Isolation, Characterization, and Selection of Bacillus sp. from Shallot Rhizosphere that Inhibits Fusarium oxysporum Growth

https://doi.org/10.22146/jpti.89634

Alil Saputra(1), Nur Prihatiningsih(2*), Heru Adi Djatmiko(3), Dhadhang Wahyu Kurniawan(4)

(1) Department of Agrotechnology, Faculty of Agriculture, Universitas Jenderal Soedirman Jln. DR. Soeparno No. 61, Purwokerto, Central Java 53123 Indonesia
(2) Department of Agrotechnology, Faculty of Agriculture, Universitas Jenderal Soedirman Jln. DR. Soeparno No. 61, Purwokerto, Central Java 53123 Indonesia
(3) Department of Agrotechnology, Faculty of Agriculture, Universitas Jenderal Soedirman Jln. DR. Soeparno No. 61, Purwokerto, Central Java 53123 Indonesia
(4) Department of Pharmacy, Faculty of Health Sciences, Universitas Jenderal Soedirman Jln. DR. Soeparno No. 63 Purwokerto, Central Java 53122 Indonesia
(*) Corresponding Author

Abstract


Bacillus sp. is a Plant Growth Promoting Rhizobacteria (PGPR) species that lives in the rhizosphere. This bacterium can produce antifungal compounds that suppress pathogenic fungi, such as Fusarium oxysporum. The aim of this research was isolate, characterize, and obtain Bacillus sp. that can inhibit F. oxysporum which causes twisted disease in shallots. Isolation was done by sampling 10 g of soil from shallot roots, placing it in 9 mL of sterile water, and carrying out a series of dilutions. The first dilution was incubated at 80 °C for 20 minutes to obtain Bacillus sp. on the selective medium HiChrome. Colony morphology, shape, cell color, Gram staining, catalase and endospore staining were observed from obtained isolates. An antagonist test was done to determine the inhibitory effects of isolates against the pathogenic fungus F. oxysporum. Four isolates of Bacillus sp. were obtained with irregular colony shape, dull white color without muccus, edges varying in shape between lobate and undulate, and raised elevation. The results of the antagonist test showed that the four isolates had the ability to inhibit the growth of the pathogenic fungus F. oxysporum with the greatest inhibition shown by isolate BM1 at 30.12%, with an antibiosis mechanism and hyphae swelling.


Keywords


Bacillus; dual culture; Fusarium oxysporum; shallot

Full Text:

PDF


References

Ainy, E.Q., Ratnayani, R., & Susilawati, L. (2015). Uji Aktivitas Antagonis Trichoderma harzianum 11035 terhadap Colletotrichum capsici TCKR2 dan Colletotrichum acutatum TCK1 Penyebab Antraknosa pada Tanaman Cabai. Prosiding Seminar Nasional XII Pendidikan Biologi FKIP UNS, 12(1), 892‒897. Retrieved from https://jurnal.uns.ac.id/prosbi/article/view/7122/6350

Bais, H.P., Fall, R., & Vivanco, J.M. (2004). Biocontrol of Bacillus subtilis against Infection of Arabidopsis Roots by Pseudomonas syringae Is Facilitated by Biofilm Formation and Surfactin Production. Plant Physiology, 134(1), 307‒319. https://doi.org/10.1104/pp.103.028712

Dinata, G.F., Ariani, N., Purnomo, A., & Aini, L.Q. (2021). Pemanfaatan Biodiversitas Bakteri Serasah Kopi Sebagai Solusi Pengendali Penyakit Moler pada Bawang Merah. Jurnal HPT (Hama Penyakit Tumbuhan), 9(1), 28‒34. https://doi.org/10.21776/ub.jurnalhpt.2021.009.1.5

Deden, & Umiyati, U. (2017). Pengaruh Inokulasi Trichoderma sp. dan Varietas Bawang Merah terhadap Penyakit Moler dan Hasil Tanaman Bawang Merah (Allium ascalonicum L.) [The Effect of Trichoderma sp. Inoculation and Shallot “Bawang merah” Variety on Moler Diseases and Yield of Shallot]. Jurnal Kultivasi, 16(2), 340‒348. Retrieved from https://jurnal.unpad.ac.id/kultivasi/article/view/12213/0

Ismy, A., Syauqi, A., & Zayadi, H. (2019). Keanekaragaman Koloni Mikroorganisme Rizosfer Lahan Tebu (Saccharum officinarum) pada Penggunaan Pupuk Bio-Slurry dan Pupuk Kimia [Colony Diversity of Rizosphere Microorganisms of Sugarcane (Saccharrum officinarum) Field on Bio-Slurry and Chemical Fertilizers Utilization]. Jurnal Ilmiah Biosaintropis (Bioscience-Tropic), 5(1), 25‒30. Retrieved from https://biosaintropis.unisma.ac.id/index.php/biosaintropis/article/view/223

Jo, J., Subroto, S.T., Victor, H., & Sanjaya, A. (2021). Pengujian Aktivitas Antijamur Bacillus amyloliquefaciens strain n1 [Assessment on Antifungal Activity of Bacillus amyloliquefaciens strain n1]. FaST-Jurnal Sains dan Teknologi (Journal of Science and Technology), 5(2), 126‒133. Retrieved from https://ojs.uph.edu/index.php/FaSTJST/article/view/4681/0

Kavitha, T.R., Suneetha, C., & Sunitha, T.R. (2022). Heat-Treatment Method for Isolation of Bacillus spp. from Plant Tissues. In A. Sankaranarayanan, N. Amaresan, M.K. Dwivedi (Eds.), Endophytic Microbes: Isolation, Identification, and Bioactive Potentials (pp. 7‒13). New York, NY: Springer US. https://link.springer.com/protocol/10.1007/978-1-0716-2827-0_2

Lestari, P., Prihatiningsih, N., & Djatmiko, H.A. (2017). Partial Biochemical Characterization of Crude Extract Extracellular Chitinase Enzyme from Bacillus subtilis B 298. IOP Conference Series: Materials Science and Engineering, 172, 012041. https://doi.org/10.1088/1757-899X/172/1/012041

Moreira, R.R., Nesi C.N., & De Mio, L.L.M. (2014). Bacillus spp. and Pseudomonas putida as Inhibitors of the Colletotrichum acutatum Group and Potential to Control Glomerella Leaf Spot. Biological Control, 72, 30‒37. https://doi.org/10.1016/j.biocontrol.2014.02.001

Mukamto, Ulfa, S., Mahalina, W., Syauqi, A., Istiqfaroh, L., & Trimulyono, G. (2015). Isolasi dan Karakterisasi Bacillus sp. Pelarut Fosfat dari Rhizosfer Tanaman Leguminosae [Isolation and Characterization of Phosphate Solubizing Bacteria Bacillus sp. from the Rhizosphere of Leguminosae Plants]. Sains dan Matematika, 3(2), 62‒68. Retrieved from https://journal.unesa.ac.id/index.php/sainsmatematika/article/view/221

Nadarajah, K. & Ali, H.Z. (2015). Trichoderma and Bacillus as Biocontrol Agents against Fusarium in Rice. Journal of Pure and Applied Microbiology, 9(4), 3245‒3255. Retrieved from https://microbiologyjournal.org/trichoderma-and-bacillus-as-biocontrol-agents-against-fusarium-in-rice/

Pendyala, B., Patras, A., Gopisetty, V.V.S., Sasges, M., & Balamurugan, S. (2019). Inactivation of Bacillus and Clostridium Spores in Coconut Water by Ultraviolet Light. Foodborne Pathogens and Disease, 16(10), 704‒711. https://doi.org/10.1089/fpd.2019.2623

Prihatiningsih, N., Djatmiko, H.A., & Lestari, P. (2017). Aktivitas Siderofor Bacillus subtilis sebagai Pemacu Pertumbuhan dan Pengendali Patogen Tanaman Terung [Siderophore Activity of Bacillus subtilis as Plant Growth Promoters and Biological Control Agent of Eggplants Pathogens]. Jurnal Hama dan Penyakit Tumbuhan Tropika, 17(2), 170‒178. Retrieved from https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/420

Prihatiningsih, N., Djatmiko, H.A., Erminawati, & Lestari, P. (2019). Bacillus subtilis from Potato Rhizosphere as Biological Control Agent and Chili Growth Promoter. Jurnal Perlindungan Tanaman Indonesia, 23(2), 179–184. https://doi.org/10.22146/jpti.40606

Prihatiningsih, N., Arwiyanto, T., Hadisutrisno, B., & Widada, J. (2020). Characterization of Bacillus spp. from the Rhizosphere of Potato Granola Variety as an Antibacterial against Ralstonia solanacearum. BIODIVERSITAS, 21(9), 4199–4204. https://doi.org/10.13057/biodiv/d210934

Prihatiningsih, N., Djatmiko, H.A., & Lestari, P. (2022). Antagonistic Feature Displayed by Endophytic Bacteria Consortium for Control Rice Pathogens. Journal of Tropical Plant Pests and Diseases, 22(2), 154–161. Retrieved from https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/657

Prihatiningsih, N., Djatmiko, H.A., & Lestari, P. (2023). Metode Isolasi Bakteri Endofit Akar Padi Khusus Bacillus sp. Paten No. IDS000005984

Rahayuniati, R.F., & Mugiastuti, E. (2012). Keefektifan Bacillus sp. dan Pseudomonas fluorescens Mengendalikan Fusarium oxysporum f. sp. lycopersici dan Meloidogyne sp. Penyebab Penyakit Layu pada Tomat secara In Vitro. Jurnal Pembangunan Pedesaan, 12(1), 65–70.

Saxena, A.K., Kumar, M., Chakdar, H., Anuroopa, N., & Bagyaraj, D.J. (2020). Bacillus species in Soil as a Natural Resource for Plant Health and Nutrition. Journal of Applied Microbiology, 128(6), 1583–1594. https://doi.org/10.1111/jam.14506

Supriatna, S., Siahaan, S., & Restiaty, I. (2021). Pencemaran Tanah oleh Pestisida di Perkebunan Sayur Kelurahan Eka Jaya Kecamatan Jambi Selatan Kota Jambi (Studi Keberadaan Jamur Makroza dan Cacing Tanah). Jurnal Ilmiah Universitas Batanghari Jambi, 21(1), 460–466. http://doi.org/10.33087/jiubj.v21i1.1348

Triwidodo, H., & Tanjung, M.H. (2020). Hama Penyakit Utama Tanaman Bawang Merah (Allium ascalonicum) dan Tindakan Pengendalian di Brebes, Jawa Tengah [Shallot (Allium ascalonicum) Pests and its Control Measures in Brebes, Central Java]. Agrovigor: Jurnal Agroekoteknologi, 13(2), 149–154. https://doi.org/10.21107/agrovigor.v13i2.7131

Wang, H., Wen, K., Zhao, X., Wang, X., Li, A., & Hong, H. (2009). The Inhibitory Activity of Endophytic Bacillus sp. strain CHM1 against Plant Pathogenic Fungi and its Plant Growth-promoting Effect. Crop Protection, 28(8), 634–639. https://doi.org/10.1016/j.cropro.2009.03.017

Widiantini, F., Yulia, E., & Kurniawan, A. (2020). Penghambatan Pertumbuhan Rhizoctonia oryzae dan Cercospora oryzae oleh Senyawa Volatil yang Dihasilkan Bakteri Endofit Padi [Inhibiton Growth of Rhizoctonia oryzae and Cercospora oryzae by Volatile Compound Produced by Rice Endophytic Bacteria]. Jurnal Agrikultura, 31(1), 61–67. https://doi.org/10.24198/agrikultura.v31i1.27323

Wulandari, N., Irfan, M., & Saragih, R. (2019). Isolasi dan Karakterisasi Plant Growth Promoting Rhizobacteria dari Rizosfer Kebun Karet Rakyat [Isolation and Characterization of Plant Growth Promoting Rhizobacteria from the Rizosphere of Folk Rubber Plantations]. Dinamika Pertanian, 35(3), 57–64. https://doi.org/10.25299/dp.2019.vol35(3).4565

Wulansari, N.K., Prihatiningsih, N., & Djatmiko, H.A. (2018) tahun dicek ulang 2017. Mekanisme Antagonis Lima Isolat Bacillus subtilis terhadap Colletotrichum capsici dan C. gloeospoiroides In Vitro [Antagonistic of Five Isolates of Bacillus subtilis to Colletotrichum capsici and Colletotrichum gloeospoiroides In Vitro]. Agrin, 21(2), 127–139. http://doi.org/10.20884/1.agrin.2017.21.2.371

Yuliana, N., Sarkono, Hidayati, E., & Faturrahman. (2022). Isolasi, Karakterisasi, dan Identifikasi Bacillus spp. Berasosiasi Abalon (Haliotis asinina) [Isolation, Characterization and Identification of Bacillus spp. Associated with Abalone (Haliotis asinina)]. Samota Journal of Biological Science, 1(1), 1–10. Retrieved from https://journal.unram.ac.id/index.php/samota/article/view/1322



DOI: https://doi.org/10.22146/jpti.89634

Article Metrics

Abstract views : 317 | views : 135

Refbacks

  • There are currently no refbacks.




Copyright (c) 2024 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics