ISOLASI, IDENTIFIKASI, DAN SEROTYPING Avibacterium paragallinarum DARI AYAM PETELUR KOMERSIAL YANG MENUNJUKKAN GEJALA SNOT

https://doi.org/10.22146/jsv.40375

Elisabet Tangkonda(1*), Charles Rangga Tabbu(2), Agnesia Endang Tri Hastuti Wahyuni(3)

(1) Fakultas Kedokteran Hewan Universitas Nusa Cendana Kupang
(2) Departemen Patologi Fakultas Kedokteran Hewan Universitas Gadjah Mada
(3) Departemen Mikrobiologi Fakultas Kedokteran Hewan Universitas Gadjah Mada
(*) Corresponding Author

Abstract


ABSTRAK

 

            Infectious coryza (snot) adalah salah satu penyakit pernapasan atas pada breeder, layer dan broiler yang disebabkan oleh Avibacterium paragallinarum (Av. paragallinarum). Penyakit ini sangat merugikan karena mengakibatkan penurunan produksi telur dan  morbiditas yang tinggi. Kejadian snot di Indonesia sampai sekarang masih terus dilaporkan. Pemberian vaksinasi adalah salah satu tindakan pencegahan terbaik, namun laporan tentang Av. paragallinarum dan jenis serotipe yang ada di lapangan masih sedikit, sehingga kesesuaian antara jenis serotipe Av. paragallinarum di lapangan dengan yang digunakan untuk vaksinasi belum diketahui. Av. paragallinarum memiliki strain dengan antigenisitas yang berbeda dan hingga saat ini diketahui tiga serotipe yaitu serotipe A, B dan C. Serotipe A dan C bersifat patogen sedangkan serotipe B kurang patogen. Tujuan penelitian ini adalah untuk mengisolasi, mengidentifikasi dan menentukan serotipe Av. paragallinarum dari ayam petelur komersial yang menunjukkan gejala snot. Sampel diambil dari ayam yang menunjukkan gejala snot (eksudat berbau busuk dari mata dan hidung, kebengkakan infra orbital dan pial, konjungtivitis, dan adanya suara ngorok halus) dari beberapa peternakan layer. Sampel dikultur pada media agar coklat kemudian diinkubasi dalam candle jar  pada suhu 37 ᴼC selama 18-24 jam. Morfologi koloni dan sel bakteri diamati dan dilakukan uji biokimia (katalase, oksidase, urease, indol, dan fermentasi karbohidrat) pada koloni yang diduga Av. paragallinarum. Uji serotipe dilakukan dengan metode plate agglutination test (PAT). Penelitian ini mendapat hasil 4 isolat Av. paragallianrum dengan 2 isolat merupakan serotipe B dan 2 lainnya serotipe C.

 

ISOLATION, IDENTIFICATION, AND SEROTYPING Avibacterium paragallinarum FROM COMERCIAL LAYER WITH SNOT SYMPTOMS

 

 

ABSTRACT

 

            Infectious coryza (snot) is one of acute respiratory disease in breeders, layers and broilers caused by Avibacterium paragallinarum (Av. paragallinarum). This disease is very harmful because its cause decrease in egg production and high morbidity. Snot incident in Indonesia still be reported. Vaccination is one of the best preventive measures, but reports about Av. paragallinarum serotype at field so lack, so that the correspondence between serotype Av. paragallinarum in the field to those used for vaccination is unknown. Av. paragallinarum has strains with different antigenicity and until now known three serotypes there are serotypes A, B and C. Serotypes A and C are pathogenic then serotypes B. The purpose of this study was to isolate, identify and to determine the serotype of Av. paragallinarum from commercial layer that showing symptoms of snot. Samples taken from layer which showed symptoms of snot (nasal foul smelling exudate, infra-orbital and wattle swelling, conjunctivitis, and snoring) from some layer farms. Samples were cultured on chocolate agar and then incubated in a candle jar at 37 ᴼC for 18-24 hours. Bacteria colony and cell morphology was observed and performed biochemical tests (catalase, oxidase, urease, indole, and fermentation of carbohydrates) in suspected colonies. Serotypes test was conducted using plate agglutination test (PAT). This study revealed 4 isolates Av. paragallinarum with 2 isolates are serotype B and 2 other are serotype C.

 


Keywords


infectious coryza, Avibacterium paragallinarum, isolation, identification, serotype



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DOI: https://doi.org/10.22146/jsv.40375

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