Isolasi Bakteri Inti Es pada Kentang

https://doi.org/10.22146/jpti.9360

Triwidodo Arwiyanto(1*)

(1) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Frost injury is one of the limiting factors in potato production in Dieng (2000 m above sea level), Central Java. The damage caused by frost has been recorded since long time ago, however, there is no studies about this matter. The objective of the present study is to isolate ice nucleation active bacteria which reside epiphytically at the surface of potato leaves. Potato and several crop leaves were collected and the ice catalyst was detected from leaf-washing. The bacteria were isolated on Nutrien Agar and Nutrien Agar Glycerol medium. The result indicated that ice catalyst was detected from the leaf washing of potato (Solanum tuberosum L.) and broad bean (Vicia faba L.) but not from others.  The ice catalyst was not detected from the extract of surface soil, neither. The ice nucleation active bacteria were successfully isolated from potato leaf and were identical to Erwinia ananas in their bacteriological properties.

 


Keywords


ice nucleating-active bacteria; potato; frost

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References

Bradbury, J.F. 1986. Guide to Plant Pathogenic Baciena. CMI. 332pp.

Goto, K.; T. Inaba and M. Goto. 1988. Factors affecting frost damage of vegetable crops by artificial spraying of ice nucleation-active bacteria in a freezing chamber. Proc. Assoc. Pl. Protec. Shikoku 23: 47-55. In Japanese.

Goto, M.; T. Goto and T. Inaba. 1989. Identification of Ice Nucleation-Active Bacteria Isolated from Frost Damaged Vegetable Leaves. Ann. Pytopath. Soc. Japan 55:330-335.

Hayward, AC. 1990. Proposals for a Quick Practical Identification. In: Klement. Z.: K. Rudolph and D.C. Sands (eds.) Methods in Phytobacteriology. p 269-274. Akademiai Kiado. Budapest.

Kricg, N.R. and J.G. Holt. 1984. Bergey’s Manual of .Systematic Bacteriology. Vol 1. William and Wilkins. Baltimore. 964 pp.

Lindow, S.E. 1983. The Role of Bacterial Ice Nucleation in Frost Injury to Plants. Ann Rev. Phytopathol. 21: 363-384.

Lindow, S.E.; Arny, D.C. and Upper, C.D. 1978. Distribution of Ice Nucleation-Active Bacteria on Plants in Nature. App!. Environ. Microbial. 36: 831-838.

Sands, D.C. 1990. Physiological Criteria-Determinative Tests. In: Klement, Z.; K. Rudolph and D.C. Sands (eds.) Methods in Phytobacteriology. p 134-152. Akademiai Kiado. Budapest.

Semangun, H. 1996. Pengantar Ilmu Penyakit Tumbuhan. Gadjah Mada University Press. 754 pp.

Semangun, H. 1979. Penyakit Tumbuhan, Hubungannya dengan Iklim dan Cuaca. Simposium Meteorologi Pertanian, Bogor, 3-8 Maret 1979. Tidak dipublikasikan.



DOI: https://doi.org/10.22146/jpti.9360

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Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

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