Swab Bukal Sebagai Bahan Sexing Piyikan Burung Kenari (Serinus canaria) dan Burung Merpati (Columba livia)

Afif Muhammad Akrom; Soedarmanto Indarjulianto; Yanuartono Yanuartono; Trini Susmiati; Alfarisa Nururrozi; Slamet Raharjo; Rief Ghulam Satria Permana; Yeremia Yobelanno Sitompul

doi:10.22146/jsv.57364

Swab Bukal Sebagai Bahan Sexing Piyikan Burung Kenari (Serinus canaria) dan Burung Merpati (Columba livia)

https://doi.org/10.22146/jsv.57364

Afif Muhammad Akrom^(1*), Soedarmanto Indarjulianto⁽²⁾, Yanuartono Yanuartono⁽³⁾, Trini Susmiati⁽⁴⁾, Alfarisa Nururrozi⁽⁵⁾, Slamet Raharjo⁽⁶⁾, Rief Ghulam Satria Permana⁽⁷⁾, Yeremia Yobelanno Sitompul⁽⁸⁾

(1) Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(2) Departemen Ilmu Penyakit Dalam, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(3) Departemen Ilmu Penyakit Dalam, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(4) Departemen Biokimia, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(5) Departemen Ilmu Penyakit Dalam, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(6)
(7) Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(8) Departemen Klinik, Reproduksi, Patologi, dan Nutrisi, Fakultas Kedokteran Hewan, Universitas Nusa Cendana, Kupang
(*) Corresponding Author

Abstract

Molecular sexing for bird with polymerase chain reaction-based method have been developed, but the samples used as a sources of DNA are blood and feathers which are considerably invasive. The aim of this study was to study the efficiency of buccal swabs as a resource of DNA for sexing canary bird and pigeon. This study used 10 canaries (Serinus canaria) consisting of 6 adult canaries (3 males and 3 females) and 4 young nestling canaries (14 - to 18-day old) and 6 adults (3 males and 3 females) pigeons (Columba livia) and 7 young nestling pigeons (14- to 25-day-old). All birds were taken their buccal swab samples, then DNA were extracted, mixed with PCR-mix to be amplified for sexing genes with CHD1F/CHD1R primer pairs. The amplification results showed that all of adult male birds produced single band (± 500 bp), whereas all of adult female birds produced double bands (± 500 bp and ± 300 bp). The PCR method for nestling canaries showed 2 males and 2 females. whereas nestling pigeons 6 males and 1 female. Based on this study it can be concluded that buccal swabs are efficient as a source of DNA for birds sexing especially young nestling birds.

Keywords

sexing; PCR; buccal swab; pigeon; canary

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DOI: https://doi.org/10.22146/jsv.57364