* Corresponding Author
Effect of Different Growing Media on the Growth of Cellulolytic Bacteria from Oryctes rhinoceros Larva
https://doi.org/10.22146/jpti.99959 Andreas Sitepu(1), Marheni Marheni(2*), Suzanna Fitriany Sitepu(3)
(1) Department of Agrotechnology, Faculty of Agriculture, Universitas Sumatera Utara Jln. Dr. A. Sofian No. 3, Padang Bulan, Medan Baru, Medan, North Sumatra 20155 Indonesia
(2) Department of Agrotechnology, Faculty of Agriculture, Universitas Sumatera Utara Jln. Dr. A. Sofian No. 3, Padang Bulan, Medan Baru, Medan, North Sumatra 20155 Indonesia
(3) Department of Agrotechnology, Faculty of Agriculture, Universitas Sumatera Utara Jln. Dr. A. Sofian No. 3, Padang Bulan, Medan Baru, Medan, North Sumatra 20155 Indonesia
(*) Corresponding Author
Abstract
Oryctes rhinoceros larvae are one of the sources of cellulose and hemicellulose degrading bacteria. Nutrition and energy sources together, with environmental factors have significant impacts on cellulolytic bacteria growth. Carboxymethyl Cellulose (CMC) media is a media often used to obtain cellulosic bacteria, while Nutrient Agar (NA) media is used for bacteria propagation. The use of NA for bacterial growth media is expensive. For the growth of O. rhinoceros symbiont cellulolytic bacteria, it is necessary to use alternative media that are cheap, easily available and contain sufficient nutrients to sustain bacterial growth. Soybeans, mackerel tuna, and jackfruit seeds contain high levels of protein and carbohydrate, which can be used as a nitrogen and carbon source for cellulolytic bacteria growth are easily available and cheap. This makes them excellent alternative for growth media materials. The aim of this research was to determine growth of symbiotic cellulolytic bacteria from O. rhinoceroson different alternative growth media. The research was conducted from January to May 2024 at the Plant Disease Laboratory, Faculty of Agriculture and Microbiology Laboratory, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara. The experimental method used a factorial Completely Randomized Design (CRD) with 2 factors and 3 replications for each treatment combinations. The results showed that alternative growth media were suitable to sustain cellulolytic bacteria. The best treatment was mackerel tuna flour media with an average number of colonies of 100.5×106CFU/mL.
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Al-Ayubi, M.S., Pestariati, P., Anggraini, A.D., & Mutiarawati, D.T. (2022). Potensi Ikan Tongkol dan Ikan Lele sebagai Media Alternatif Pertumbuhan Escherichia coli. The Journal of Muhammadiyah Medical Laboratory Technologist, 5(2), 124–131. https://doi.org/10.30651/jmlt.v5i2.14407
Asri, A., Sakinah, A., & Mauboy, R.S. (2019). Penggunaan Media Tepung Limbah Ikan Cakalang Untuk Pertumbuhan Bakteri Eschericia coli dan Staphycoccus aureus. Jurnal Biotropikal Sains, 16(3), 36–46. Retrieved from https://ejurnal.undana.ac.id/index.php/biotropikal/article/view/1726/1323
Azizah, R., & Antarti, A.N. (2019) Uji Aktivitas Anti Bakteri Ekstrak dan Getah Pelepah serta Bonggol Pisang Kepok Kuning (Musa paradisiaca Linn.) terhadap Bakteri Pseudomonas aeruginosa dan Klebsiella pneumoniae dengan Metode Difusi Agar [Activity of Extract and Saps from Midrib and Stem’s Musa parasidiaca Linn. against Pseudomonas aeruginosa and Klebsiella pneumoniae Bacteria using Agar Diffusion Method]. JPSCR Journal of Pharmaceutical Science and Clinical Research, 4(1), 29–38. https://doi.org/10.20961/jpscr.v4i1.26544
Basalamah, N.A., Nurlaelah, I., & Handayani, H. (2018). Pengaruh Subtitusi Ekstrak Kedelai terhadap Karakteristik Selulosa Bakteri Acetobacter xylinum dalam Pembuatan Nata De Sweet Potato. Quagga: Jurnal Pendidikan dan Biologi, 10(1), 24–31. https://doi.org/10.25134/quagga.v10i01.805
Choi, Y.W., Hodgkiss, I.J., & Hyde, K.D. (2005). Enzyme Production by Endophytes of Brucea javanica. Journal of Agricultural Technology, 1, 55–66.
Cilia., Muskita, W.H., & Kurnia, A. (2016). Pengaruh Penggunaan Tepung Ikan Layang (Decapterus russelli) dengan Tepung Ikan Tongkol (Euthynnus affinis) dalam Pakan terhadap Pertumbuhan dan Tingkat Kelangsungan Hidup Juvenil Udang Vaname (Litopenaeus vannamei) [The Effect of Flying Fish Meal (Decapterus russelli) and Tuna Fish Meal (Euthynnus affinis) in Fed on Growth and Survival Rate of White Shrimp Juvenile (Litopenaeus vannamei)]. Media Akuatika, 1(4), 177–186. Retrieved from https://ojs.uho.ac.id/index.php/JMA/article/view/4293
Dillon, R., & Dillon, V. (2004). The Gut Bacteria of Insects: Nonpathogenic Interactions. Annual Review of Entomology, 49(1), 71–92. https://doi.org/10.1146/annurev.ento.49.061802.123416
Dini, I.R., Wawan, W., Hapsoh, H., & Sriwahyuni, S. (2018). Isolation and Identification of Cellulolytic and Lignolytic Bacteria from the Gut Oryctes rhinoceros L. Larvae Decomposition of Oil Palm Empty Fruit Bunches. Indonesian Journal of Agricultural Research, 1(2), 193–203. https://doi.org/10.32734/injar.v1i2.314
Fahruddin, F. (2018). Analisis Populasi Bakteri pada Air Asam Tambang Dengan Perlakuan Sedimen Mangrove [Population Analysis of Bacteria on Acid Mine Water with Mangrove Sediment Treatment]. Jurnal Ilmu Alam dan Lingkungan, 9(1), 70–77. Retrieved from https://journal.unhas.ac.id/index.php/jai2/article/view/4875
Hasibuan, H.B., Marheni, N., & Rauf, A. (2021). Role of Bacterial Symbionts of Larvae Oryctes rhinoceros L. and Microbial Decomposer on Composting of Empty Bunch of Oil Palm in Big Hole Planting System. AIP Conference Proceedings, 2342, 070002. https://doi.org/10.1063/5.0046531
Hasibuan, N.H., Hutapea, S., & Rahman, A. (2022). Pemanfaatan Limbah Kulit Jengkol sebagai Bahan Baku Kompos dan Biochar dalam Meningkatkan Pertumbuhan dan Produksi Tanaman Bawang Merah (Allium ascalonicum L.) [Utilization of Jengkol Skin Waste as Raw Material for Compost and Biochar in Increasing Growth and Production of Shallots (Allium ascalonicum L.). Jurnal Ilmiah Pertanian (JIPERTA), 4(1), 32–44. https://doi.org/10.31289/jiperta.v4i1.1193
Hidayat, R., Maimun, M., & Sukarno, S. (2020). Analisis Mutu Pindang Ikan Tongkol (Euthynnus affinis) dengan Teknik Pengolahan Oven Steam. Jurnal FishtecH, 9(1), 21–33. https://doi.org/10.36706/fishtech.v9i1.11003
Indrayati, S., Utami, P.R., & Oktaviani, I.R. (2021). Pemanfaatan Serbuk Kacang Kedelai (Glycine max L. Merr) sebagai Bahan Pengganti Beef Extract pada Media Nutrien Agar (NA) untuk Pertumbuhan Bakteri Stapylococcus aureus. Prosiding Seminar Kesehatan Perintis, 4(2), 74–79. Retrieved from https://jurnal.upertis.ac.id/index.php/PSKP/article/view/720
Jawetz, E., Melnick, J.L., Adelberg, E.A., Brooks, G.F., Butel, J.S., & Ornston, L.N. (2005). Mikrobiologi Kedokteran. Jakarta, Indonesia: EGC.
Kader, A.J., & Omar, O. (1998). Isolation of Cellulolytic Fungi from Sayap-Kinabalu Park, Sabah. Serawak. ASEAN Review of Biodiversity and Environmental Conservation (ARBEC), 1–6.
Lestari, D.P.Y. (2016). Pertumbuhan Bakteri Bacillus subtilis pada Media Biji Nangka dan Biji Kluwih sebagai Substitusi Media NA (Nutrient Agar) [Naskah Publikasi]. Surakarta, Indonesia: Universitas Muhammadiyah Surakarta. Retrieved from https://eprints.ums.ac.id/42894/1/NASKAH%20PUBLIKASI.pdf
Marheni, M., Martono, E., & Sijabat, O.S. (2021). Exploration of Symbiotic Bacteria of Oryctes rhinoceros (Coleoptera: Scarabaeidae) Larvae from Oil Palm Empty Fruit Bunches. AGRIVITA Journal of Agricultural Science, 43(1), 190–197. https://doi.org/10.17503/agrivita.v43i1.2301
Mulyasari, M., Melati, I., & Sunarno, M.T.D. (2015). Isolasi, Seleksi, dan Identifikasi Bakteri Selulolitik dari Rumput Laut Turbinaria sp. dan Sargassum sp. sebagai Kandidat Pendegradasi Serat Kasar Pakan Ikan [Isolation, Selection and Identification of Cellulolytic Bacteria from Seaweed as a Candidate of Crude Fibre Degradation]. Jurnal Riset Akuakultur, 10(1), 51–60. http://doi.org/10.15578/jra.10.1.2015.51-60
Nafion, N., Putri, D.H., & Irdawati, I. (2019). Optimization of Medium Fermentation for Production of Antimicrobial Compounds by Endofit Bacteria Andalas Plant (Morus macroura Miq.) B.J.T.A-6 Isolate. Bioscience, 3(1), 79–84. https://doi.org/10.24036/0201931102865-0-00
Pandanwangi, S.T.W., Nabila, E.R., & Nurpatmawati, N. (2023). Pembuatan Media Pertumbuhan Bakteri Menggunakan Tepung Biji Nangka (Artocarpus heterophyllus Lamk) untuk Pertumbuhan Bakteri Staphylococcus aureus [Preparation of Bacterial Growth Media Using Jackfruit Seed Flour (Artocarpus heterophyllus Lamk) for Staphylococcus aureus Bacterial Growth]. Praeparandi: Jurnal Farmasi dan Sains, 7(1), 1–11.
Parnanto, N.H.R., Setyowati, R., & Utami, R. (2013). Kapasitas Antioksidan dan Kemampuan Antimikrobia pada Daun Kirinyuh (Eupatorium odoratum) selama Penyimpanan Ikan Tongkol (Euthynnus affinis) pada Suhu Dingin [Antioxidant Capacity and Antimicroorganism Ability of Kirinyuh Leaves (Eupatorium odoratum) of Tuna Fish (Euthynnus affinis) during Cold Storage]. Jurnal Teknologi Hasil Pertanian, 6(1), 36–45. https://doi.org/10.20961/jthp.v0i0.13504
Paul, S.I., Rahman, M.M., Salam, M.A., Khan, M.A. R., & Islam, M.T. (2021). Identification of Marine Sponge-Associated Bacteria of the Saint Martin’s Island of the Bay of Bengal Emphasizing on the Prevention of Motile Aeromonas septicemia in Labeo Rohita’, Aquaculture, 545, 737156. https://doi.org/10.1016/J.AQUACULTURE.2021.737156
Radji, M. (2009). Buku Ajar Mikrobiologi: Panduan Mahasiswa Farmasi & Kedokteran. Jakarta, Indonesia: EGC.
Rahman, R., Widarti, W., Kalma, K., & Nihad, N. (2020). Efektivitas Berbagai Konsentrasi Serbuk Kedelai (Glycine max (L.) Merill) sebagai Bahan Pengganti Pepton pada Media Pertumbuhan Candida albicans [Effectiveness of Various Concentrations of Soybean Powder (Glycine max (L.) Merill) as Peptone Substitute of Candida albicans Growth Medium]. Jurnal Media Analis Kesehatan, 11(1), 40–46. https://doi.org/10.32382/mak.v11i1.1554
Shelomi, M., & Chen, M. (2020). Culturing-Enriched Metabarcoding Analysis of the Oryctes rhinoceros Gut Microbiome. Insects, 11(11), 782. https://doi.org/10.3390/insects11110782
Sitanggang, F.S.D. (2021). Respon Pertumbuhan Bibit Kelapa Sawit dengan Menggunakan TKKS Terdekomposisi Bakteri Simbion Larva Oryctes rhinoceros (Coleoptera: Scarabidae) dan Pupuk NPK [Doctoral dissertation]. Medan, Indonesia: Universitas Sumatera Utara.
Wartono, W., & Priatno, T.P. (2009) Pertumbuhan Bakteri Photorhabdus luminescens pada Berbagai Media dan Produksi Eksotoksin sebagai Racun Serangga [Growth of Photorhabdus luminescens on Several Culture Media and its Exotoxic Production as Insecticide]. Jurnal Entomologi Indonesia, 6(2), 60–69. https://doi.org/10.5994/jei.6.2.60
Yustinah, Y., Gozan, M., & Hermansyah, H. (2016). Pengaruh Jenis Sumber Nitrogen pada Pembuatan Olyhydroxybutyrate dari Glukosa Menggunakan Bakteri Bacillus cereus. Prosiding Semnastek, 2016, TK-017. Retrieved from https://jurnal.umj.ac.id/index.php/semnastek/article/view/722
Zamilah, M., Ruhimat, U., & Setiawan, D. (2020). Media Alternatif Kacang Tanah untuk Pertumbuhan Bakteri. Journal of Indonesian Medical Laboratory and Science (JoIMedLabS), 1(1), 57-65. https://doi.org/10.53699/joimedlabs.v1i1.11
DOI: https://doi.org/10.22146/jpti.99959
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