Daya Anthelmintik Infusa Biji Buah Pinang (Areca catechu) Terhadap Cacing Ascaridia galli Secara in Vitro

Wida Wahidah Mubarokah(1*), Wisnu Nurcahyo(2), Kurniasih Kurniasih(3)

(1) Politeknik Pembangunan Pertanian Yogyakarta Magelang
(2) Fakultas Kedokteran Hewan Universitas Gadjah Mada, Yogyakarta
(3) Fakultas Kedokteran Hewan Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author


A. galli infection caused high economic losses and decreased the amount and quality of egg production. The existence of anthelmintic resistance lead to the development of research on alternative treatments for A. galli. This aim of the research was to know LC50 of Areca catechu on infestation of A. galli. In this research A. galli was taken from a chicken slaughterhouse in Terban, Yogyakarta, and was divided into 8 groups for in vitro research. Group I was treated with an 10% infusion of A. catechu; group II was treated with 12.5% infusion of A. catechu; group III was treated with a 15% infusion of A. catechu; group IV was treated with a 17.5% infusion of A. catechu; group V was treated with 20% infusion of A. catechu; group VI was treated with a 22.5% infusion of A. catechu; group VII was treated with 25% A. catechu infusion and group VIII as negative control (0.9% NaCl). A. galli mortality is recorded every one hour until worm mortality is 100%. The results were then analyzed using the Reed and Muench method. Based on the in vitro test the infusion of Areca catechu against Ascaridia galli can cause death in worms with an LC 50 calculation of 21. 18%.


Areca cathecu; Ascaridia galli; LC50

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Alrubaie, A.L. 2015. Effect of alchoholic extract of Curcuma longa on Ascaridia infestation affecting chickens. Indian J. Exp. Biol., 53: 452-456.

Alvarez, L. I., Mottier, M. L. and Lanusse, C. E. 2007. Drug transfer into target helminths parasites. Trends Parasitol.,23: 97-104.

Amudhan, M. S., Begum, V. H. and Hebbar, K. B. 2012. A review on phytochemical and pharmacological potential of areca catechu l. seed. International Journal of Pharmaceutical Sciences and Research Vol 3. 4151–4156

Butaye, P., Devriese, L. A. & Haesebrouck, F. 2003. Antimicrobial growth promoters used in animal feed: Effects of less well known antibiotics on gram positive bacteria. Clinical Microbiology Reviews, 16, 175-188

Darmawi, D., Balqis, U., Hambal, M., Tiuria, R., Frengki. and Priosoeryanto, B.P. 2013. Mucosal mast cell response in jejunum of Ascaridia galli-infected laying hens. Media Peternak, 36(2): 113-119.

Das, G., Kaufmann, F., Abel, H. and Gauly, M. 2010. Effect of extra dietary lysine in Ascaridia galli-infected grower layers. Vet. Parasitol., 170: 238-243.

Febriani, Y., Hidayat, S., dan Seftiana, S. 2014. Aktivitas Anti Cacing Ekstrak Etanol Biji Pinang (Areca catechu) terhadap Ascaridia galli. Indonesian Journal of Pharmaceutical Science and Technology Vol.,III No.2. 1– 6

Hassanain, M.A., Rahman, E.H.A. and Khalil, F.A.M. 2009. New scanning electron microscopy look of Ascaridia galli (Schrank 1788) adult worm and its biological control. Res. J. Parasitol., 4: 1-11.

Iqbal, Z., Akhtar, M.S., Sindhu, Z., khan, M.N. and Jabbar, A. 2003. Review Herbal Dewormers in Livestock - A Traditional Therapy. International Journal of Agriculture & Biology :199-206

Kaplan, R.M. 2004. Drug Resistance in Nematodes of Veterinary Importance. A Status Report.Trends Parasitol., 20:477-481.

Kuchai, J.A., Ahmad, F., Chishti, M.Z., Tak, H., Ahmad, J.A.S. and Razool, M. 2012. A study on morphol­ogy and morphometry of Haemonchus contortus. Pak. J. Zool., 44(6): 1737-1741.

Lalchhandama, K., Roy, B. and Dutta, B.K. 2009. Anthelmintic activity of Acacia oxyphylla stem bark against Ascaridia galli. Pharm. Biol., 47(7): 578-583.

Lorent, J.H., Quetin-Leclercq, J. and Mingeot-Leclercq, M.P. 2014. The amphiphilic nature of saponins and their effects on artificial and biological membranes and potential consequences for red blood and cancer cells. Org. Biomol. Chem., 12: 8803-8822.

Martinez-Ortiz-de-Montellano, C., Arroyo-Lopez, C., Fourquaux, I., Torres-Acosta, J.F.J., Sandoval-Castro, C.A. and Hoste, H. 2013. Scanning electron microscopy of Haemonchus contortus exposed to tannin-rich plants under in vivo and in vitro conditions. Exp. Parasitol., 133(3): 281-286.

Max, R.A., Dawson, J.M., Wakelin, D., Buttery, P.J., Kimambo, A.E., Kassuku, A.A. dan Mtenga, L.A. 2002. Effect of Condensed Tanin Extracts on Gastrointestinal Nematodes of Small Ruminants. In: Proceedings of the Second Dfid Livestock Production Programme Link Project (R7798) Workshop for Smallstock Holders. Sokoine University of Agriculture, Morogoro, Tanzania, 71:195-207

Nnadi, P. A. and George, S. O. 2010. Research Article A Cross-Sectional Survey on Parasites of Chickens in Selected Villages in the Subhumid Zones of South-Eastern Nigeria. Journal of Parasitology Research., Vol.1 :1- 5

Nyambuya, T., Mautsa, R. and Mukanganyama, S. 2017. Alkaloid extracts from Combretum zeyheri inhibit the growth of Mycobacterium smegmatis. BMC Compl. Altern. Med., 17(124): 1-11.

Oliveira-Menezes, A., Lins, R., Norões, J., Dreyer, G. and Lanfredi, R.M. 2007. Comparative analysis of a chemotherapy effecton the cuticular surface of Wuchereria bancrofti adult worms in vivo. Parasitol. Res., 101: 1311-1317.

Ozaraga, B., Sylvia, M.A., Ozaraga,I. and Barrios, M.B. 2015. Ethnobotanical Dewormer Composition for Free Range Native Chickens. Mindanao Journal of Science and Technology., vol.13 :12-19

Reed, L.J. and Muench, H. 1938. A Simple Method of Estimating Fifty Per Cent Endpoints. The American Journal of Hygiene., 27(3): 493-497.

Rivera, K.C., Cortes, J.A.V., Lara, A.A., and Grandvallet, L.A.L. 2016. A Diagnostic Survey of Gastroenteric Helminths in Backyard Poultry of a Rural Vilage in Mexican TroPics. APRN Journal of Agricultural and Biological Science., Vol 11. No 12: 46- 469

Robinson, M.W., McFerran, N., Trudgent, A., Hoey, L. and Fairweather, I. 2004. A possible model of benzimidazole binding to ß-tubulin disclosed by invoking an inter-domain movement. J. Mol. Graph. Model., 23: 275-284.

Roy, B., Dasgupta, S., Manivel, V., Parameswaran, P.S. and Giri, B.R. 2012. Surface topographical and ultrastructural alterations of Raillietina echinobothrida and Ascaridia galli induced by a compound isolated from Acacia oxyphylla. Vet. Parasitol., 185: 322-326

Salam, S.T. 2015. Research article ascariasis in backyard chicken-prevalence, pathology and control. Int. J. Recent Sci. Res., 6(4): 3361-3365.

Sambodo, P., Prastowo, J., Kurniasih, K. and Indarjulianto, S. 2018. In vitro potential anthelmintic activity of Biophytum petersianum on Haemonchus contortus. Vet. World., 11(1): 1-4.

Susanti, A.E., dan Prabowo, A. 2014. Potensi Pinang (Areca catechu) sebagai Antelmintik untuk Ternak. Prosiding Seminar Nasional Pertanian Ramah Lingkungan Mendukung Bioindustri di Lahan Sub Optimal Palembang, 16 September 2014

Teixeira, M., Monteiro, J. P., Catenacci, L. S., Rodrigues, M. L. A., and Sato, M.CB. 2012. Ascaridiasis in Peafowl Pavo Cristatus (Phasianidae) Due to Ascaridiagalli Schrank. Journal of Zoo and Wildlife Medicine 43 (3). 585-587

Tiwow, D., Bodhi W. & Kojong, N.S. 2013.Uji Efek Antelmintik Ekstrak Etanol Biji Pinang (Areca catechu) Terhadap Ascaris lumbricoides dan Ascaridia galli Secara In Vitro. Pharmacon, 76-80.

Widiarso B.P., Kurniasih K, Prastowo, J., Nurcahyo, W. 2018. Morphology and morphometry of Haemonchus contortus exposed to Gigantochloa apus crude aqueous extract, Veterinary World, 11(7): 921-925.

Zhong, R.Z., Sun, H.X., Liu, H.W. and Zhou, D.W. 2014. Effects of tannin acid on Haemonchus contortus larvae viability and immune responses of sheep white blood cells in vitro. Parasite Immunol., 36: 100-106.

Zalizar, L., Satrija, F., Tiuria, R., dan Astuti, D.A. 2006. Dampak Infeksi Ascaridia galli terhadap Gambaran Histopatologi dan Luas Permukaan Vili Usus. JITV Vol. 11 No. 3.222- 228


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