Studi Distribusi Glukosa Transporter 4 pada Otot Skelet Ayam Kedu Cemani

Teguh Budipitojo; Ariana -; Tri Wahyu Pangestiningsih; Hery Wijayanto; Dwi Liliek Kusindarta; Dewi Kania Musana

doi:10.22146/jsv.34698

Studi Distribusi Glukosa Transporter 4 pada Otot Skelet Ayam Kedu Cemani

https://doi.org/10.22146/jsv.34698

Teguh Budipitojo^(1*), Ariana -⁽²⁾, Tri Wahyu Pangestiningsih⁽³⁾, Hery Wijayanto⁽⁴⁾, Dwi Liliek Kusindarta⁽⁵⁾, Dewi Kania Musana⁽⁶⁾

(1) Departemen Anatomi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Jl. Fauna No. 2 Karangmalang, Yogyakarta
(2) Departemen Anatomi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Jl. Fauna No. 2 Karangmalang, Yogyakarta
(3) Departemen Anatomi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Jl. Fauna No. 2 Karangmalang, Yogyakarta
(4) Departemen Anatomi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Jl. Fauna No. 2 Karangmalang, Yogyakarta
(5) Departemen Anatomi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Jl. Fauna No. 2 Karangmalang, Yogyakarta
(6) Departemen Anatomi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Jl. Fauna No. 2 Karangmalang, Yogyakarta
(*) Corresponding Author

Abstract

Glucose transporter (GLUT 4) is glucose transporter protein regulated by insulin, found in adipose tissue and striated muscle (skeletal and cardiac muscle). Kedu cemani chicken is one of Indonesia endemic animal, found in Kedu, Temanggung regency, Central Java. This study was required to complete microscopic documentation of Indonesia’s native biodiversity. The objective of this study was to clarify GLUT 4 distribution in skeletal muscle fibers of kedu cemani chicken by using avidin-biotin-peroxidase complex (ABC) immunohistochemistry method. This study was conducted by using pectorales major, biceps brachii, and biceps femoris muscle tissue from 5 kedu cemani chicken. The result showed that GLUT 4 immunoreactivity were detected in sarcolemma and myofibrils component of pectorales major, biceps brachii, and biceps femoris muscle tissue. Intensity of GLUT 4 immunorectivites increased from weak intensity in pectorales major muscle tissue, moderate intensity in biceps brachii muscle tissue, then strong intensity in biceps femoris muscle tissue. This result might motivate to further exploration about the other kedu cemani chicken specific features to complete microscopic documentation of Indonesia’s native biodiversity.

Keywords

kedu chicken; skeletal muscle; GLUT 4; immunohistochemistry; ayam kedu; otot skelet; immunohistokimia

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References

Bell, G.I., Murray, J.C., Nakamura, Y., Kayano, T., Eddy, R.L., Fan, Y.S., Byers, M.G., Shows, T.B. (1989) Polymorphic human insulin-responsive glucose-transporter gene on chromosome 17p13. Diabetes 38 (8): 1072–1075.

Birnbaum, M.J. (1989) Identification of a novel gene encoding an insulin-responsive glucose transporter protein. Cell 57 (2): 305–15.

Cushman, S.W, Wardzala, L.J. (1980) Potential mechanism of insulin action on glucose transport in the isolated rat adipose cell. Apparent translocation of intracellular transport systems to the plasma membrane. J. Biol. Chem. 255 (10): 4758–62.

Huang, C.C., Lee, C.C., Hsu, K.S. (2010) The role of insulin receptor signaling in synaptic plasticity and cognitive function. Chang Gung Med. J. 33 (2): 115–25.

James, D.E., Brown, R., Navarro, J., Pilch, P.F. (1988) Insulin-regulatable tissues express a unique insulin-sensitive glucose transport protein. Nature 333 (6169): 183–5.

James, D.E., Strube, M., Mueckler, M. (1989) Molecular cloning and characterization of an insulin-regulatable glucose transporter. Nature 338 (6210) : 83–87.

Merkens, J dan J.F. Mohede. (1941) Sumbangan pengetahuan tentang ayam Kedu. Terjemahan karangan mengenai ayam Kedu dan itik di Indonesia. LIPI. Jakarta.

Muryanto dan Subiharta, (1989) Pertumbuhan dan produksi telur ayam Kedu hitam yang dipelihara secara intensif. Pros. Seminar Hasil-hasil Penelitian. Fakultas Peternakan U.G.M. Yogyokarta

Patel, S.S., Udayabanu, M. (2014). Urtica dioica extract attenuates depressive like behavior and associative memory dysfunction in dexamethasone induced diabetic mice. Metab. Brain Dis. 29 (1) : 121–130.

Piroli,, G.G., Grillo, C.A., Reznikov, L.R., Adams, S., McEwen, B.S., Charron, M.J., Reagan, L.P. (2007) Corticosterone impairs insulin-stimulated translocation of GLUT4 in the rat hippocampus. Neuroendocrinology 85 (2 ): 71–80.

Sheperd, P.R., Kahn, B.B. (1999) Glucose Transporter and Insulin Action. N. England. J. Med. 341 (4) : 248-257.

Shulman, G. I. (2000) Cellular Mechanisms of Insulin Resistance. J. Clin. Invest. 106 (2). 106 (2 ): 171-176.

Tartaglia, L., dan Waugh, A. (2005) Veterinary Physiology and Applied Anatomy. Elsevier. Toronto

Watson, R.T., Kanzaki, M., Pessin, J.E. (2004) Regulated membrane trafficking of the insulin-responsive glucose transporter 4 in adipocytes. Endocrine Reviews 25 (2): 177–204. doi:10.1210/er.2003-0011.

DOI: https://doi.org/10.22146/jsv.34698