Pengembangan Media Transpor untuk Koleksi Sampel Preputium, untuk deteksi Bovine Genital Campylobacteriosis

Apris Beniawan; Agustin Indrawati; Fachriyan Hasmi Pasaribu

doi:10.22146/jsv.60148

Pengembangan Media Transpor untuk Koleksi Sampel Preputium, untuk deteksi Bovine Genital Campylobacteriosis

https://doi.org/10.22146/jsv.60148

Apris Beniawan^(1*), Agustin Indrawati⁽²⁾, Fachriyan Hasmi Pasaribu⁽³⁾

(1) Badan Karantina Pertanian, Kementerian Pertanian, Jakarta Selatan
(2) Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Dramaga, Bogor
(3) Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Dramaga, Bogor
(*) Corresponding Author

Abstract

Campylobacter fetus subsp. Venerealis (Cfv) is bacteria causing contagious genital diseases in cows called Bovine Genital Campylobacteriosis (BGC) or vibriosis. Isolation of Cfv is difficult, because the bacteria are fragile and need specific nutrients and oxygen (5-10%). The transport media is very important to maintain Cfv survival before culturing in laboratory. The aim of this study was to modify a new transport media as an alternative media for Cfv. Modification media capability was compared to Weybridge media, and Phosphate Buffered Saline (PBS). All transport media was contaminated by Cfv with concentrations of 105, 104, 103, 102, 101 (CFU/ml), and was stored for <6, 24, 48, 72, and 96 hours in each transport medium before culturing on blood agar, all in triplicate. The quality of transport media was analyzed based on bacterial growth on blood agar. PCR test was used as a confirmatory test of growing bacteria cultured on blood agar. Based on culture results, Cfv stored in
three transport mediums for <6 hours, Cfv grew on blood agar from all concentration levels provided. Cfv stored for 24, 48, 72 and 96 hours on PBS did not grow, whereas on modification and Weybridge media, the bacteria could grow, and enrichment occurs at all concentration levels given. This study indicated the media modification can be used as an alternative transport medium for Cfv bacteria.

Keywords

Campylobacter fetus subs. venerealis (Cfv); culture; transport Media

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DOI: https://doi.org/10.22146/jsv.60148