Identifikasi resistensi ragam antibiotik terhadap Escherichia coli yang diisolasi dari karkas sapi Rumah Potong Hewan di Banyuwangi

https://doi.org/10.22146/jsv.30740

Faisal Fikri(1), Amung Logam Saputro(2), Iwan Sahrial Hamid(3), Muhammad Thohawi Elziyad Purnama(4*)

(1) Departemen Kedokteran Dasar Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
(2) Departemen Klinik Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
(3) Departemen Kedokteran Dasar Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
(4) Departemen Anatomi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
(*) Corresponding Author

Abstract


Tujuan penelitian ini adalah mengidentifikasi resistensi antibiotik dari Escherichia coli pada karkas sapi yang diambil dari rumah potong hewan di Kabupaten Banyuwangi. Sampel dikoleksi dari Kecamatan Banyuwangi, Rogojampi, Genteng dan Kalibaru. Penelitian ini menggunakan kajian cross sectional dengan prevalensi diasumsikan 50% setiap cemaran bakteri, tingkat kepercayaan 95% dan tingkat kesalahan 10%, sehingga didapatkan 96 sampel yang harus diambil untuk diuji. Hasil penelitian menunjukkan tujuh sampel (7.3%) positif E. coli. Hasil resistensi E. coli terhadap antibiotik golongan sefalotin (42,9%), trimethoprim (14,3%) dan eritromisin (42,9%) sedangkan ampisilin, enrofloksasin, kloramfenikol dan tetrasiklin masih dinyatakan sensitive.

Keywords


resistensi antibiotik, Banyuwangi, karkas sapi, Escherichia coli



References

Ahmad, I., Anjum, M. S., & Hanif, M. (2012). Prevalence of poultry diseases at high altitudes of district Poonch Azad Jammu & Kashmir. Pakistan Journal of Science, 64(4).

Anacona, J., Pineda, Y., Bravo, A., & Camus, J. (2016). Synthesis, characterization and antibacterial activity of a tridentate Schiff base derived from cephalexin and 1, 6-hexanediamine and its transition metal complexes. Med Chem, 6, 467-473.

Anil, M. H., Love, S., Helps, C. R., & Harbour, D. A. (2002). Potential for carcass contamination with brain tissue following stunning and slaughter in cattle and sheep. Food Control, 13(6-7), 431-436.

Badan Standardisasi Nasional. 2008. SNI No. 2897:2008 Tentang Pengujian Cemaran Mikrobia dalam Daging, Telur, dan Susu serta Hasil Olahannya. Badan Standardisasi Nasional, Jakarta.

Bai, J., Paddock, Z. D., Shi, X., Li, S., An, B., & Nagaraja, T. G. (2012). Applicability of a multiplex PCR to detect the seven major Shiga toxin–producing Escherichia coli based on genes that code for serogroup-specific O-antigens and major virulence factors in cattle feces. Foodborne pathogens and disease, 9(6), 541-548.

Chaudhuri, R. R., & Henderson, I. R. (2012). The evolution of the Escherichia coli phylogeny. Infection, Genetics and Evolution, 12(2), 214-226.

Chen, K. G., & Sikic, B. I. (2012). Molecular pathways: regulation and therapeutic implications of multidrug resistance. Clinical cancer research, 18(7), 1863-1869.

Cheng, Q., & Sun, D. W. (2008). Factors affecting the water holding capacity of red meat products: a review of recent research advances. Critical reviews in food science and nutrition, 48(2), 137-159.

Cheng, D., Zhu, S., Su, Z., Zuo, W., & Lu, H. (2012). Prevalence and isoforms of the pathogenicity island ETT2 among Escherichia coli isolates from colibacillosis in pigs and mastitis in cows. Current microbiology, 64(1), 43-49.

Clinical and Laboratory Standards Institute. 2012. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplement. Clinical and Laboratory Standards Institute, West Valley.

Erfianto GI. 2014. Escherichia coli yang Resisten terhadap Antibiotik yang Diisolasi dari Sapi Potong yang Diimpor melalui Pelabuhan Tanjung Priok Jakarta.

Fikri, F., Hamid, I. S., & Purnama, M. T. E. (2017). Uji organoleptis, pH, uji eber dan cemaran bakteri pada karkas yang diisolasi dari kios di Banyuwangi. Jurnal Medik Veteriner, 1(1), 23-27.

Fraser, T. N., Avellaneda, A. A., Graviss, E. A., & Musher, D. M. (2012). Acute kidney injury associated with trimethoprim/sulfamethoxazole. Journal of antimicrobial chemotherapy, 67(5), 1271-1277.

Gerberding, J. L., Snider, D. E., Popovic, T., Thacker, S. B. 2004. Diagnosis and management of food borne illnesses. Morbidity and Mortality Weekly Report (MMWR) 53:4.

Guilfoile, P., & Alcamo, I. E. (2007). Antibiotic-resistant bacteria. Infobase Publishing.

Haileselassie, M., Taddele, H., Adhana, K., & Kalayou, S. (2013). Food safety knowledge and practices of abattoir and butchery shops and the microbial profile of meat in Mekelle City, Ethiopia. Asian Pacific journal of tropical biomedicine, 3(5), 407-412.

Hossain, M. K., Rahman, M., Nahar, A., Khair, A., & Alam, M. M. (2013). Isolation and identification of diarrheagenic Escherichia coli causing colibacillosis in calf in selective areas of Bangladesh. Bangladesh Journal of Veterinary Medicine, 11(2), 145-149.

Hur, J., Jawale, C., & Lee, J. H. (2012). Antimicrobial resistance of Salmonella isolated from food animals: A review. Food Research International, 45(2), 819-830.

Kartikasari, A. M., Hamid, I. S., Purnama, M. T. E., Damayanti, R., Fikri, F., & Praja, R. N. (2019). Isolasi dan Identifikasi Bakteri Escherichia coli Kontaminan Pada Daging Ayam Broiler Di Rumah Potong Ayam Kabupaten Lamongan. Jurnal Medik Veteriner, 2(1), 66-71.

Kusumaningsih, A. (2010). Beberapa Bakteri Patogenik Penyebab Foodborne Disease. Wartazoa, 20(3), 103.

Mandala, A. Y., Swacita, I. B. N., & Suada, I. K. (2016). Penilaian penerapan animal welfare pada proses pemotongan sapi di rumah pemotongan hewan Mambal Kabupaten Badung. Indo Med Vet, 5(1), 1-12.

Pitout, J. D. (2012). Extraintestinal pathogenic Escherichia coli: an update on antimicrobial resistance, laboratory diagnosis and treatment. Expert review of anti-infective therapy, 10(10), 1165-1176.

Popowska, M., Rzeczycka, M., Miernik, A., Krawczyk-Balska, A., Walsh, F., & Duffy, B. (2012). Influence of soil use on prevalence of tetracycline, streptomycin, and erythromycin resistance and associated resistance genes. Antimicrobial agents and chemotherapy, 56(3), 1434-1443.

Rosano, G. L., & Ceccarelli, E. A. (2014). Recombinant protein expression in Escherichia coli: advances and challenges. Frontiers in microbiology, 5, 172.

Saidi, B., Mafirakureva, P., & Mbanga, J. (2012). Antimicrobial resistance of Escherichia coli isolated from chickens with colibacillosis in and around Harare, Zimbabwe. Avian diseases, 57(1), 152-154.

Sanchez, G. V., Master, R. N., Karlowsky, J. A., & Bordon, J. M. (2012). In vitro antimicrobial resistance of urinary Escherichia coli isolates among US outpatients from 2000 to 2010. Antimicrobial agents and chemotherapy, 56(4), 2181-2183.

Smith, R., & Coast, J. (2013). The true cost of antimicrobial resistance. Bmj, 346, f1493.

Sundara I. 2015. Angka lempeng total dan cemaran Escherichia coli pada peralatan pemotongan di tingkat pedagang ayam tradisional Kota Pekanbaru. [Doctoral dissertation] Universitas Islam Negeri Sultan Syarif Kasim Riau.

Tao, F., Peng, Y., Li, Y., Chao, K., & Dhakal, S. (2012). Simultaneous determination of tenderness and Escherichia coli contamination of pork using hyperspectral scattering technique. Meat Science, 90(3), 851-857.



DOI: https://doi.org/10.22146/jsv.30740

Article Metrics

Abstract views : 421

Refbacks

  • There are currently no refbacks.




Copyright (c) 2023 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

    CrossrefROADCOREProduct DetailsDESKRIPSI GAMBAR


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

HP. 0895363078367

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats