Komparasi Metode coa Polymorphism dan coa Typing pada Bakteri Staphylococcus aureus yang Diisolasi dari Sumber Berbeda

Fatkhanuddin Aziz; Fauziah Fitriana; Dian Ritma Setyorini; Nur Ika Prihanani; Shafira Amalia Putri; Tifa Restyka Maulina; Vira Kartika Dewi; Morsid Andityas; Fajar Budi Lestari; Nurulia Hidayah; Risa Ummami; Achmad Fauzi

doi:10.22146/jsv.89815

Komparasi Metode coa Polymorphism dan coa Typing pada Bakteri Staphylococcus aureus yang Diisolasi dari Sumber Berbeda

https://doi.org/10.22146/jsv.89815

Fatkhanuddin Aziz^(1*), Fauziah Fitriana⁽²⁾, Dian Ritma Setyorini⁽³⁾, Nur Ika Prihanani⁽⁴⁾, Shafira Amalia Putri⁽⁵⁾, Tifa Restyka Maulina⁽⁶⁾, Vira Kartika Dewi⁽⁷⁾, Morsid Andityas⁽⁸⁾, Fajar Budi Lestari⁽⁹⁾, Nurulia Hidayah⁽¹⁰⁾, Risa Ummami⁽¹¹⁾, Achmad Fauzi⁽¹²⁾

(1) Department of Bioresources Technology and Veterinary, Vocational College, University of Gadjah Mada, Yogyakarta, Indonesia
(2) Department of Bioresources Technology and Veterinary, Vocational College, University of Gadjah Mada, Yogyakarta, Indonesia
(3) Department of Bioresources Technology and Veterinary, Vocational College, University of Gadjah Mada, Yogyakarta, Indonesia
(4) Department of Bioresources Technology and Veterinary, Vocational College, University of Gadjah Mada, Yogyakarta, Indonesia
(5) Department of Bioresources Technology and Veterinary, Vocational College, University of Gadjah Mada, Yogyakarta, Indonesia
(6) Department of Bioresources Technology and Veterinary, Vocational College, University of Gadjah Mada, Yogyakarta, Indonesia
(7) Department of Bioresources Technology and Veterinary, Vocational College, University of Gadjah Mada, Yogyakarta, Indonesia
(8) Department of Bioresources Technology and Veterinary, Vocational College, University of Gadjah Mada, Yogyakarta, Indonesia
(9) Department of Bioresources Technology and Veterinary, Vocational College, University of Gadjah Mada, Yogyakarta, Indonesia
(10) Department of Bioresources Technology and Veterinary, Vocational College, University of Gadjah Mada, Yogyakarta, Indonesia
(11) Department of Bioresources Technology and Veterinary, Vocational College, University of Gadjah Mada, Yogyakarta, Indonesia
(12) Department of Bioresources Technology and Veterinary, Vocational College, University of Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract

Staphylococcus aureus (S. aureus) merupakan golongan bakteri koagulase positif yang menyebabkan berbagai macam penyakit klinis pada manusia dan hewan. Determinasi pola gen coa merupakan salah satu metode yang sering digunakan dalam lingkup studi epidemiologi S. aureus. Penelitian ini dilakukan untuk membandingkan metode coa polymorphism dan coa typing pada isolat S. aureus yang diisolasi dari sumber berbeda. Tujuh belas koleksi isolat S. aureus asal susu pasteurisasi, susu mastitis kambing peranakan etawa, dan daging ayam segar ditumbuhkan dari stok gliserol -80^oC, kemudian dilakukan ekstraksi DNA dan amplifikasi gen penyandi koagulase (coa) dengan teknik PCR menggunakan primer untuk coa polymorphism dan coa typing. Diketahui, level diskriminasi coa typing lebih variatif dibandingkan dengan coa polymorphism pada 17 isolat yang diuji. Metode coa typing menunjukkan 7 tipe berbeda, sedangkan coa polymorphism hanya 3. Empat dari 17 isolat tidak dapat ditentukan coa typing, menunjukkan potensi pengembangan tipe baru coa typing untuk isolat-isolat asal Indonesia, untuk kepentingan studi epidemiologi.

Keywords

; Isolat; S. aureus; Tipe

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References

Ariyanti, D., Salasia, S. I. O., & Tato, S. (2011). Characterization of Haemolysin of Staphylococcus aureus Isolated from Food of Animal Origin. Indonesian Journal of Biotech. 16(1): 32-37. Aziz, F., Lestari, F. B., Nuraida, S., Purwati E., & Salasia, S. I. O. (2020). Deteksi Staphylococcus aureus dan Staphylococcus sp. Secara Langsung dari Susu Segar Kambing Peranakan Etawa dengan Teknik PCR. Jurnal Sain Veteriner. 38(2): 168–174. Bruce, S.A., Smith, J. T., Mydosh, J. L., Ball, J., Needle, D. B., Gibson, R., & Andam, C. P., (2022). Shared Antibiotic Resistance and Virulence Genes in Staphylococcus aureus from Diverse Animal Hosts. Scientific Reports, 12(1): 4413. Byrd, A. L., Deming, C., Cassidy, S. K., Harrison, O. J., Ng, W. I., Conlan, S., NISC Comparative Sequencing Program, Belkaid, Y., Segre, J. A., & Kong, H. H. (2017). Staphylococcus aureus and Staphylococcus epidermidis Strain Diversity Underlying Pediatric Atopic Dermatitis. Science translational medicine. 9(397): 4651. Chadi, Z. D., Dib, L., Zeroual, F., & Benakhla, A. (2022). Usefulness of molecular typing methods for epidemiological and evolutionary studies of Staphylococcus aureus isolated from bovine intramammary infections. Saudi Journal of Biological Sciences. 29(8): 103338. Cheung, G. Y., Bae, J. S., & Otto, M. (2021). Pathogenicity and Virulence of Staphylococcus aureus. Virulence. 12(1):547-569. Cho, Y. S., Lee, M. K., & Hwang, S. H. (2019). Toxin gene profiles, genetic diversity, antimicrobial resistance, and coagulase type of Staphylococcus aureus from cream‐filled bakery products. Food Science & Nutrition, 7(5), 1727-1734. Denayer, S., Delbrassinne, L., Nia, Y., & Botteldoorn, N. (2017). Food-borne Outbreak Investigation and Molecular Typing: High Diversity of Staphylococcus aureus Strains and Importance of Toxin Detection. Toxins. 9(12): 407. Effendi, M. H., Hisyam, M. A. M., Hastutiek, P., & Tyasningsih, W. (2019). Detection of Coagulase Gene in Staphylococcus aureus from Several Dairy farms in East Java, Indonesia, by Polymerase Chain Reaction. Veterinary World. 12(1): 68. Fitriana, F. (2022). Prevalensi Staphylococcus aureus Asal Susu Sapi Pasteurisasi yang Diperjualbelikan di Empat Kabupaten/Kota Daerah Istimewa Yogyakarta. Proyek Akhir. Program Studi Teknologi Veteriner Sekolah Vokasi Universitas Gadjah Mada. Yogyakarta. Fitriana, F., Resita, R., Disastra, Y., Alfatik, G.H., Artdita, C.A., Haryanto, A. & Aziz, F. (2022). Komparasi Lima Jenis Primer Polymerase Chain Reaction Untuk Mengidentifikasi Kelamin Burung Famili Columbidae Yang Akurat. Jurnal Sain Veteriner. 40(2): 205-220. Green, M.R. & Sambrook, J. (2019). Polymerase Chain Reaction. Cold Spring Harbor Protocols. 2019(6): 436-456. Hashemizadeh, Z., Dehkordi, R. S., Bazargani, A., Javadi, K., Hosseini-Nave, H., & Hadadi, M. (2022). Evaluation of aminoglycoside modifying enzymes, SCCmec, coagulase gene and PCR-RFLP coagulase gene typing of Staphylococcus aureus isolates from hospitals in Shiraz, southwest of Iran. Heliyon, 8(8). Hema, P., Appalaraju, B., & Someshwaran, R. (2022). Molecular Typing of Methicillin Resistant Staphylococcus aureus Using coa Gene Polymerase Chain Reaction-Restriction Fragment Length Polymorphism: A Cross-sectional Study. Journal of Clinical & Diagnostic Research. 16(11): 1-7. Hookey, J. V., Richardson, J. F., & Cookson, B. D. (1998). Molecular typing of Staphylococcus aureus Based on PCR Restriction Fragment Length Polymorphism and DNA Sequence Analysis of the Coagulase Gene. Journal of clinical microbiology. 36(4): 1083-1089. Kitagawa, H., Ohge, H., Hisatsune, J., Kajihara, T., Katayama, K., Takahashi, S. & Sugai, M. (2019). Prosthetic valve endocarditis caused by ST8 SCCmecIVl type community-associated methicillin-resistant Staphylococcus aureus. Internal Medicine, 58(5), 743-747. Le Loir, Y., Baron, F., & Gautier, M., (2003). Staphylococcus aureus and Food Poisoning. Genetics and molecular research: GMR. 2(1):63-76. Locatelli, C., Gattolin, S., Monistero, V., Castiglioni, B., Moroni, P., Addis, M. F., & Cremonesi, P. (2023). Staphylococcus aureus coa Gene Sequence Analysis Can Prevent Misidentification of Coagulase-Negative Strains and Contribute to Their Control in Dairy Cow Herds. Frontiers in Microbiology. 14:1120305. Martínez-Seijas, C., Mascarós, P., Lizana, V., Martí-Marco, A., Arnau-Bonachera, A., Chillida-Martínez, E. & Corpa, J. M. (2023). Genomic Characterization of Staphylococcus aureus in Wildlife. Animals, 13(6), 1064. Maulina, T. R. (2022). Metode Identifikasi Staphylococcus aureus pada Susu Kambing Mastitis di Kecamatan Samigaluh, Kulonprogo, Yogyakarta. Proyek Akhir. Program Studi Teknologi Veteriner Sekolah Vokasi Universitas Gadjah Mada. Yogyakarta. Miyake, R., Iwamoto, K., Sakai, N., Matsunae, K., Aziz, F., Sugai, M., Takahagi, S., Tanaka, A. & Hide, M. (2022). Uptake of Staphylococcus aureus by Keratinocytes is Reduced by Interferon–Fibronectin Pathway and Filaggrin Expression. The Journal of dermatology. 49(11): 1148-1157. Miyazawa, R., Shimoda, S., Matsuda, K., Tobe, R., Ando, T., & Yoneyama, H. (2022). Characterization of Staphylococcus aureus Isolates from Bovine Mastitis and Bulk Tank Milk: First Isolation of Methicillin-Susceptible Staphylococcus aureus in Japan. Microorganisms. 10(11): 2117. Momtaz, H., Tajbakhsh, E., Rahimi, E., & Momeni, M. (2011). Coagulase Gene Polymorphism of Staphylococcus aureus Isolated from Clinical and Sub-Clinical Bovine Mastitis in Isfahan and Chaharmahal va Bakhtiari Provinces of Iran. Comparative clinical pathology. 20: 519-522. Murugesan, A. C., Ramachandran, M., Varughese, H. S., & Kumaragurubaran, K. (2023). Staphylococcus coagulans Possesses Many Virulence Factors of Staph. aureus and Staph. pseudintermedius. Journal of Applied Microbiology. 134(1). Nadiya, S., Kolla, H. B., & Reddy, P. N., (2023). Optimization and Evaluation of a Multiplex PCR Assay for Detection of Staphylococcus aureus and Its Major Virulence Genes for Assessing Food Safety. Brazilian Journal of Microbiology. 54(1): 311-321. Neelam, Jain, V. K., Singh, M., Joshi, V. G., Chhabra, R., Singh, K. & Rana, Y. S. (2022). Virulence and Antimicrobial Resistance gene profiles of Staphylococcus aureus associated with clinical mastitis in cattle. Plos one. 17(5). Putri, S. A. (2023). Isolasi dan Identifikasi Staphylococcus sp. dan Staphylococcus aureus dari Sampel Daging Ayam yang Dijual di Sepuluh Pasar Wilayah Kota Yogyakarta. Proyek Akhir. Program Studi Teknologi Veteriner Sekolah Vokasi Universitas Gadjah Mada. Yogyakarta. Ren, Q., Liao, G., Wu, Z., Lv, J., & Chen, W. (2020). Prevalence and Characterization of Staphylococcus aureus Isolates from Subclinical Bovine Mastitis in Southern Xinjiang, China. Journal of dairy science. 103(4): 3368-3380. Sakai, F., Takemoto, A., Watanabe, S., Aoyama, K., Ohkubo, T., Yanahira, S., Igarashi, H., Kozaki, S., Hiramatsu, K. & Ito, T. (2008). Multiplex PCRs for Assignment of Staphylocoagulase Types and Subtypes of Type VI Staphylocoagulase. Journal of microbiological methods. 75(2): 312-317. Sato'o, Y., Omoe, K., Naito, I., Ono, H. K., Nakane, A., Sugai, M., Yamagishi, N., & Hu, D.L., (2014). Molecular Epidemiology and Identification of a Staphylococcus aureus Clone Causing Food Poisoning Outbreaks in Japan. Journal of clinical microbiology. 52(7): 2637-2640. Setyorini, D. R. (2023). Studi Pendahuluan Deteksi Staphylococcus sp. dan Staphylococcus aureus pada Daging Ayam Segar dari Pasar Tradisional. Proyek Akhir. Program Studi Teknologi Veteriner Sekolah Vokasi Universitas Gadjah Mada. Yogyakarta. Suwito, W., Nugroho, W. S., Adji, R. S., Andriani, A., Kusumaningtyas, E., & Martini, T. (2022). Phenotypic Characteristic of Staphylococcus aureus from Subclinical Mastitis in Etawah-crossbreed Goats in Yogyakarta, Indonesia. Veterinary World. 15(11). Thomas, S., Liu, W., Arora, S., Ganesh, V., Ko, Y.P., & Höök, M. (2019). The Complex Fibrinogen Interactions of the Staphylococcus aureus Coagulases. Frontiers in Cellular and Infection Microbiology. 9:106. Zhang, J., Wang, J., Jin, J., Li, X., Zhang, H., Shi, X., & Zhao, C. (2022). Prevalence, Antibiotic Resistance, and Enterotoxin Genes of Staphylococcus aureus Isolated from Milk and Dairy Products Worldwide: A systematic review and Meta-Analysis. Food Research International. 162:111969. Zhang, Z., Chen, Y., Li, X., Wang, X. and Li, H., 2022. Detection of Antibiotic Resistance, Virulence Gene, and Drug Resistance Gene of Staphylococcus aureus Isolate from Bovine Mastitis. Microbiology Spectrum. 10(4):00471-22.

DOI: https://doi.org/10.22146/jsv.89815