Dekontaminasi Mycobacterium avium subspecies paratuberculosis pada feses menggunakan beberapa jenis desinfektan

Ika Suharti(1*), Ni Luh Putu Ika Mayasari(2), Fachriyan Hasmi Pasaribu(3)

(1) Balai Uji Terap teknik dan Metode Karantina Pertanian
(2) Fakultas Kedokteran Hewann, Institut Pertanian Bogor
(3) Fakultas Kedokteran Hewann, Institut Pertanian Bogor
(*) Corresponding Author


Paratuberculosis or Johne’s Disease is a granulomatous enteritis chronic disease of domestic and wild ruminants caused by infection of Mycobacterium avium subspecies paratuberculosis. The disease commonly infects dairy cattle with clinical signs of chronic diarrhea, decreasing body weight, low milk production, oedema, anemia and occasionally infertility. The basic procedure in order to control Paratuberculosis in farms is to do a good and proper handling of animal faecal. Disinfection of animal environments such as pens, faecal, sewerage and sewage are important in prevention of transmission of this disease. The purpose of this research is to determine specific disinfectan and dosage for Mycobacterium avium subspecies paratuberculosis decontamination in cattle feces so  it can be applied as disease control measures. Cow's feces were contaminated with MAP 105CFU/ml and treated with ammonium quartener, phenolic and formaldehyde disinfectant doses 10%, 15% and 20%. The effectiveness of the disinfectant was tested based on MAP identification using Löwenstein-Jensen culture medium and nested Polymere Chain Reaction(PCR). The results showed 15% and 20% doses of formaldehyde disinfectants efective to decontaminate Mycobacterium avium subspecies paratuberculosis  in catle feces.


Disinfectant; Feces; Mycobacterium avium subspecies paratuberculosis

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Adj, R. S. 2004. Isolasi dan uji serologi terhadap Mycobacterium paratuberculosis pada sapi perah. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. Pusat Penelitian dan Pengembangan Peternakan, Badan Penelitian dan Pengembangan Pertanian.p281-284. / fullteks semnas/pro04-44.pdf. Diakses pada 4 maret 2014.

Adji, R.S. (2008). Deteksi Mycobacterium avium subspesies paratuberculosis pada sapi perah di Kabupaten Bandung dan Banyumas. Tesis. Sekolah Pascasarjana, Institut Pertanian Bogor, Bogor.

Blackwell, M. 2004. Farm planning ready to beat emergency disease outbreaks, poultry world. September 2004. Proquest Agriculture Journal. 24. Diakses pada 20 april 2014.

Bowman, G.L. and W.P. Shulaw. 2008. Disinfection in On-Farm Biosecurity Procedures. Ohio State University Extension Fact Sheet. Veterinary Preventive Medicine. VME-0008-01:http;// Diakses pada 20 April 2014.

Butcher, W. and D. Ulaeto. (2010). Contact of activation of Orthopoxviruses By Houshold Disinfectans. Departemen of Biomedical Sciences, Dstl Porton Down. Philadhelphia. 279-283.

Collins, M.T. and E. Manning. (2004). Paratuberculosis in Dairy Cattle. School of Veterinary Medicine. Madison, University of Winconsin. Wincosin.

[EAZWV] Europe Association of Zoo and WildLife Veterinary (BE). 2008. Paratuberculosis or Johne’s Diseases. EAZWV Transmissible Diseases Fact Sheet. Sheet No.91. 20Johne%20s%20Disease.doc.pdf. Diakses pada 15 Mei 2014.

Gaman, P.M. and K.B. Sherington. (1992). Pengantar Ilmu Pangan Nutrisi dan mikrobiologi. Gajah Mada University Press. Yogyakarta.

Greig, A., K. Stevenso, D. Henderson, V. Perez, V. Hughes, I. Pavlik, M. Hinesil, I. McKendrick, and J.M. Sharp. (1999). Epidemiological study of paratuberculosis in wild rabbits in Scotland. J Clin Microbiol. 37(6):1746–1751.

Hasanova, L. And I. Pavlik. (2006). Economic impact of partuberculosis in dairy cattle herd: areview. Vet Med. 51(5):193-211

Jay, J.M. (2000). Modern Food Microbiology. Aspen Publicher, Inc Gheitrburg, Maryland.

Kalis, C.H.J., J.W. Hesselink, H.W. Barkema, and M.T.Collins. (2000). Culture of strategically pooled bovine fecal samples as a method to screen herds for paratuberculosis. J Vet Diagn Invest. 12:547-551.

Lawley, R., L. Curtis, and J.Davis. (2008). The Book Safety Hazard Gudebook. Cambrige (UK): The Royals society of Chemistry.

Nugroho, W.S. (2008). Deteksi Mycobacterium avium subspecies paratuberculosis pada sapi perah, susu pasteurisasi, dan susu formula lanjutan di wilayah Bogor. Disertasi. Sekolah Pascasarjana, Institut Pertanian Bogor, Bogor.

[OIE] Office Internationale des Epizooties (FR). (2012). Paratuberculosis. In : Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. World Organization for Animal Health. Paris.347–349

Rismana E. 2002. Mengenal bahan kimia desinfeksi. /07/cakrawala/index.htm. Diakses pada 4 April 2014.

Rutala, W.A. and D.J.Weber. (1999). Disinfection of endoscopes: review of new chemical sterilants used for high-level disinfection. Infect Control Hosp Epidemiol. 20(1):69–7

Setyowati, R., E. Mudigdo, M.A. Gani Susanto, Trimurti and A.Azmi. (1986). Pengkajian

paratuberkulosis (Johne’s disease) pada sapi dan kerbau. Bulletin Veteriner.4:1–4.

Shin, S.J., Y.F.Chang and C. Huang. (2004). Development of Polymerase Chain Reaction Test to Confirm Mycobacterium avium subspecies paratuberculosis in Culture. J Vet Diagn Invest. 16(2):116-120.

Siswandoko dan B. Soekarjo. (1995). Kimia Medisinal. Airlangga University Press, Surabaya.

Somani, S.B., W.N. Ingole and S.N.Kulkarni. (2011). Disinfection of Water by Using Sodium Chloride (NaCl) and Sodium Hypochlorite (NaOCl). J Eng Reseach Stud. 2(4):40-43

Stewart, C.M. (2003). Staphylococus aureus and Staphylococcal enterotoxin. Ch.12 In: Hocking AD (Eds).Foodborne Microorganism Of Publict Health Significance.Australian Institute of Food Science and Technology. Sidney: 359-280

[USDA] United State Departement of Agriculture (US). (2001). Foodborne Pathogenic Microorganism and Natural Toxin Handbook. Center food Safety & Aplied Nutriton. Washington.

Wu, C.W., M. Livesey, S.K. Schmoller, E.J.B. Manning, H. Steinberg, W.C.Davis, M.J. Hamilton and A.M. Talaati. (2007). Invasion and Persistence of Mycobacterium avium subsp. paratuberculosis during Early Stages of Johne’s Disease in Calves. J Infect Immun. 75:2110–211.

Vansnick, E., D.P. Rijk, F. Vercammen, D. Geysen, L. Rigouts and F. Portaels. (2004). Newly Developed Primers for The Detection of Mycobacterium avium subspecies paratuberculosis. Vet Microbiol. 100:197–204.


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