucleotide and amino acid analysis showed nucleotides homology to isolates from Ubud, Payangan, Tegallalang, Sukawati, Gianyar, and Blahbatuh, i.e. 98.9–99.5% and 99.1–100%, respectively. Results indicated that genetic variation of PRSV-P from Gianyar was low. Furthermore, the nucleotides homology of PRSV-P isolates from Ubud, Payangan, Tegallalang, Sukawati, Gianyar, and Blahbatuh were with PRSV-P isolates which infected cucumbers from Nganjuk (LC311783) and Brebes (LC311784), while from native papaya collected in Bali Bali (LC223115) were 97.2–98.4% and 98.6–100%, respectively. Phylogenetic analysis confirmed that PRSV-P isolates from Indonesia were in the same cluster with Thailand isolates. The results showed that sources of PRSV-P inoculums spreading into new areas.
Keywords
Full Text:
PDFReferences
Bateson, M.F., Lines, R.E., Revill, P., Chaleeprom, W., Ha, C.V., Gibbs, A.J., & Dale, J.L. (2002). On the Evolution and Molecular Epidemiology of the Potyvirus Papaya ringspot virus. Journal of General Virology, 83(10), 2575–2585. https://doi.org/10.1099/0022-1317-83-10-2575
Bayot, R.G., Villegas, V.N., Magdalita, P.M., Jovellana, M.D., Espino, T.M., & Exconde, S.B. (1990). Seed Transmissibility of Papaya ringspot virus. Philippines Journal of Crop Science, 15(2), 107–111. Retrieved from https://www.cabi.org/isc/FullTextPDF/pre2000/19932331431.pdf
Davis, R.I., Mu, L., Maireroa, N., Wigmore, W.J., Grisoni, M., Bateson, M.F., & Thomas, J.E. (2005). First Records of the Papaya Strain of Papaya ringspot virus (PRSV-P) in French Polynesia and the Cook Islands. Australasian Plant Pathology, 34(1), 125–126. https://doi.org/10.1071/AP04094
Doyle, J.J., & Doyle, J.L. (1987). A Rapid DNA Isolation Procedure for Small Quantities of Fresh Leaf Tissue. Phytochemical Bulletin, 19(1), 11–15. https://doi.org/ 10.12691/jfnr-2-7-6
Gonsalves, D., Tripathi, S., Carr, J.B., & Suzuki, J.Y. (2010). Papaya ringspot virus. Plant Health Instructor, 149, 2435–2442. https://doi.org/10.1094/PHI-I-2010-1004-01
Harmiyati, T., Hidayat, S.H., & Adnan, A.M. (2015). Deteksi dan Respons Lima Varietas Pepaya terhadap Tiga Isolat Papaya ringspot virus (PRSV). Jurnal Agro Biogen, 11(3), 87–94. https://doi.org/10.21082/jbio.v11n3.2015.p87-94
Hidayat, S.H., Nurulita, S., & Wiyono, S. (2012). Temuan Penyakit Baru Infeksi Papaya ringspot virus pada Tanaman Pepaya di Nangroe Aceh Darussalam. Jurnal Fitopatologi Indonesia, 8(6), 184–187. https://doi.org/10.14692/jfi.8.6.184
Kalleshwaraswamy, C.M. & Kumar, N.K. (2008). Transmission Efficiency of Papaya ringspot virus by Three Aphid Species. Phytopathology, 98(5), 541–546. https://doi.org/10.1094/PHYTO-98-5-0541
Laili, N.U. & Damayanti, T.A. (2019). Deteksi Virus pada Tanaman Mentimun di Jawa Barat. Agrovigor, 12(1), 8–15. https://doi.org/10.21107/agrovigor.v12i1.5089
Listihani, Damayanti, T.A., Hidayat, S.H., & Wiyono, S. (2018). Karakterisasi Molekuler Papaya ringspot virus tipe P pada Tanaman Mentimun di Jawa. Jurnal Fitopatologi Indonesia 14(3), 75–82. https://doi.org/10.14692/jfi.14.3.75
Mansilla, P.J., Moreira, A.G., Mello, A.P.O.A., Rezende, J.A.M., Ventura, J.A., Yuki, V.A., & Levatti, F.J. (2013). Importance of Cucurbits in the Epidemiology of Papaya ringspot virus type P. Plant Pathology, 62(3), 571–577. https://doi.org/10.1111/j.1365-3059.2012.02677.x
Martinez, D.R., Duarte, P.D.S.G., Olmedo, J.G., & Figueira, A.D.R. (2014). Molecular and Biological Studies of Papaya ringspot virus Isolates from Brazil and Cuba. American Journal of Agriculture and Forestry, 2(5), 209–218. https://doi.org/10.11648/j.ajaf.20140205.11
Martinez, D.R., Figuera, A.R., Duarte, P.S.G., Costa, S.B.F.G., & Olmedo, J.G. (2015). First Report and Molecular Characterization of an Isolate of Papaya ringspot virus (PRSV-W) Detected in Pumpkin in Cuba. Bioscience Journal, 31(4), 1133–1142. https://doi.org/10.14393/BJ-v31n4a2015-26181
Mohammed, H., Manglli, A., Zicca, S., Mohammed, M., & Tomassoli, L. (2012). First Report of Papaya ringspot virus in Pumpkin in Sudan. New Disease Reports, 26(1), 26–33. http://doi.org/10.5197/j.2044-0588.2012.026.026
Nurhantoro, I., Hidayat, S.H., & Mutaqin, K.H. (2018). Penggunaan Pelacak DNA untuk Deteksi Papaya ringspot virus dengan Metode Hibridisasi Asam Nukleat. Jurnal Fitopatologi Indonesia, 14(3), 89–96. https://doi.org/10.14692/jfi.14.3.89
Singh, S., Awasthi, L.P., Kumar, P., & Jagre, A. (2017). Diagnostic Characteristics of Papaya ringspot virus Isolates Infecting Papaya (Carica papaya L.) in India. JOJ Immuno Virology, 1(4), 555567. https://doi.org/10.19080/009 JOJIV.2017.01.555567
Tennant, P.F., Fermin, G.A., & Roye, M.E. (2007). Viruses Infecting Papaya (Carica papaya): Etiology, Pathogenesis, and Molecular Biology. Plant Viruses, 1(2), 178–188. Retrieved from https://www.globalsciencebooks.info/Online/GSBOnline/images/0712/PV_1(2)/PV_1(2)178-188o.pdf
Thomas, J.E. & Dodman, R.L. (1993). The First Record of Papaya ringspot virus-type P from Australia. Australasian Plant Pathology, 22(1), 2–7. https://doi.org/10.1071/APP9930002
Zhu, L., Yang, T., Chen, L.J., Lin, H.H., & Xi, D.H. (2016). First Report of Papaya ringspot virus Infecting Bitter Gourd in China. Plant Disease, 100(11), 2338. https://doi.org/10.1094/PDIS-01-16-0086-PDN
DOI: https://doi.org/10.22146/jpti.64703
Article Metrics
Abstract views : 2154 | views : 1408Refbacks
- There are currently no refbacks.
Copyright (c) 2021 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics