Swab Bukal Sebagai Bahan Sexing Piyikan Burung Kenari (Serinus canaria) dan Burung Merpati (Columba livia)

https://doi.org/10.22146/jsv.57364

Afif Muhammad Akrom(1*), Soedarmanto Indarjulianto(2), Yanuartono Yanuartono(3), Trini Susmiati(4), Alfarisa Nururrozi(5), Slamet Raharjo(6), Rief Ghulam Satria Permana(7), Yeremia Yobelanno Sitompul(8)

(1) Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(2) Departemen Ilmu Penyakit Dalam, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(3) Departemen Ilmu Penyakit Dalam, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(4) Departemen Biokimia, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(5) Departemen Ilmu Penyakit Dalam, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(6) 
(7) Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(8) Departemen Klinik, Reproduksi, Patologi, dan Nutrisi, Fakultas Kedokteran Hewan, Universitas Nusa Cendana, Kupang
(*) Corresponding Author

Abstract


Molecular sexing for bird with polymerase chain reaction-based method have been developed, but the samples used as a sources of DNA are blood and feathers which are considerably invasive. The aim of this study was to study the efficiency of buccal swabs as a resource of DNA for sexing canary bird and pigeon. This study used 10 canaries (Serinus canaria) consisting of 6 adult canaries (3 males and 3 females) and 4 young nestling canaries (14 - to 18-day old) and 6 adults (3 males and 3 females) pigeons (Columba livia) and 7 young nestling pigeons (14- to 25-day-old). All birds were taken their buccal swab samples, then DNA were extracted, mixed with PCR-mix to be amplified for sexing genes with CHD1F/CHD1R primer pairs. The amplification results showed that all of adult male birds produced single band (± 500 bp), whereas all of adult female birds produced double bands (± 500 bp and ± 300 bp). The PCR method for nestling canaries showed 2 males and 2 females. whereas nestling pigeons 6 males and 1 female. Based on this study it can be concluded that buccal swabs are efficient as a source of DNA for birds sexing especially young nestling birds. 


Keywords


sexing; PCR; buccal swab; pigeon; canary

Full Text:

PDF


References

Arima, H. and Ohnishi, N. (2006). Usefulness of avian buccal cells for molecular sexing. Ornithol Sci. 5: 139 – 143.

Asawakarn, S., Teeranuwat, I., Watcharaprapapong, N., Siriwatchaiporn, N., Somsai, P., Kuldee, M., Suriyaphol, G., and Dhitavat, S. (2018). Comparison of Dried Blood Spot, Buccal Swab, Cloacal Swab and Feces as DNA Sources toIdentify Avia Sexes by PCR. Thai J Vet Med. 48 (3): 325 – 330.

Birchard, G.F. and Deeming, D.C. (2015). Egg Allometry: Influences of hylogeny and the Altricial-Precocial Continuum. In Nests, Eggs, & Incubation New Ideas About Avian Reproduction. Deeming, D.C. and Reynolds, SJ. (Ed). Oxford University Press. Oxford

Bosnjak, J., Stevanov-Paviovic, M., Vucicevic, M., Stevanovic, J., Simeunovic. P., Resanovic, R. and Stanimirovic, Z. (2013). Feasibility of non-invasive molecular method for sexing of parrots. Pakistan J. Zool 45: 715 – 720

Bostwick, K. (2016). Feathers and Plumage. In Handbook of Bird Biology. Lovette, I.J. and Fitzpatrick, J.W. (Ed). 3rd ed. Cornell University, Oxford.

Brown, M.B., and Brown, C.R. (2009). Blood sampling reduces annual survival in Cliff Swallows (Petrochelidon pyrrhonota). The Auk 126:853–861.

Brooks, R., Williamson, J., Hensley, A., Butler, E., Touchton, G. and Smith, E. (2003). Buccal Cells as a Source of DNA for Comparative Animal Genomic Analysis. Biotechnol Lett. 25: 451 – 454.

Brubacker, J.L., Karouna-Renier, N.K., Chen, Y., Jenko, K., Sprague, D.T. and Henry, P.F.P. (2011). A Noninvasive, Direct Real-Time PCR Method for Sex Determination in Multiple Avian Spesies. Molecular Ecology Resources. 11: 415 – 417.

Cakmak, E., Peksen, C.A. and Bilgin, C.C. (2017). Comparison of Three Different Primer Sets for Sexing Birds. J Vet Diag Invest. 29: 5963

Eiben, K., Fay, R., Jung, A., Rasmussen, A., and Russell, J. (2017). Sex Determination of The Boreal Owl (Aegolius funereus) Using Buccal Swabs and Improved Molecular Techniques. J. Raptor Res. 51 (1): 68 – 71.

Jepson, P. (2010). Towards and Indonesian Bird Conservation Ethos: Reflections From a Study of Birds-keeping in The Cities of Java and Bali. In Ethno-ornithology: Birds, Indigenous People, Culture and Society.

Tidemann, S. and Gosler, A. (Ed). Earthscan, London. Lee, J.C., Tsai, L., Hwa, P., Chan, C., Huang, A., Chin, S., Wang, L., Lin, J., Linacre, A. and Hsies, H. (2010). A Novel Strategy for Avian Spesies and Gender Identification using the CHD Gene. Molecullar and Cellular Probes. 24: 27 – 31.

McDonald, P. and Griffith, S.C. (2011) To Pluck or not Pluck: The Hidden Ethical and Scientific Cost of Relying on Feathers as a Primary Source of DNA. J. Avian Biol. 42: 197 – 203.

Meldgaard, M., Bollen, P.J.A. and Finsen, B. (2004). Non-invasive Method for Sampling and Extraction of Mouse DNA for PCR. Lab Anim. 38: 413 – 417.

Morinha, F., Cabral, J.A. and Bastos, E. (2012). Molecular sexing of birds: a comparative review of polymerase chain reaction (PCR)based methods. Theriogenology. 78: 703– 714.

Price, T. and Birch, G.L. (1996). Repeated Evolution Of Sexual Color Dimorphism in Passerine Birds. The Auk. 113: 842–848.

Vilstrup, J.T., Mullins, T.D., Miller, M.P., Mc Dearman, D., Jeffrey, R., Walters, J.R., Haig, S.M. (2018). A simplified field protocol for genetic sampling of birds using buccal swabs. The Wilson J. of Ornithology, 130 (1): 326-334.

Voss, M., Shutler, D. and Werner, J. (2010) A Hard Look at Blood Sampling of Birds. The Auk. 127 (3): 704 – 708.

Vucicevic, M., Stevanov-Pavlovic, M., Stevanovic, J., Bosnjak, J., Gajic, B., Aleksic, N. and Stanimirovic, Z. (2012). Sex Dtermination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing. Zoo Biology: 1-13

Wellbrock AH, Bauch C, Rozman J. and Witte K. (2012) Buccal swabs as a reliable source of DNA for sexing young and adult Common Swift (Apus apus). J Ornithol. 153: 991 – 994.

Winkler, D.W. (2016). Breeding Biology of Birds. In Handbook of Bird Biology. Lovette, I.J. and Fitzpatrick, J.W. (Ed). 3rd ed. Cornell University, Oxford.

Yannic, G., Sermier, R., Aebischer, A., Gavrilo, M.v., Gilg, O., Miljeteig, C., Sabard, B., Strom, H., Pouive, E. and Broquet, T. (2011). Description of Microsatellite Marker and Genotyping Performances using Feathers and Buccal Swabs fo Ivory Gull (Pagophila eburnean). Mol Ecol Resour. 11: 877 – 889.



DOI: https://doi.org/10.22146/jsv.57364

Article Metrics

Abstract views : 380 | views : 608

Refbacks

  • There are currently no refbacks.




Copyright (c) 2020 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

    CrossrefROADCOREProduct DetailsDESKRIPSI GAMBAR


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

HP. 0895363078367

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats