Pengendalian Nematoda Parasitik Tanaman secara Hayati dengan Bakteri Pasteuria penetrans: Inventarisasi, Pembiakan Massal, dan Uji Patogenisitas Isolat Bakteri

Mulyadi Mulyadi(1*), B. Triman(2), Bambang Rahayu TP(3)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author


The research on “Biological control of plant parasitic nematodes by nematophagous bacteria (Pasteuria penetrans)” was planned to be conducted within 3 years period, started in 1994/1995. In the first year research was done with the following objectives: 1) inventory on the isolates of P. penetrans, and 2) study on the mass production and pathogenicity test of P. penetrans. Survey of P. penetrans was done in the provinces of D.I. Yogyakarta, Central Java, East Java, and West Java. Soil and root samples were collected in this survey, plant parasitic nematodes were isolated using centrifugation and funnel and spray method. Mass production of P. penetrans was done with their host especially root-knot nematodes (Meloidogyne spp.). The bacterial infected nematodes were inoculated on tomato  planted on sterilized soil. Roots contain root-knot nematodes inhabiting spores of P. penetrans were used as inoculum source. The pathogenicity tests of the isolates were done using bioassay method and in the green house in Completely Randomized Design.The research results is as follows: 1) seventeesn isolates of P. penetrans were found in the survey, these isolates were pathogenic to root-knot nematodes, 2) mass production of P. penetrans was done with their host especially root-knot nematodes and 4) treatment with P. penetrans significantly reduced the root-gall caused by root-knot nematodes.


biological control; parasitic nematodes; Pasteuria penetrans

Full Text:



Bernard, E.C. 1994. Nematology in the 21 St Century: A foray into the future. Phytopathol. News 28 :40-41.

Bird, A.F. and P.G. Brisbane. 1988. The influence of Posteuria penetrans in field soils on the reproduction of root-knot nematodes. Revue Nematol. 11: 75-81.

Dropkin, V.H. 1988. Introduction of plant nematology. A Wiley lnterscience Publication. John Wiley and Sons, New York, 305 p.

Khan, M.W. dan M.N. Esfahani. 1992. Root-knot of vegetables. Dalam H.S. Chaube; J. Kumar; A.N. Mukhopadhay and U.S. Singh (Ed.) Plant diseases of international importance. Prentice Hall, New Yersey, 376 p.

Luc, M., R..A. Sikora and J. Bridge. 1990. Plant parasitic nematodes in subtropical and tropical agriculture. C.A.B. Int. Inst. of Parasitol. 629 p.

Mai, W.F. 1985. Plant parasitic nematodes their threat to agriculture. Dalam J.N. Sasser dan C.C. Carter (Ed.) An advanced treatise on Meloidogyne, vol. I. Biology and control. North Carolina State Univ. Graph. p. 11-17.

Mankau, R. 1980. Biological control of nematodes pests by natural enemies. Ann. Rev. Phytopathol. 18 :415-440.

Mulyadi, B. Triman dan C. Netscher. 1992. Pemanfaatan bakteri Pasteuria penetrans untuk mengendalikan nematoda parastik tanaman secara hayati. Fak. Pertanian UGM. 31p.

Sayre, R.M. 1988. Bacterial disease of nematodes and their role in controlling nematode populations. Dalam Edwards, C.A, D. Stinner and S. Rabatin (Ed.), Biological interaction in soil. Elsevier, Tokyo, p. 263-279.

Stirling, G.R 1984. Biological control of Meloidogyne javanica with Bacillus penetrans. Phytopathology, 74 : 55-60.

Stirling, G.R. dan A.M. White. 1982. Distribution of a parasite of root-knot nernatode in South Australian vineyards. Plant Disease 66:52-53.

Sturhan, D. 1988. New host and geographical records of nematode parasitic bacteria of the Pasteuria penetrans group. Nematologica 34: 350-356.

Zeck, W.M. 1970. A rating scheme for field evaluation of root-knot nematodes infestation. Bayer 24: 141-144.


Article Metrics

Abstract views : 2453 | views : 1226


  • There are currently no refbacks.

Copyright (c) 1995 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print)ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics