Prevalence and risk factors of Cryptosporidium spp. on dairy farms in Bogor

Arifin Budiman Nugraha; Umi Cahyaningsih; Etih Sudarnika

doi:10.22146/jsv.55961

Prevalence and risk factors of Cryptosporidium spp. on dairy farms in Bogor

https://doi.org/10.22146/jsv.55961

Arifin Budiman Nugraha^(1*), Umi Cahyaningsih⁽²⁾, Etih Sudarnika⁽³⁾

(1) Laboratory of Protozoology Faculty of Veterinary Medicine IPB University, Bogor
(2) Laboratory of Protozoology Faculty of Veterinary Medicine IPB University, Bogor
(3) Veterinary Public Health Faculty of Veterinary Medicine IPB University, Bogor
(*) Corresponding Author

Abstract

Cryptosporidial infection is one of the most common causes of diarrhea in humans and livestock worldwide. This study was conducted to estimate the prevalence of Cryptosporidium infection and to identify potential risk factors associated with shedding of oocysts in Bogor. A total of 308 faecal samples were collected from 136 calves less than 6 months, 44 from those 6-12 months and 128 from those than 12 months. Data of factors potentially associated with the likelihood of Cryptosporidium spp. infection were recorded (i.e., enviromental status, size of herd, and herd management). Cryptosporidium spp. oocyst was identified by using modified acid fast (Ziehl Neelsen) staining technique and microscopically examined under 400x magnifition. Results showed that the prevalence of cryptosporidiosis in Bogor was 21.1% (CI 95%; 16.5%-25.6%). The highest prevalence was 29% (CI 95%; 26.8%-31.7%) in cattle aged less than 6 months. The oocysts abundance were around <5 oocysts per microscopy visual area. Data was analyzed using logistic regression models. Statistical analysis showed that there were association between cryptosporidiosis and calves aged less than 6 months with an odds ratio (OR) of 2.7 (CI 95%; 1.5-5.2) times compared with cattle aged more than 12 months.

Keywords

bogor; cryptosporidium spp.; dairy farm; prevalence; risk factors.

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References

Anderson, B.C., Hall, R.F. (1982). Cryptosporidial infection in Idaho dairy calves. J Am Vet Med Assoc. 181: 484-485. Anderson, Bruce, C. (1998). Cryptospoidiosis in bovine and human health. J dairy Sci. 81: 3036-3041. Artama, I.K. (2005). Cross Sectional Study of Cryptosporidiosis for Bali Cattle in Karang Asem Bali. Thesis. Faculty of Veterinary Medicine, IPB University. Atwill, E.R., Johnson, E.M., Pereira, M.G. (1999). Association of herd composition, stocking rate, and duration of calving season with fecal shedding of Cryptosporidium parvum oocysts in beef herds. J Am Vet Med Assoc. 215: 1833–1838. Ananta, M.S., Suharno, Hidayat, A., Makoto, M. (2014). Survey on gastrointestinal parasites and detection of Cryptosporidium spp. on cattle in West Java, Indonesia. Asian Pac J Trop Dis. 7: 197-201. Banda, Rosely, A.B., Nichols, A.M., Grimason, Smith, H.V.(2009). Cryptosporidium infection in non-human hosts in Malawi. Onderstepoort J Vet Res. 76: 363–375. Brook, E., Hart, A., French, N., Christley, R. (2008). Prevalence and risk factors for Cryptosporidium spp. Infection in young calves. Vet Parasitol. 152: 46-52. Castro, Hemida, J.A., Caroo, Corral, Gonzales, W, Mezo, M. (2006). Prevalence and intensityof infection of Cryptosporidium spp and Giardia duodenalis in dairy cattlein Galicia. J Vet Med. B. Infect Dis. Vet Public Health. 53: 244-246. Clark, D.P., and Sears, C.L. (1996). The Patoghenesis of cryptosporidiosis. Parasitol Today. 12: 221-225. Korich, D.G., Mead, J.R., Madore, M.S., Sinclair, N.A., Sterling, C.R. (1990). Effects of ozone, chlorine dioxide, chlorine, and monochloramine on Cryptosporidium parvum oocysts viability. Appl Environ Microbiol . 56: 1423-1428. Maldonado-Camargo, S., Atwill, E.R., Saltijeral-Oaxaca, J.A., Herrera-Alonso, L.C. (1998). Prevalence and risk factors for shedding of Cryptosporidium parvum in Holstein Freisian dairy calves in central Me´xico. Prev Vet Med. 36: 95–107. Naciri, M., Mancassola, R., Fort, G., Danneels, B., Verhaeghe, J. (2011). Efficacy of amine-based disinfectant KENO COX on the infectivity od Cryptosporidium parvum oocycts. Vet Parasitol. 179 : 43-49. Nydam, D.V., Mohammed, H.O. (2005). Quantitative risk assessment of Cryptosporidium species infection in dairy calves. J.dairy Sci. 88: 3932-3943. Ramirez, N.E., Ward, L.A., Sreevatsan, S. 2004. A review of the biology and epidemiology of cryptosporidiosis in humans and animals. Microbes and Infection. 6: 773-785. Rosiléia, M, De Quadros, Sandra, M.T., Marques, Camila, R., Amendoeira, Larissa, A., De Souza, Paula, R., Amendoeira, Carla, C. (2006). Comparison detection of Cryptosporidium oocysts by auramine and Ziehl Neelsen staining methods. Parasitol Latinoam. 61 :117–120. Sischo, W.M., Okhuyen, P.C., Atwill, E.R., Lanyon, L.E., George, J. (2000). Cryptosporidiaon dairy farms and the role these farms may have in contaminating surface water supplies in the northeastern United States. Prev Vet Med. 43: 253-267. Thrusfield M. (2005). Veterinary Epidemiology. 3rd ed. Iowa, United States of America. Thompson, S., Hamilton, C.A., Hope, J.C., Katzer, F., Mabbott, N.A., Morisson, L.J., Innes, E.A. (2017). Bovine cryptosporidiosis: impact, host-parasite interaction and control strategies. Vet Research. 48:42. Yoshinori, T., Panagiotis, K., Takenori, K., Hideyuki, N.H., Xuenan, X., Ikuo, I., Kozo, F., Eiki, T., Takeshi, M. (2000). Detection of Cryptosporidium parvum oocysts in enviromental water in Hokaido, Japan. J Vet Med. 63: 233-236.

DOI: https://doi.org/10.22146/jsv.55961