* Corresponding Author
RAPID CONFIRMATION OF RABIES ANTIGEN IN FORMALIN-FIXED PARAFIN-EMBEDDED TISSUE BY IMMUNOPATHOLOGIC DIAGNOSIS
https://doi.org/10.22146/jsv.8625 R. Wasito(1*), Hastari Wuryastuti(2)
(1) Department of Pathology Faculty of Veterinary Medicine, Gadjah Mada University Yogyakarta
(2) Veterinary Internal Medicine, Faculty of Medicine, Gadjah Mada University, Yogyakarta
(*) Corresponding Author
Abstract
Skunks experimentally infected with street virus rabies
intramuscularly were used to demonstrate rabies viral antigen in
paraffin-embedded tissue by immunopathology diagnosis approach, the
avidin biotin complex (ABC) method. The use of ABC method provides a
simple and sensitive method to localize rabies virus antigen in formalin-
fixed tissues. The results of the present study also demonstrated that a
procedures using formalin-fixed parafin-embeded tissue and stained
immunopathologicalH by the ABC method was an excellent method for
both preservation of morfological details of ceils/tissues and demonstration
of rabies virus antigen.
Keywords
DOI: https://doi.org/10.22146/jsv.8625
Article Metrics
Abstract views : 1811
Refbacks
- There are currently no refbacks.
Copyright (c) 2015 Jurnal Sain Veteriner
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Sain Veteriner Indexed by
Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).
Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Jl. Fauna No.2, Karangmalang, Yogyakarta
Phone: 0274-560862
Fax: 0274-560861
Email: jsv_fkh@ugm.ac.id
HP. 0895363078367
Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.