Perkembangan Resistensi Escherichia coli terhadap Oksitetrasiklin

Soedarmanto Indarjulianto(1), Alfarisa Nururrozi(2), Yanuartono Yanuartono(3), Rusmihayati Rusmihayati(4), Sitarina Widyarini(5*), Puspa Wikan Sari(6), Vembriarto Jati Pramono(7)

(1) Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
(2) Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
(3) Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
(4) Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
(5) Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
(6) Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
(7) Direktorat Kesehatan Hewan, Direktorat Jenderal Peternakan dan Kesehatan Hewan
(*) Corresponding Author


Antimicrobial resistance is amongst the primary concern in the field of veterinary medicine worldwide. The research was conducted to evaluate the resistance of Escherichia coli due to inappropriate antibiotic treatment. Escherichia coli in this research was cultured in vitro using 5-ml Tryptone Soya Broth (TSB), which was mixed with an antibiotic, then was incubated at 37 0C for four days (stage I). Subsequently, a part of the culture was transferred  into another 5-ml TSB and incubated at 37 0C for four days (stage II). This procedure was undertaken continuously for stage III, IV, V,and VI (until day 24). Antibiotics in this research was oxytetracycline with three various doses, which are lower dose (1 mg/ml), normal dose (2 mg/ml), and higher dose (8 mg/ml). The development of the antrimocobial resistance was evaluated every four days, using disk diffusion method and the data were analysed descriptively. The results showed that the normal and higher doses of oxytetracycline has the same rate (day 16) in causing E. coli resistant to oxytetracycline. Therefore, the treatment of oxytetracycline with a normal and higher doses continuously could accelerate the emergence of antimicrobial resistance in Escherichia coli.


Antibiotic resistance; Escherichia coli; oxytetracycline

Full Text:



Arias, C.A. and Murray, B.E. 2009. Antibiotic-Resistant Bugs in the 21st Century A Clinical Super-Challenge. N. Engl. J. Med. 360; 5: 349-443.

Bennett, PM. 2008. Plasmid encoded antibiotic resistance: acquisition and transfer of antibiotic resistance genes in bacteria (Review). British J. of Pharmacol. 153: S347–S357

Bradbury, W.C. and Munroe, D.L.G.. 1985. Occurance of plasmids and antibiotic resistance among Campylobacter jejuni and Campylobacter coli isolated from healthy and diarheic animals. J. Clin. Microbiol. 22: 339 –346.

Butaye, P., Deviase, L.A. and Hasebrouck, F. 2003. Antimicrobial Growth Promoters Used in Animal Feed: Effects of Less Well Known Antibiotics on Gram-Positive. Bact. Clin. Microbiol. Rev. 16 (2): 175 – 188.

CLSI. Clinical and Laboratory Standards Institute. 2010. Performance Standards for Antimicrobial Susceptibility Testing, Twentieth Informational Supplement: Supplement M100-S20, Clinical and Laboratory Standards Institute, Wayne, Pa, USA.

Dziva, F. and Stevens, MP. 2008. Colibacillosis in poultry: unravelling the molecular basis of virulence of avian pathogenic Escherichia coli in their natural hosts. Avian Pathol. 37(4):355-366.

Hintono, A., Astuti, M., Wuryastuti, H. dan Rahayu, ES. 2007. Pengaruh Peningkatan Zat Besi Ransum dan Pemberian Oksitetrasiklin pada Ayam Terhadap Kandungan Residu Oksitetrasiklin Dan Aktivitasnya Dalam Telur. J. Indon. Trop. Anim. Agric. 32 (4): 245-250.

Jelena. P., Baltic, M. Cupic, V. Stefanovic, S. and Dragica, S. 2006. Residues of enrofloxacin and its metabolite ciprofloxacin in broiler chickens. Acta Vet. (Beograd). 56: 497-506.

Jetacar (Joint Expert Advisory Committee On Antibiotic Resistance) Australia. 1999. The use Antibiotic in Food Producing Animals: Antibiotic resistance Bacteria in Animals and humans. Commonwealth of Australia.

Lee, M.D. and Lawrence, H.A. 1998. Colibacillosis. In A Laboratory Manual For the isolation an identification of avian pathogen. American Association of Avian Pathologist. Fourth Ed. Pennsylvania: pp: 14−16.

Lolo, M., Pedreira, S. Miranda, J.M., Vázquez, B.I., Franco, C.M., Cepeda, A. and Fente, C. 2006. Effect of cooking on enrofloxacin residues in chicken tissue. Food Add. Contam. 23: 988-993.

Mcewen, S.A. and Fedorka-Cray, P.J. 2002. Antimicrobial Use and Resistance in Animals. Clin. Infect. Dis. 34 (Suppl 3): 93 – 106.

Peleg, A.Y. and Hooper, D.C. 2010. Current Concepts, Hospital-Acquired Infections Due to Gram-Negative Bacteria. N. Engl. J. Med. 362:1804-1013.

Plumb, D.C. 1999. Veterinary Drug Handbook. IowaState University Press, Ames.

Quinn, P.J., Markey, B.K., Leonard, F.C., FitzPatrick, E.S., Fanning, S. and Hartigan, P.J. 2011. Veterinary Microbiology and Microbial Disease. Wiley-Blackwell, 2nd Ed.

Soedarmanto, I., Kanbar, T., Ülbegi-Mohyla, H., Hijazin, M., Alber, J., Lämmler, C., Akineden, O., Weiss, W., Moritz, A., and Zschöck, M. 2011. Genetic relatedness of methicillin-resistant Staphylococcus pseudintermedius (MRSP) isolated from a dog and the dog owner. Res. Vet. Sci. 91, Pp e-25-e27.

Soedarmanto, I., Schwarz, S., Liebisch, B., Lämmler, C. 1995. Tetracycline resistance determinants among streptococci of serological group G and L. Vet Microbiol. 45(4):331-337.

Stitzel, R. E and Craig, C. R. 2005. Modern Pharmacology. IowaState University Press, Ames.

Tadesse, D. A., Zhao, S., Tong, E., Ayers, S., Singh, A., Bartholomew, M. J. and McDermott, P. F. 2012. Antimicrobial Drug Resistance in Escherichia coli from Humans and Food Animals, United States, 1950–2002. Emerging Infectious Diseases, 18(5): 741-749.

Van Den Bogaard and Stobberingh, A.E. 1999. Antibiotic usage in animals: impact on bacterial resistance and public health. Drugs. 58(4): 589 – 607.

WHO. 1997. The medical impact of the use of antimicrobials in food animals: report and proceedings of a WHO meeting. 13 – 17 October. Berlin. WHO. Geneva. 28.


Article Metrics

Abstract views : 2909 | views : 2681


  • There are currently no refbacks.

Copyright (c) 2018 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861


HP. 0895363078367

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


web stats View My Stats