The Effect of Turmeric (Curcuma Domestica Val.) Extract on the Histopathological Lesions of Lung Induced by Cigarette Smokes in Wistar Rats

laelatul Rahmad(1*), Nida Ul Milah(2), Agapietta Kusumawardani(3), Novi Herliyani(4), Kurniati Sarwendah(5), Bambang Sutrisno(6), Hastari Wuryastuti(7), R Wasito(8)

(1) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(2) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(3) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(4) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(5) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(6) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(7) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(8) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(*) Corresponding Author


This present study is aimed to observe and determine the effects of turmeric (Curcuma domestica Val.) extract administration towards the cytotoxicity effect of cigarette smokes in the lungs of Wistar rats. Twenty Wistar rats, male, 3 months old, were used and divided randomly into four groups, of five each. Group I is as a control (K I), Group II was given 9 cigarettes/day without turmeric extract administration (K II), Group III given
turmeric extract 1 ml 0.25%/day (K III) and Group IV was given 9 cigarettes/day and turmeric extract 0.25%/day (K IV) for 60 days. At the end of the present study, all rats were necropsied and organs, particularly the lungs were collected, processed histopatologically and stained routenely with hematoxilyn eosin. The results indicated that the lungs had macrophages infiltration in the septal alveoli and hyperplasia of bronchial associated lymphoid
tissue (K II). Whereas, the lungs in the rats in Groups K I, K III and K IV were still within the normal limits. It is concluded, that turmeric extract may have a potential anti
pulmonary cytotoxity effect due to cigarette smoke.


turmeric extract, cigarretes smokes, Wistar rats, macrophages infiltration, hyperplasia of bronchial associated lymphoid tissue

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Daftar Pustaka

Alwan, A. (2012) WHO Report on the global

tobacco epidemic, 2011: Progress continuesnearly

8 billion people are now covered by an

effective tobacco control measure.WHO Press,

Avenue Appia, 1211 Geneva 27,


Aoshiba, K. and Nagai, A. (2003) Oxidative stress,

cell death and other damage to alveolar

epithelial cells induced by cigarette smoke. J.

Tobacco Induced Dis. 1: 219-226.

Banks, W.J. (1993) Applied veterinary histology 3rd.

Mosby, Philadelphia, USA. Pp 392-394,397-

Bienenstock, J. and Robert, L.C. (2007) Bronchus

associated lymphoid tissues: Inductive and

Effector Tissues and Cells of the Mucosal

Immune System.University of Alabama,

Birmingham, USA. http// diakses

pada tanggal 20 Mei, 2013.

Barnes, P.J and Manuel, G.S. (2004). Characteristic

of T lymphocytes in chronic obstructive

pulmonary disease. PloS Med. 1: 25-27.

Cunningham, J.G. (2002) Textbook of veterinary

physiology3rd ed. W.B. Saunders Company,

John Kennedy Boulevard #1800,

Philadelphia, USA.

Geiss, O. and Dimitrios, K. (2007) Tobacco,

cigarettes and cigarette smoke: An

overview.Institute for Health and Consumer

Protection, Italia.

diakses pada

tanggal 15 April, 2012.

Kumar, V., Abul, K.A., and Nelson, F. (2005)

Robbins and cotran pathologic basis of disease

th. Elsevier Saunders, The Curtis Center 170 S

Independence Mall W 300E, Philadelphia,


Laniado, R.L. (2009) Smoking and chronic

obstructive pulmonary disease (COPD),

parallel epidemics of the 21st century. Int. J.

Environ. Res. Public Health 6: 209-224.

Martey, C A., C. J. Baglole, T. A. Gasiewicz, P. J.

Sime and R. P. Phipps. (2005) The aryl

hydrocarbon receptor is a regulator of cigarette

smoke induction of the cyclooxygenase and

prostaglandin pathways in human lung

fibroblasts. J Phy. Lung Cell Mol. Phy. 289:

L391– L399.

Greaves, P. (2000) histopathology of preclinical

toxicity studies: Respiratory tract. 2nd ed. MRC

Toxicology Unit University of Leicester, UK.

http// diakses pada tanggal 20 Mei,

H i g d o n , J . ( 2 0 0 5 ) C u r c u m i n ,

Mazumder, R., Bhattaraya, S., Jha, S., Mazumder,

A., Kumari, J., and Jha, S. (2007) Studies on

pharmacognostical features of Curcuma

domestica Val. J. Ancient Scie. Life 26.4: 37-41.


phytochemical/ curcumin

Petrescu, F., V. Biciusca, Octavia, I.P., and C.M.,

Fortofoiu (2013) Cellular basis of bronchial

inflammation in chronic obstructive pulmonary

disease(COPD). J. Cur. Health Scie. 39: 61-67.

Rahman, I. (2002) Oxidative stress, chromatin

remodeling and gene transcription in

inflammation and chronic lung diseases. J.

Bioch.Mol. Biol. 36: 95-109.

Renne, R.A., D.L., Dungworth, C.M., Keenan, K.T.,

Morgan, F.F., Hahn, and L.W., Schwartz(2003)

Non-proliverative lesions of the respiraory

tract in rats. Guides Tox. Path.: 1-26.

Rahman, I. (2003) Oxidative stress, chromatin

remodeling and gene transcription in

inflammation and chronic lung diseases. Rev. J.

Bioch. Mol. Biol. 1: 95-109.

Reimondos, A., Utomo, I.D., McDonald, P., Hull, P.,

Suparno, H.and Utomo, A. (2012) The 2010

greater Jakarta transition to adulthood survey

policy background No. 2 smoking and young

adults in Indonesia diakses pada tanggal 18

November, 2012.

Samuelson, D.A. (2007) Textbook of veterinary

histology 2nd edition. Saunders Elsevier Inc.,

Westline Industrial Drive St. Louis,

Missouri, USA.

Shishodia, S., Tulika, S. and Madan, M.C. (2007)

Modulation of transcription factor by

curcumin. Adv. Exp. Med. Bio. 595: 127-148.

Tsai, S., Huang, S., Chyau, C., Tsai, C., Weng, C.,

and Mau, J. (2011) Composition and

antioxidant properties of essential oils from

Curcuma rhizome. Asian J. Arts Scie. 2: 57-66.

Thorley, A.J. and Teresa, D.T. (2007) Pulmonary

epithelium, cigarette smoke,and chronic

obstructive pulmonary disease. Int. J. COPD 2:


Tucker, M.J. (2003) Disease of Wistar. Taylor and

Francis Inc. 1900 Frost Road, Suite 101,

Bristol, Philadelphia, USA.

Venkatesan, N., Durairaj, P. and Mary, B. (2007)

Protection from acute and chronic lung disease

by curcumin in: the molecular targets and

therapeutic uses of curcumin in health and

disease. Adv. Exp. Med. Biol. 595:379-405.

Yoshida, T. and Rubin, M.T. (2007) Pathobiology of

cigarette smoke-induced chronic obstructive

pulmonary disease. Physiol. Rev.87:



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