Optimalisasi Pembekuan Sperma Limbah Kauda Epididimis Kambing Lokal dengan Metode Bertahap dan Stabilisasi

https://doi.org/10.22146/jsv.34663

Naela Wanda Yusria Dalimunthe(1*), M. Rosyid Ridlo(2), Agung Budiyanto(3)

(1) Fakultas KEdokteran Hewan Universitas Gadjah Mada
(2) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(3) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Buck slaugthering produce waste such as testicles including epididymis which contain fertile sperm. Utilization of cauda epididymis as the sources of sperm for producing goat frozen sperm was not reported yet. The aims of this study were improving the frozen-thawed sperm using stabilization and multistep methods which recovered from the waste of buck slaughtering as the source of sperma. Cauda epididymis spermatozoa which was washed then diluted using extender 1 (Tris-citrate-antibiotics) and extender 2 (extender 1- glycerol-egg yolk). The extender 2 addition was performed by single or multistep methods then freezed. Modification in the pre freezing proces were performed by comparing the conventional equilibration and stabilization methods. The sperm suspension was incubated in 4°C for 2 hours after filling-sealing into straws on the equilibration group whether the stabilization group was cooled in tube 15 mL. All cooled straws from both groups were placed 4 cm horizontally on liquid nitrogen surface for 10 minutes and then plunged into liquid nitrogen for storage. The evaluation of motility parameters such as pattern of the movement and motility percentation were done followed the standard methodology. The student t-test, correlation and one-way ANOVA were used for data analysis with P<0.05. The results showed that multistep dilution method could increase the motility (25.0 ± 1.8 %) compared with single step (18.3 ± 1.7 %). Pre freezing method with stabilization also resulted higher motility (24.2 ± 2.0 %) than equilibration method (17.5 ± 2.8 %). The pattern of the movement were not different between all methods and its combination. The multistep dilution method and stabilization cooling method as well as its combination seems could increase the quality of frozen-thawed cauda epididymis spermatpzoa of local buck.


Keywords


sperm, cauda epididymis; cryopreservation; stabilization; multistep

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References

Abba ,Y. and Igbokwe, I. O. (2015) Testicular and Related Size Evaluations in Nigerian Sahel Goats with Optimal Cauda Epididymal Sperm Reserve. Veterinary Medicine International. Article ID 357519. http://dx.doi.org/10.1155/2015/357519

Abu, A.H., Kisani, A.I., Ahemen, T. (2016) Evaluation of sperm recovered after slaughter from cauda epididymides of red Sokoto bucks. Veterinary World. 9(12):1440-1444.

Ahmad, M., Nasrullah, R., Ahmad, N., (2015) Effect of cooling rate and equilibration time on pre-freeze and post-thaw survival of buck sperm. Cryobiology. 70:233–238.

Cooper, T.G., and Yeung, C.H. (2006) Sperm maturation in the human epididymis. In The Sperm Cell. Production, Maturation, Fertilization, Regeneration. pp. 72–107, Cambridge University Press. Cambridge. UK.

Gautane, J.J., Balagan, E.J.Y., Ii, F.V. M., Ocampo, M.B., and Ocampo, L.C. (2016) Characteristics of epididymal sperm recovered from slaughterhouse derived testes of nondescript/native goats in the Philippines. Journal of Agricultural Technology. 12(2):215-228.

Hafez, E. S. E. 2000. Anatomy of Male Reproduction. In: Hafez E. S. E. & B. Hafez. Reproduction in Farm Animal. 7thed. Lippincott Williams & Wilkins. USA. Pp: 365-369

Joseph, H.Y., and Hinton, B.T., (2009) Development and morphogenesis of the Wolffian / epididymal duct, more twists and turns. Developmental Biology. 325(1):6–14.

Katanbafzadeh, H., Barati, F., Tabandeh, M. (2015) A new approach for cryoprotectant-free freezing of goat epididymal spermatozoa. Andrologia. 47:1093–1097.

Noviana, C., Boediono, A. dan Wresdiyati, T. (2000). Morfologi dan histomorfometri testis dan epididymis kambing kacang (capra sp.) Dan domba lokal (ovis sp.). Media Veteriner. 7(2): 12-16.

Olaciregui, M., Gil, L., Montón, A., Luño, V., Jerez, R.A., Martí, J.I. (2014) Cryopreservation of epididymal stallion sperm. Cryobiology. 68:91–95.

Pathak, A., Katiyar, R.S., Sharma, D.N., Farooqui, M.M. (2014) Postnatal Developmental Anatomy of Testes and Epididymis of Gaddi Goats. Int. J. Morphol. 32(4):1391-1398.

Perera, K.U.E., Ariyaratne, H.B.S. (2013) Cryopreservation of goat sperms collected from different regions of the epididymis. Int. J. Sci. Res. 2(7):36-38.

Rizal, M. (2006) Fertilitas Semen Beku Hasil Ejakulasi dan Spermatozoa Beku Asal Cauda Epididimis Domba Garut. Jurnal Sain Veteriner. 24(1).

Roof, D.J., Bowley, S., Price, L.L., Matsas, D.J. (2012) Comparison of two commercial extenders for cryopreservation of goat semen without sperm washing. Theriogenology. 77: 412–420.

Salmani, H., Towhidi, A., Zhandi, M., Bahreini, M., Sharafi, M. (2014) In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen. Cryobiology. 68:276–280.

Setyawan, E.E., Cooper, T.G., Widiasih, D.A., Junaidi, A., Yeung, C.H. (2009) Effects of cryoprotectant treatments on bovine sperm function and osmolyte content. Asian Journal of Andrology. 11: 571–581.

Setyawan, E.M.N., Kim, M.J., Oh, H.J., Kim, G.A., Jo, Y.K., Lee, S.H., Choi, Y.B., Lee, B.C. (2015). Maintaining canine sperm function and osmolyte content with multistep freezing protocol and different cryoprotective agents. Cryobiology. 71: 344–349.

Toelihere, M.R. 1993. Inseminasi Buatan pada Ternak. Penerbit Angkasa. Bandung.

Turri, F., Madeddu, M., Gliozzi, T.M., Gandini, G., and Pizzi, F. (2014) Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds. Animal. 8(3):440–447.

Ugwu, S.O.C. (2009) Relationship between scrotal circumference, in situ testicular measurements and sperm reserves in the West African Dwarf Goats. African Journal of Biotechnology 8(7):1354–1357.



DOI: https://doi.org/10.22146/jsv.34663

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