Variasi Morfologi dan Deteksi Leucocytozoon caulleryi dengan Metode PCR pada Ayam Ras di Wilayah Endemis Indonesia

Endang Suprihati; Wiwik Misaco Yuniarti

doi:10.22146/jsv.34666

Variasi Morfologi dan Deteksi Leucocytozoon caulleryi dengan Metode PCR pada Ayam Ras di Wilayah Endemis Indonesia

https://doi.org/10.22146/jsv.34666

Endang Suprihati^(1*), Wiwik Misaco Yuniarti⁽²⁾

(1) Fakultas Kedokteran Hewan Universitas Airlangga
(2) Fakultas Kedokteran Hewan Universitas Airlangga
(*) Corresponding Author

Abstract

The purpose of this study was to analyze the variation in morphology and detection Leucocytozoon caulleryi by PCR in mtDNA genes cty b that attacking bred chickens in endemic areas of Indonesia. This study was divided into three stages, data collection of Leucocytozoonosis cases in endemic areas of Indonesia; identification of parasites Leucocytozoon caulleryi morphologically through microscopic examination; and the identification of Leucocytozoon caulleryi cyt b genes that attack bred chickens in endemic areas by PCR. The results showed that there are variations in morphology of Leucocytozoon caulleryi that attacking chicken in endemic areas of Indonesia. Gamete morphometry of L. caulleryi had an average length and width 18.233 ± 4.672 dan 12.934 ± 3.349 µm. Nested PCR clearly showed positive reaction of Leucocytozoon infections by amplicons in 600 bp and 03 bp length.

Keywords

Leucocytozoon caulleryi; cyt b gene; domesticated chicken; Indonesia

Full Text:

PDF

References

Aiello, S.E.,(2008). Leucocytozoonosis. Merck Veterinary Manual, 8th Ed. National Publishing, Philadelphia, Pennsilvania. 256

Chasar, A., Loiseau, C., Valkiunas, G., Iezhova, T.,Smith, T.B.,and Sehgal, R.N.M., (2009). Prevalence and diversity patterns of avian blood parasites in degraded African rainforest habitats . Mol. Ecol. 18: 4121–4133.

Cosgrove, C.L.,Day, K.P., and Sheldon, B.C., (2006). Coamplification of Leucocytozoon by PCR diagnostic test for avian malaria : A cautionary note. J. Parasitol. 92: 1362-1365.

Darmono, T.W., Jamil, I., and Santosa, D.A., (2006). Pengembangan penanda molekuler untuk deteksi Phylophthori palmivora pada tanaman kakao. Menara Perkebunan.74: 87 – 96.

Hellgren, O.J.,Waldenstrom,J., and Bensch, S.,(2004).A new PCR assay for simultaneous studies of Leucocytozoon, Plasmodium, and Haemoproteus from avian blood.J. Parasitol. 90: 797- 802.

Isobe, T., and Suzuki, K., (1987). Detection of Serum Antibody to Leucocytozoon caulleryi in Naturally Infected Chickens by ELISA. J. of Vet. Med. Sci., 49: 165-167.

Kvist, L., (2000). Phylogeny and Phylogeography of European Parids. Departement of Biology, Oulu University.

Martinsen, E.S., Paperna, I., and Schall, J.J., (2006). Morphological versus molecular identificationof avian Haemosporidia: an exploration of three speciesconcepts. Parasitol., 133:279–288.

Morii, T.,andFukuda, M., (2005). Observations on First-Generation Schizogony of Leucocytozoon caulleryi in Chickens.J. of Euk. Microbiol., 39:281–287.

Omori, S.,Sato, Y., Hirakawa, S.,Isobe, T., Yukawa, M., and Murata, K.,(2008). Two extra chromosomal genomes of Leucocytozoon caulleryi:completenucleotide sequences of the mitochondrial genome and existence of the apicoplast genome.Parasitol. Res.,103:953–957.

Perkins, S.L., and Schall, J.J., (2002). A molecular phylogeny of malarial parasites recoverd form ccytochrome b gene sequences. J.Parasitol.,88: 972-978.

Purwanto, B., (2009). Kalau ayam kena Malaria, Majalah Trobos, Edisi Juli.

Sato, Y.,Hagahira, M., Yamaguchi, T., Yukawa, M., and Murata, K., (2007). Phylogenetic Comparison of Leucocytozoon spp from Wild Birds of Japan. J. of Vet.Med.Sci., 69:55-59.

DOI: https://doi.org/10.22146/jsv.34666