Swab Bukal Sebagai Bahan Sexing Piyikan Burung Kenari (Serinus canaria) dan Burung Merpati (Columba livia)

https://doi.org/10.22146/jsv.49032

Afif Muhammad Akrom(1), Soedarmanto Indarjulianto(2*), Yanuartono Yanuartono(3), Trini Susmiati(4), Alfarisa Nururrozi(5), Slamet Raharjo(6), Rief Ghulam Satria Permana(7), Yeremia Yobelanno Sitompul(8)

(1) Universitas Gadjah Mada
(2) Universitas Gadjah Mada
(3) Universitas Gadjah Mada
(4) Universitas Gadjah Mada
(5) Universitas Gadjah Mada
(6) Universitas Gadjah Mada
(7) Universitas Gadjah Mada
(8) Universitas Nusa Cendana
(*) Corresponding Author

Abstract


Teknik sexing pada burung secara molekuler dengan metode PCR telah banyak dikembangkan, tetapi sampel yang digunakan adalah darah dan bulu yang dianggap invasif. Penelitian ini bertujuan untuk mempelajari efisiensi sampel swab bukal sebagai sumber DNA dalam sexing dengan metode PCR. Penelitian ini menggunakan 10 ekor burung kenari (Serinus canaria) yang terdiri dari 6 ekor burung dewasa (3 jantan dan 3 betina) dan 4 ekor kenari piyikan (umur 14 – 18 hari) yang belum diketahui jenis kelaminnya serta 6 ekor merpati (Columba livia) dewasa (3 jantan dan 3 betina) dan 7 ekor merpati piyikan (umur 14 – 25 hari) yang belum diketahui jenis kelaminnya. Amplifikasi fragmen gen dilakukan menggunakan metode PCR dengan pasangan primer CHD1F/CHD1R.Hasil visualisasi produk PCR menunjukkan semua burung jantan dewasa menghasilkan satu band (± 500 bp), sedangkan burung betina dewasa menghasilkan dua band (± 500 bp dan ± 300 bp). Amplifikasi gen dari swab bukal burung kenari muda didapatkan 2 ekor jantan dan 2 ekor betina, sedangkan dari swab bukal burung merpati muda didapatkan 6 ekor jantan dan 1 ekor betina. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa sampel swab bukal terbukti efisien sebagai sumber DNA dalam sexing burung khususnya burung piyikan.

Keywords


sexing; PCR; swab bukal; burung Kenari; burung Merpati

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DOI: https://doi.org/10.22146/jsv.49032

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