Keanekaragaman Kapang Patogen dan Non Patogen pada Imago, Kokon, dan Larva Instar Keenam Ulat Sutera Liar Attacus atlas (Lepidoptera: Saturniidae)

Agustin Indrawati; Damiana Rita Ekastuti; Erdina Pangestika; Reinilda Alwina

doi:10.22146/jsv.48022

Keanekaragaman Kapang Patogen dan Non Patogen pada Imago, Kokon, dan Larva Instar Keenam Ulat Sutera Liar Attacus atlas (Lepidoptera: Saturniidae)

https://doi.org/10.22146/jsv.48022

Agustin Indrawati^(1*), Damiana Rita Ekastuti⁽²⁾, Erdina Pangestika⁽³⁾, Reinilda Alwina⁽⁴⁾

(1) Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan Institut Pertanian Bogor
(2) Departemen Anatomi, Fisiologi, dan Farmakologi, Fakultas Kedokteran Hewan Institut Pertanian Bogor
(3) Fakultas Kedokteran Hewan Institut Pertanian Bogor
(4) Fakultas Kedokteran Hewan Institut Pertanian Bogor
(*) Corresponding Author

Abstract

Attacus atlas is one of several mould species in Indonesia known as kupu-kupu gajah. Information about variety of mould is rarely known. The purpose of this research was to obtain data about variety of pathogenic or non pathogenic mould at imago,cocoon, and sixth larvae phase of wild silkworm A. atlas. Mould was isolated from cocoon, integument, alimentary duct and reproduction duct of imago, trachea, midgut and hindgut, also haemolymph of larvae. Isolated mould was cultured on potato dextrose agar. Isolated mould from cocoon and imago was identified by macroscopic and microscopic observation. The results showed that there were two kind of moulds from cocoon which were Fusarium oxysporum and Aspergillus flavus. There were four kind of moulds from imago A. atlas which were Aspergillus flavus, Aspergillus versicolor, Aspergillus fumigatus, Fusarium dimerum, and Aspergillus sp.There were three kind of moulds from sixth larvae which were Aspergillus fumigatus, Aspergillus flavus, and Fusarium dimerum. The mould which has opportunistic pathogenic for Attacus atlas were Fusarium oxysporum, Aspergillus flavus, Aspergillus fumigatus and Fusarium dimerum.

Keywords

Attacus atlas; imago; cocoon; larvae; mould

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References

Abad, A., Fernández-Molina,J.V., Bikandi, J., Ramírez, A., Margareto,J.,Sendino, J., Hernando,F.L., Pontón,J., Garaizar,J.,and Rementeria, A. 2010. What makes Aspergillus fumigatus a successful pathogen: Genes and molecules involved in invasive aspergillosis. Rev Iberoam Micol. 27(4):155-182.

Arsy, R. 2014. Isolasi dan identifikasi bakteri pada jaringan lemak imago betina ulat sutera liar Attacus atlas L. (Lepidoptera: Saturniidae) [skripsi]. Bogor (ID): Institut Pertanian Bogor.

Awan, A. 2007. Domestikasi Ulat Sutera Liar Attacus atlas (Lepidoptera : Saturniidae) dalam usaha meningkatkan persuteraan nasional [disertasi]. Bogor (ID): Sekolah Pascasarjana Institut Pertanian Bogor.

Azlina, M.D., Long, K., and Ghazali, H.M. 2004. Improvement of the specific activity of the extracted mycelium-bound lipase of Aspergillus flavus. J Trop Agric Fd Sc..32(2):187–195.

Campbell, C.K., Johnson, E.M., Philpot, C.M., and Warnock, D.W. 1996. Identification of pathogenic fungi. London (GB): Public Health Lab Service.

Chapman, R.F. 1998. The Insects: Structure and Function. 4^th ed. Cambridge (GB): Cambridge Univ Pr.

Domsch, K.H., Gams, W., and Anderson, T. 2007. Compendioum of soil fungi. 2^nd ed. Eching(DE): IHW-Verlag.

Engelhart,S., Loock,A., Skutlarek, D.,Sagueski,H.,Lommel,A., Farber,H.,and Exner,M.2002. Occurence of toxigenic Aspergillus versicolor isolatess and Sterigmatocystin in carpet dust from damp indoor environments. App Environ Microbial.68(8):3886-3890

Esnawan, A.A. 2015. Isolasi dan identifikasi bakteri saluran reproduksi imago betina ulat sutera liar Attacus atlas L. (Lepidoptera: Saturniidae) [skripsi]. Bogor (ID): Institut Pertanian Bogor.

Faatih, M. 2005. Aktivitas antimikroba Attacus atlas L. J penelitian sain dan teknologi6(1):35–48.

Fajar, M.2015. Isolasi dan identifikasi bakteri kloaka imago betina ulat sutera liar Attacus atlas L. (Lepidoptera: Saturniidae) [skripsi]. Bogor (ID): Institut Pertanian Bogor.

Firáková,S., Šturdíková, M., Liptaj,T., Prónayová, N., Bezáková, L., and Proksa, B. 2008. Enniatins produced by Fusarium dimerum, an endophytic fungal strain. Pharmazie. 63:539–541.

Fisher, F., and Cook, N.B. 1998. Fundamentals of Diagnostic Mycology. Pennsylvania (US): WB Saunders.

Gandjar, I., Samson, R.A., Vermerulen, K.V.D.T., Oetari, A., dan Santoso, I. 1999. Pengenalan Kapang Tropik Umum. Jakarta (ID): Yayasan Obor Indonesia.

Guntoro, S. 1994. Budidaya Ulat Sutera. Yogyakarta (ID): Kanisius

Gupta, A., and Gopal, M. 2002. Aflatoxin production by Aspergillus flavus isolates pathogenic to coconut insect pests. J Microbial Biotechnol. 18:325-331.

Hee, R.C. 1998. Panduan Teknis Persuteraan Alam Indonesia. Surabaya (ID): PT Indojado Sutera Pratama, Silk Industri.

Heritage, J., Evans, G.V.,and Killington, R.A. 1996. Introductory Microbiology. Cambridge (GB): Cambridge Univ. Pr.

Hong, G., Li, Y., Jun, G., Ma, X., Wang, H.,and You, S. 2007. Morphological and molecular identification of Aspergillus versicolor D-1 with selective reduction ability..J. Tradi Med. 2(1):39-44.

Huh, H.J., Lee, J.H., Park, K.S., Jun ,T.G., Kang, I., Kim, Y., Ki, C.,and Lee, N.Y. 2013. A Case of Misidentification of Aspergillus versicolor Complex as a Scopulariopsos Species Isolated from a Homograft. Ann Clin Microbiol.16(2):105-109.

[IARC] International Agent Research Cancer. 2015. Agents Classified by the IARC Monographs Volume 1-112.

Istiqomah H. 2014. Eksplorasi potensi limbah imago ulat sutera liar Attacus atlas (Lepidoptera: Saturniidae) [skripsi]. Bogor (ID): Institut Pertanian Bogor.

Latgé, J.P. 1999. Aspergillus fumigatus and Aspergillosis. Clin Microbiol Rev 12(2):310–350.

Leger, R.J., Screen, S.E.,and Shams–pirzadeh, B. 2000. Lack of host specialization in Aspergillus flavus. Appl Environ Microbiol. 66(1):320-324.

Li, D.W. 2013. Laboratory Protocols in Fungal. Gupta VK, Tuohy MG, Ayyachamy M, Turner KM, O’Donovan A, editor. New York (US): Springer-Verlag.

Markey, B., Leonard, F., Archambault, C.A., Maguire, D.2013. Clinical Veterinary Microbiology. 2nd ed. Britania (GB): Mosby Elsevier.

Mulyani, N. 2008. Biologi Attacus atlas L. (Lepidoptera: Saturniidae) dengan pakan daun kaliki (Ricinus communis L.) dan jarak pagar (Jatropha curcas L.) di laboratorium [tesis]. Bogor (ID): Institut Pertanian Bogor

Novitasari, P. 2013. Isolasi dan identifikasi bakteri kinolitik penghambat pertumbuhan kapang patogen asal kokon Cricula trifenestrata [skripsi]. Bogor (ID): Institut Pertanian Bogor.

Nucci, M., and Anaissie, E. 2007. Fusarium Infections in Immunocompromised Patients. Clin Microbiol Rev.20(4):695-704.

O’Donnel, K., Humber, R.A., Geiser, D.M., Kang, S.C., Park, B.S., Robert ,V.A.R.G., Crous, P.W., Johnston, P.R., Aoki, T., and Rooney, A.P. 2012 Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM–ID and Fusarium MLST. Mycologia. 104(2):427–445.

Pandit, S., Lawrence, K., Singh, A., and Singh, S. 2014. Saccharification of Baggase, Paper, waste by cellulase produced from Aspergillus flavus. Int J Pure App Biosci. 2(3):16-22.

Pelizza, S.A., Stenglein, S.A., Cabello, M.N., Dinolfo, M.I., and Lange, C.E. 2011. First Record of Fusarium verticillioides as an entomopathogenic fungus on Grasshoppers. J Insect Sci. 11(70):1-8.

Rheeder, J.P., Marasas, W.F.O., and Vismer, H.F. 2002. Production of fumonisin analogs by Fusarium species. Appl Environ Microbiol. 68(5):2101–2105.

Schroers, H.J., O’Donnell, K., Lamprecht, S.C., Kammeyer, P.L., Johnson, S., Sutton, D.A., Rinaldi, M.G., Geiser, D.M., and Summerbell, R.C. 2009. Taxonomy and phylogeny of the Fusarium dimerum species group. Mycologiantoia. 101(1):44-70..

Scully, L.R.,and Bidochka, M.J. 2006. A cysteine/methionine auxotroph of the opportunistic fungus Aspergillus flavus is associated with host-range restriction: a model for emerging diseases. Microbiol. 152:223-232

Sun, B., and Liu, X. 2008. Occurrence and diversity of insect-associated fungi in natural soils in China. App Soil Ecology. 39:100-108.

Vismer, H.F., Marasas, W.F., Rheeder, J.P., and Joubert, J.J. 2002. Fusarium dimerum as a cause of human eye infections. Med Mycol.40:399–406.

DOI: https://doi.org/10.22146/jsv.48022