Klebsiella pneumoniae Isolated from Subclinical Mastitis Milk of Etawah Crossbreed Goat


Clara Ajeng Artdita(1*), Fajar Budi Lestari(2), Achmad Fauzi(3), Erian Pemila Ayu Tanzila(4)

(1) Vocational College Universitas Gadjah Mada, Yogyakarta
(2) Vocational College Universitas Gadjah Mada, Yogyakarta
(3) Vocational College Universitas Gadjah Mada, Yogyakarta
(4) Vocational College Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author


Klebsiella spp. is a common bacteria causing mastitis. Klebsiella pneumoniae and Klebsiella varicola is the most infected Klebsiella spp in the dairy farm. This study used 16 milk samples from 8 lactation goats in Sahabat Ternak farm, Sleman, Yogyakarta. Samples were tested by california mastitis test (CMT) reagent to determine the mastitis status, inoculated on blood agar (BA) then on Mac Conkey Agar (MCA) as a selective media for Gram negative bacteria. The colonies from BA and MCA were stained by Gram staining to determine Gram-positive or Gram-negative bacteria and its cell morphology. The colonies from MCA were identified by biochemical tests such as sugar fermentation tests (glucose, lactose, maltose, and saccharose), arginine, ornithine decarboxylase, indole, citrate, urea hydrolysis (Christensen’s method), lysin and malonate. The result of mastitis test showed 5 goats were positive result and 3 samples were negative. Bacterial staining showed 2 samples were Gram-negative, rods and the others were Gram-positive, coccus. The samples with rods shapes were continued by biochemical tests. The characterization result of biochemical test indicated that the rods shapes bacteria were Klebsiella pneumoniae. These bacteria identification conducted in Laboratorium Preklinis Program Studi Kesehatan Hewan, Sekolah Vokasi Universitas Gadjah Mada and the result confirmation using positive control of  Klebsiella pneumoniae conducted in Balai Laboratorium Kesehatan Yogyakarta. These results showed that milk from Etawah crossbreed goat was infected by Klebsiella pneumoniae.


Ettawah Crossbreed Goat; Subclinical Mastitis; Klebsiella pneumoniae

Full Text:



Ajuwape, ATP., Roberts, AA., Solarin, OO., and Adetosoye, AI. (2005). Bacteriological and haematological studies of Clinical Mastitis in Goats in Ibadan, OYO State, Nigeria. Small Ruminant Research. 60(3):307-310.

Andrews, WH. and Hammack, TS. (2001). Bacteriological Analytical Manual. Center for Food Safety and Applied Nutrition. Chap: Food Sampling and Preparation of Sample Homogenate.

Bannerman, D.D., Paape, M.J., Hare, W.R., and Sohn EJ. (2003). Increased levels of LPS-binding protein in bovine blood and milk following bacterial lipopolysaccharide challenge. J. Dairy Sci. 86,:3128-3137.

Carter, GR. (1979). Diagnostic Procedures in Vterinary Bacteriology and Mycology. Third edition. Pp:82-93. USA, Charless C. Thomas Publisher.

Carter GR and Wise, DJ. (2004). Veterinary Bacteriology and Mycology. Sixth edition. pp:132-134.Iowa:,Iowa State Press.

Collin, CH., Lyne, PM., Grange, JM., and Falkinham, JO. (2004). Collin and Lyne’s Microbiological Methods. Eight edition. Pp: 61-81, 282-288. London, Arnold.

Christina, S., Pettersson-Wolfe, Isis, K., Mularky, and Currin, J. (2011). Klebsiella spp.: A Practical Summary for Controlling Mastitis. Article. Virginia Tech.

Forbes, BA., Shamn, DF., and Weisffeld, AS. (2002). Bailey and Scott’s Diagnostic Microbiology. Twelveth edition. Pp: 222-224, 226, 232-246. USA, Mosby.

Grohn YT, Wilson DJ, Gonzalez RN, Hertl JA, Schulte H, Bennet, G., and Schukken, YH. (2004). Effect of pathogen-specific clinical mastitis on milk yield in dairy cows. J Dairy Sci. 87: 3358–3374.

Guha, A., Guha, R., and Gera, S. (2012). Comparison of somatic cell count, California mastitis test, chloride test and rennet coagulation time with bacterial culture examination to detect subclinical mastitis in riverine buffalo (Babalus bubalis). African Jjournal of Agricultureal Research. 7(41):5578-5584.

Habrun B. and Kompes, G. (2014). Rarely isolated mastitis pathogens. Veterinarska Stanica. 45: 41–46.

Hall, SM, and Rycroft, AN. (2007). Causative organisms and somatic cell counts in subclinical intramammary infections in milking goats in the UK. Vet Rec.160 (1) : 19-22.

Jeph, NK., Chahar, A., Tanwar, RK., Fakhruddin and Mudgal, N. (2013). Clinico-therapoetic studies on Clinical Mastitis in Goats (Capra hircus). Veterinary Practitioner. 14 : 2.

MacFaddin J. (1985). Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williams and Wilkins, Baltimore.

McDougall, S., Pankey, W., Delaney, C., Barlow, J., Murdough, PA., and Scruton, D. (2002). Prevalence and incidence of subclinical mastitis in goats and dairy ewes in Vermont USA. Small Rumin Res. 46(2-3) : 115-121.

Munoz, MA., Ahlstrom, C., Rauch, B.., and Zadoks, RN. (2006). Fecal shedding of Klebsiella pneumoniaeby dairy cows. J. Dairy Sci. 89:3425-3430.

Munoz MA, Bennett GJ, Ahlström C, Griffiths, HM., Schukken, YH., and Zadoks, RN. (2008). Cleanliness scores as indicator of Klebsiella exposure in dairy cows. J Dairy Sci. 91(10):3908 –16.

Munoz, MA., Welcome, FL., Schukken, YH., and Zadoks, RN. (2007). Molecular epidemiology of two Klebsiella pneumoniaemastitis outbreaks on a dairy farm in New York State. J Clin Microbiol. 45: 3964–3971.

Ohnishi M, Okatani AT, Harada K, Sawada T, and Marumo K. (2013). Genetic Characteristics of CTX-M-Type Extended-Spectrum-b-Lactamase (ESBL)-Producing Enterobacteriaceae Involved in Mastitis Cases on Japanese Dairy Farms, 2007 to 2011. J Clin Microbiol. 51: 3117–3122.

Paape, MJ, and Capuco, AV. (1997). Cellular Defense Mechanisms in the Udder and Lactation of Goats. J Anim Sci. 75(2) : 556-565.

Persson Y, and Olofsson I. (2011). Direct and indirect measurement of somatic cell count as indicator of intramammary infection in dairy goats. Acta Vet Scand. 53(15) : 1-5.

Quinn, PJ., Carter, ME, Markey, B., and Carter, GR. (2004). Clinical Veterinary Microbiology. Pp: 209-221. USA, Mosby.

Radostits, O.M.; Gay, C.C.; Hincliff, K.W., and Constable, P.D. (Eds). (2009). Veterinary medicine: A textbook of the diseases of cattle, horses, sheep, pigs, and goats. Tenth edition. Philadelphia: Saunders. p.673-762.

Schukken YH, Hertl J, Bar D, Bennett GJ, Gonzalez RN, Rauch BJ, Santisteban C, Schulte HF, Tauer L, Welcome FL, and Gröhn YT. (2009). Effects of repeated gram-positive and gram-negative clinical mastitis episodes on milk yield loss in Holstein dairy cows. J Dairy Sci. 92: 3091–3105.

Sirois, M. (2015). Laboratory Procedures for Veterinary Technicians. Sixth edition. Pp:2587-261. Missouri, Elsevier Mosby.

Wooding, F.B.P., M. Peaker, and J. L. Linzell. (1970). Theories of milk secretion: Evidence from the electron microscopic examination of milk. Nature (Lond.) 226:762.

Zadoks, RN., Middleton, JR., McDougall, S., Katholm, J., and Schukken, YH. (2011). Molecular epidemiology of mastitis pathogens of dairy cattle and comparative relevance to humans. J Mammary Gland Biol Neoplasia. 16: 357–372.

DOI: https://doi.org/10.22146/jsv.34319

Article Metrics

Abstract views : 3683 | views : 2056


  • There are currently no refbacks.

Copyright (c) 2018 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

HP. 0895363078367

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


web stats View My Stats