In Vitro Anthelmintic Areca catechu Crude Aqueous Extract Against Haemonchus contortus in The Sheep

Annisa Putri Cahyani; Budi Purwo Widiarso; Supriyanto -; Wida Wahidah Mubarokah

doi:10.22146/jsv.70399

In Vitro Anthelmintic Areca catechu Crude Aqueous Extract Against Haemonchus contortus in The Sheep

https://doi.org/10.22146/jsv.70399

Annisa Putri Cahyani⁽¹⁾, Budi Purwo Widiarso⁽²⁾, Supriyanto -⁽³⁾, Wida Wahidah Mubarokah^(4*)

(1) Politeknik Pembangunan Pertanian Yogyakarta Magelang
(2) Politeknik Pembangunan Pertanian Yogyakarta Magelang
(3) Politeknik Pembangunan Pertanian Yogyakarta Magelang
(4) Politeknik Pembangunan Pertanian Yogyakarta Magelang
(*) Corresponding Author

Abstract

Haemonchosis is a gastrointestinal parasitic disease in sheep caused by Haemonchus contortus. The biggest economic losses due to this disease are mortality, decreased production, stunted growth, and low body weight. The existence of resistance to synthetic anthelmintics causes the development of research on alternative treatments to herbal anthelmintics to be a strategic step. This study aims to analyze the effect of Areca catechu on Haemonchus contortus in vitro so that the Lethal Concentration 50 (LC 50) can be determined. This study was divided into 9 groups. Group I was treated with 2.5% A. catechu crude aqueous extract; group II was treated with 5% of A. catechu crude aqueous extract; group III was treated with 7.5% A. catechu crude aqueous extract; group IV was treated with 10% of A. catechu crude aqueous extract; group V was treated with 12.5% A. catechu crude aqueous extract; group VI was treated with 15% A. catechu crude aqueous extract; Group VII was treated with 17.5% A. catechu crude aqueous extract, group VIII was a negative control (0.9% NaCl) and group IX was a positive control (Albendazole). The mortality of H. contortus was recorded every hour until the worm mortality was 100%. The results were then analyzed using the Reed and Muench method. Based on the in vitro test of Areca catechu crude aqueous extract against H.contortus it can cause mortality in worms with an Lethal Concentration 50 (LC 50) calculation result of 11.11%.

Keywords

anthelmintic ;Areca cathecu;Haemonchus contortus; in vitro;LC50

Full Text:

PDF

References

Akkari, H., M.A. Darghout, and H.B. Salem. (2008). Preliminary investigations of the antinematode activity of Acacia cyanophylla Lindl.: Excretion gastrointestinal nematode eggs in lambs browsing A. Cyanophulla with and without PEG or grazing native grass. Journal of Small Ruminantia. Research. 74(b):78-83. Alawa, C.B.I., A.M. Adamu, J.O. Gefu, O.J. Ajanusi, P.A. Abdu, N.P. Chiezy, J.N. Alawa, and D.D. Bowman. (2003). In vitro screening of two Nigerian medicinal plants (Vernona amygdalina and Annona senegalensis) for anthelmintic activity. Journal of Veterinary Parasitology. 113:73-81. Alemu, Z., Y. Kechero, A. Kabede, and A. Muhammed. (2014). Comparison of the in vitro inhibitory effect of doses of tanin rich plant extract and ivermectin on egg hatchability, larvae development, and adult Mortality of Haemonchus contortus. Acta Parasitology. 59:513-519. Athanasiadou, S., Kyriazakis, I., Jackson, F. and Coop, R.L. (2001). Direct anthelmintic effects of condensed tannins towards different gas-trointestinal nematodes of sheep: in vitro and in vivo studies. Veterinary. Parasitology. 99, 205–219. Dalimartha, S. (2009). Atlas Tumbuhan Obat Indonesia. Jakarta. Penebar Swadaya Daryatmo, J., Hartadi, H., Orskov, E.R., Adiwimarta, K. And Nurcahyo, W. (2010). In vitro screening of various forages for anthelmintic activity on Haemonchus contortus eggs. Advances in Animal Biosciences. 1 : 113-113. Kuchai, J.A., F. Ahmad, M.Z., Chisty, Tak, J. Achmad, S. Ahmad and M. Rassol. (2012). A study on morpology and morphometry of Haemonchus contortus. Pakistan Journal of Zoology. 44(6):1737-1741. Menzies, P. (2006). Heaven on Earth for Sheep Parasites. Ontario Sheep Flock Health and management. http://www.ontariosheep.org/LinkClick.aspx?fileticket=ofhsMvSgsmo% 3D&tabid= 95 Min, B.R. and S.P. Hart. (2003). Tanins for supression of internal parasites. Journal of Animal Science. 81:E102-E109 Mortensen, L.L., L.H.Wiliamson, T.H. Terril, R. Kircher, M. Larsen, and R.M. Kaplan. (2003). Evaluation of prevalence and clinical implications of anthelmintic resistance in gastrointestinal nematodes of goats. Journal of the American Veterinary Medical Association. 23:495-500. Mubarokah, W.W., Nurcahyo, W., Prastowo, J. and Kurniasih, K. (2018). Daya Anthelmintik Infusa Biji Buah Pinang (Areca catechu) Terhadap Cacing Ascaridia galli Secara In Vitro. Jurnal Sain Veteriner. 37(1):252-257. Mubarokah, W.W., Nurcahyo, W., Prastowo, J. and Kurniasih, K. (2019).Pengaruh In Vitro Infusa Biji Buah Pinang (Areca catechu) terhadap Tingkat Kematian dan Morfometri Ascaridia galli Dewasa. Jurnal Sain Veteriner. 37(2):166-171. Mubarokah, W.W., Nurcahyo, W., Prastowo, J. and Kurniasih, K. (2019). The Population, Protein Profile and Ultrastructure of Ascaridia galli in Chicken Treated Using Areca catechu Crude Aqueous Extract. Journal of the Indonesian Tropical Animal Agriculture. 44(4):392-399. Reed, L.J., Muench, H. (1938). A Simple Method of Estimating Fifty Per Cent End points. The American Journal of Hygiene. 27(3): 493-497 Sandika, B., Raharjo & Ducha, N. (2012). Pengaruh Pemberian Air Rebusan Akar Delima (Punica granatum L) Terhadap Mortalitas Ascaris suum. Goesze. Secara In Vitro. LenteraBio, 1:81-86. Soulsby, E.J.L. (1986). Helminths Arthropods and Protozoa of Domesticated Animals. 7th ed. Bailiere Tindall, London.

DOI: https://doi.org/10.22146/jsv.70399