* Corresponding Author
Efektivitas Ekstrak Biji Kapas (Gossypium hirsutum L.) terhadap Jumlah dan Viabilitas Embrio Mencit (Mus musculus L.)
https://doi.org/10.22146/jsv.27563 Nofri Zayani(1*), Iman Supriatna(2), Mohamad Agus Setiadi(3)
(1) Fakultas Kedokteran Hewan, Institut Pertanian Bogor
(2) Fakultas Kedokteran Hewan, Institut Pertanian Bogor
(3) Fakultas Kedokteran Hewan, Institut Pertanian Bogor
(*) Corresponding Author
Abstract
The cottonseed (Gossypium hirsutum L.) contained gossypol as antifertility agent. The effect of cottonseed extract treatment could be decrease and impaired follicles development were accompanied by oocytes
damage. Damage of oocytes resulted reduction of number and viability of embryos. The objective of this study was to evaluate the effectiveness of cottonseed extract (Gossypium hirsutum L.) on the number and viability of
mice embryo (Mus musculus L.). Doses of the cottonseed extract were used consists of 0 (control), 1.5; 2.1; and 2.7 g/kg of body weight (BW) for 24 days via the oral route. This research used 24 animals healthy of female
DDY mice 14-15 weeks old and 30-35 g BW. Embryos were collected at day 4 of pregnancy by flushing the utery cornua. The collected embryos were cultured in vitro for 48 hours in modified phosphate buffered saline (PBS) supplemented with 10% fetal bovine serum (FBS) culture medium according to the stage of its development to observe the viability of embryos. The result showed that the cottonseed extract with doses 1.5; 2.1; and 2.7 g/kg of body weight (BW) made the number of embryos which collected in D4 of pregnancy significantly lower
than control (P<0.05). Data from embryos culture in vitro for 48 hours decreased embryos number (P<0.05) that developed in to the expanded and hatched blastocysts. At 2.7 g/kg BW, embryos only can develop to the
blastocysts stage. Retardation (4-8 cells) and degeneration embryos did not develop in culture
Keywords
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AVMA [American Veterinary Medical Association].(2013). Guidelines for the Euthanasia of Animals. Penerbit Meacham Road Pr.
Schaumburg: 38-39.
Bai, J. and Shi Y. (2002). Inhibition of T-type Ca2+
currents in mouse spermatogenic cells by
gossypol, an antifertility compound. Eur. J.
Pharmacology. 440:1-6.
Basini, G., Bussolati, S., Baioni, L. and Grasselli, F.
(2009). Gossypol, a polyphenolic aldehyde from
cotton plant interferes with swine granulosa cell
function. Dom. Anim. Endocrinol.37:30-36.
Brocas, C., Rivera, M. R., Paula-Lopes, F. F., McDowell,
L. R., Calhoun, M. C., Staples, C. R., Wilkinson,
N. S., Boning, A. J., Chenoweth, P. J. and Hansen,
P. J. (1997). Deleterious actions of gossypol on
bovine spermatozoa, oocytes, and embryos. Biol.
Reprod. 57:901-907.
Camara, A. C. L., Gadelha, I. C. N., Borges, P. A. C.,
Paiva, S. A., Melo, M. M. and Blanco, B. S.
(2015). Toxicity of gossypol from cottonseed
cake to sheep ovarian follicles. Research
Article Plos One. 10:1-11.
Cecconi, S. (2002). Growth and differentiation of small
ovarian follicles in mammals: problems and
future perspective. Reprod. Dev. 48: 431-445.
Cheng, J. S., Lo, K. Y., Yeh, J. H., Cheng, H. H., Liu,
C. P., Chen, W. C. and Jan, C. R. (2003). Effect
of gossypol on intracellelar Ca2+ regulation in
human hepatoma cells. Chin. J. Physiol. 46:
-122.
Gadelha, I. C. N., Fonsesa, N. B. S., Oloris, S. C.
S., Melo, M. M. and Blanco, S. B. (2014a).
Gossypol toxicity from cottonseed products.
Sci. World. 14:1-9.
Gadelha, I. C. N., deMacedo, M. F., Melo, M. M.and
Blanco, B. B. (2014b). Gossypol promotes
degeneration of ovarian follicles in rats. Sci.
Word. 14: 1-7.
Galvao, K. N., Santos, J. E. P., Coscioni, A. C.,
Juchem, S. O., Chebel, R. C., Sischo, W. M.
and Villasenor, M. (2006). Embryo survival
from gossypol feed heifers after transfer to
lactating cows treated with human chorionic
gonadotropin. Dairy. Sci. 23:123-131.
Gordon, I. (2003). Laboratory Production of Cattle
Embryos 2rd ed. Penerbit CABI Publishing.
Willingford. 225-226.
Gu, Y., Lin, Y. C. and Rikihisa, Y. (1991). Inhibitory
effect of gossypol on steriodogenic pathways
in cultured bovine luteal cells. Biochem.
Biophysic. Res. Com. 61:169-179.
Hernandez, E. (2016). Cottonseed. Article Food. Sci.
:1-5.
Herve, J. C., Bastide, F. P. B., Cronier, L., Verrecchia,
F., Malassine, A. and Joffre, M. (1996).
Contraceptive gossypol blocks cell to cell
communication in human and rat cells. Eur. J
Pharm. 313: 242-255.
Kovacic, P. (2003). Mechanism of drug and toxic
actions of gossypol: focus on reactive oxygen
species and electron transfer. J. Curr. Medic.
Chem. 10: 2711-2718.
Kurniawati, H. (2010). Hubungan pemakaian
kontrasepsi pil KB kombinasi dengan tekanan
darah tinggi pada wanita pasangan usia subur
di Puskesmas Kecamatan Grogol Petamburan
Kota Administrasi Jakarta Barat tahun 2010.
Tesis. Fakultas Kesehatan Masyarakat,
Universitas Indonesia, Depok.
Lin, Y. C., Gu, Y., Brueggemeier, R. W. and Rikihisa,
Y. (1992). Binding of 3H-Gossypol in
organelles of cultured bovine luteal cells. Life.
Sci. 50:443-447.
Lin, Y. C., Sanbuissho, A., Coskun and Rikihisa, Y.
(1994). Inhibition of in vitro fertilization and
early embryonic development in hamsters by
gossypol. Life Sci. 55:14-19.
Lonergan, P., Rizos, D., Ward, F. and Boland, M. P.
(2001). Factors influencing oocyte and embryo
quality in cattle. Reprod. Nutr. Dev. 41: 427-437.
Nawrot, T. S., Hond, E. D., Fagard, R. H.,
Hoppenbrouwers, K. and Staessen, J. A. (2003).
Blood pressure, serum total cholesterol, and
contraceptive pill use in 17 year old girls. Eur
Cardiovasc Pretention Rehab. 10:438-442.
Nasim, A., Schrick, F. N., Butcher, R. L. and Inskeep,
E. K. (1995). Effect of persistent follicles
on early embryonic losses in beef cow. Biol
Reprod. 52:1129-1135.
Neganova, I. E. G. G., Sekirina and Ritter, U. E.
(2000). Surface-expressed E-cadherin, and
mitochondrial and microtubule distribution in
rescue of mouse embryos from 2-cell block by
aggregation. Mol. Hum. Reprod. 6: 454-46.
Pan, L. C., Nadakavukaren, M. J. and Jensen, D.
R. (1987). Effect of ingested gossypol on the
ultrastucture of rat ovarian follicles. Cell. Tiss.
Res. 250:215-220.
Ramadhani, S. A. (2016). Pengendalian folikulogenesis
ovarium mencit (Mus musculus) dengan pemberian
ekstrak biji kapas (Gossypium hirsutum).
Tesis. Institut Pertanian Bogor. Bogor: 24.
Rusmiati. (2010). Pengaruh ekstrak methanol kulit
kayu durian (Durio zibethinus Murr) pada
struktur mikroanatomi ovarium dan uterus
mencit (Mus musculus L.) betina. Sains Terap
Kim. 4: 29-37.
Sahid, M.dan Wahyuni, S. A. (2001). Kapas: Keragaan
dan Konsep Perbaikan Pengembangan Kapas
Di Indonesia. Penerbit Balittas. Malang: 1-16.
Said, S., Astirin, O. P. dan Wahyuningsih, S. (2011).
Tingkat fertilisasi dan perkembangan embrio
mencit yang diberi ekstrak buah merah. Med
Petern. 34: 112-116.
Sirard, M. A., Richard, F. O., Blondin, P. And Robert,
C. (2006). Contribution of the oocyte to
embryo quality. Theriogenology. 65:126-136.
Supriatna, I., Tolihere, M. R., Yusuf , T. L. dan
Purwantara, B. (1993). Pengaruh penambahan
fetal serum, calf serum, dan bovine serum
dalam pupukan in vitro terhadap viabilitas
embrio mencit. Laporan Penelitian. Institut
Pertanian Bogor. Bogor: 23
Theiler, K. (1989). The House Mouse:Atlas of
Embryonic Development. Penerbit Springer
Verlag Inc. Switzerland: 2-12.
Velasquiez-Pereira, J., Risco, C. A., McDowell, L.
R., Staples, C. R., Prichard, D., Chenoweth, P.
J., Martin, F. G., Williams, S. N., Rojas, L. X.,
Calhoun, M. C. and Wilkinson, N. S. (2002).
Long term effect of feeding gossypol and
vitamin E to dairy calves. Dairy Sci.82:1240-1251.
Villasenor, M., Coscioni, A. C., Galvao, K. N.,
Chebel, R. C. and Santos, J. E. P. (2008).
Gossypol distrupt embryos development in
Heifers. Dairy. Sci. 91: 3015-3024.
Yuan, Y. Y. and Shi, Q. X. (2000). Inhibition of
hamster sperm acrosomal enzyme by gossypol
is closely associated with the decrease in
fertilization capacity. Contraception. 62: 203-209.
DOI: https://doi.org/10.22146/jsv.27563
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