Pengaruh Paparan Sediaan Nanopartikel Ekstrak Etanol  Daun Mimba terhadap Struktur dan Fungsi Ren  Tikus Sprague Dawley Jantan

Ulfah Nuraini

doi:10.22146/jsv.99339

Pengaruh Paparan Sediaan Nanopartikel Ekstrak Etanol Daun Mimba terhadap Struktur dan Fungsi Ren Tikus Sprague Dawley Jantan

https://doi.org/10.22146/jsv.99339

Ulfah Nuraini^(1*)

(1) Diponegoro University
(*) Corresponding Author

Abstract

Technological advances in developing herbal plants as medicine continue to increase in Indonesia in finding herbal ingredients as medicine because they are considered safer, more practical and cheaper by the community compared to using synthetic drugs and easy to find the desired herbal ingredients. Neem plants (Azadirachta indica A.Juss) are herbal plants that have high antioxidant content and have the potential as medicine. The obstacle in oral administration is the low bioavailability of drugs and the distribution of active compounds in herbal plants. One way to overcome this problem is by packaging herbal plant extracts into smaller particle forms. This study used 32 rats aged 2 months which were divided into 4 treatments, each treatment had 8 replications, namely K-, K+, P1 and P2. The results showed that the administration of nanoparticle-chitosan preparations of ethanol extract of neem leaves 1; 0.5 and 1; 1 had no significant effect on glomerular diameter, Bowman's capsule diameter, kidney weight, creatinine levels and MDA levels p> 0.05 it can be concluded that the administration of nanoparticle-chitosan preparations of ethanol extract of neem leaves has no significant effect on the kidneys so that it can deliver the Bioactive compounds of neem leaves to the target organ, namely the kidneys and can minimize histological damage and kidney function of male white rats with the presence of antioxidant compounds in neem leaves.Technological advances in developing herbal plants as medicine continue to increase in Indonesia in finding herbal ingredients as medicine because they are considered safer, more practical and cheaper by the community compared to using synthetic drugs and easy to find the desired herbal ingredients. Neem plants (Azadirachta indica A.Juss) are herbal plants that have high antioxidant content and have the potential as medicine. The obstacle in oral administration is the low bioavailability of drugs and the distribution of active compounds in herbal plants. One way to overcome this problem is by packaging herbal plant extracts into smaller particle forms. This study used 32 rats aged 2 months which were divided into 4 treatments, each treatment had 8 replications, namely K-, K+, P1 and P2. The results showed that the administration of nanoparticle-chitosan preparations of ethanol extract of neem leaves 1; 0.5 and 1; 1 had no significant effect on glomerular diameter, Bowman's capsule diameter, kidney weight, creatinine levels and MDA levels p> 0.05 it can be concluded that the administration of nanoparticle-chitosan preparations of ethanol extract of neem leaves has no significant effect on the kidneys so that it can deliver the Bioactive compounds of neem leaves to the target organ, namely the kidneys and can minimize histological damage and kidney function of male white rats with the presence of antioxidant compounds in neem leaves.

Keywords

antioxidant; azadirachta indica; kidney; glomerular, nanoparticle

References

Anggraeni, S., Setyaningrum, T. and Listiawan, M. Y. (2017). Perbedaan Kadar Malondialdehid (MDA) sebagai Petanda Stres Oksidatif pada Berbagai Derajat Akne Vulgaris. Jurnal Kesehatan Kulit Dan Kelamin, 29(1):1–8. Anindita, R. (2019). Uji Seduhan Daun Teh Hijau (Camelia sinensis L). Pada Ginjal Mencit yang Diberi Monosodium Glutamat (MSG). Borneo Journal of Phamascientech. 3 (2):189-200. Fahriansyah, Isdadiyanto, S dan Sitasiwi, A. J. (2022). Gambaran Histologi Ren Tikus Putih (Rattus norvegicus L.) Hiperglikemia Setelah Pemberian Ekstrak Etanol Daun Mimba (Azadirachta Indica A. Juss). Buletin Anatomi dan Fisiologi. 6(2): 193-202. Ivanovna. (2014). Analisis Kadar Kreatinin Serum Sebelum dan Setelah Terapi Tenovofir pada Penyandang HIV di RS Dr. M. Djamil Padang Periode 2012-2013. Jurnal Kesehatan Andalas. 3(2): 1-6. Jumiarni, W.O. (2017). Eksplorasi Jenis Dan Pemanfaatan Tumbuhan Obat Pada Masyarakat Suku Muna Di Permukiman Kota Wuna. Traditional Medicine Journal. 22 (1): 45-56. Jiang shi, G., Y. Li, Q. H. Cao, H. X. Wu, X. Y. Tang, X. H. Gao, J. Q. Yu, Z. Chen, and Y. Yang. (2019). In Vitro And In Vivo Evidence That Quercetin Protects Against Diabetes And Itscomplications: Asystematic Review Of The Literature. Biomedicine and Pharmacotheraphy Journal. 109: 1085- 1099. Mayori R, N Marusin, & D. H. Tjong. (2014). Pengaruh pemberian Rhodamin B terhadap struktur histologis ginjal mencit putih (Mus musculus L.). Jurnal Biologi Universitas Andalas, 2(1): 1-7. Nugroho, Kanti, R. F., Dondin, S., dan Huda S. D. (2018). Profil Tekanan Darah Normal Tikus Putih (Rattus norvegicus) Galur Wistar dan Sprague-Dawley. Jurnal Veteriner. 6(2): 32-37. Kafshgari, M.H., Khorram, M., Khodadoost, M., dan Khavari, S. (2015). Reinforcement of Chitosan Nanoparticles Obtained by an Ionic Cross-Linking Process. Iran Polymer Journal. 20(5): 445- 456. Kurniasari, D., dan Atun, S. (2017). Pembuatan Dan Karakterisasi Nanopartikel Ekstrak Etanol Temu Kunci (Boesenbergia pandurata) Pada Berbagai Variasi Komposisi Alginat. Jurnal Kimia Dasar. 6(1): 31–35. Martien, R., Adhyatmika., Irianto, I.D.K., Verda, F., dan Sari, P.S. (2014). Perkembangan Teknologi Nanopartikel Sebagai Sistem Pengantaran Obat. Jurnal Farmaseutik. 8(1): 133-144. Patilaya, P., Azisah, N., dan Nerdy. (2015). Comparison of Antimicrobial Activity of Red Betel (Piper crocatum ruiz and Pav) Leaves Nanoparticle and Power Etanolic Extract Against Methicillin Resistant Staphylococcus Aureus. International Journal of PharmTech Research. 8(4): 696-701. Puspitasari M. L, Wulansari T. V, Widyaningsih TD, Mahar J. (2016). Aktivitas Antioksidan Suplemen Herbal Daun Sirsak (Annona muricata L) dan Kulit Manggis (Garcinia mangostana L). Jurnal Pangan Dan Agroindustri. 4(1): 283–90. Septiva E. B., Sitasiwi, A. J., dan Isdadiyanto, S. (2019). Struktur Mikroanatomi Ginjal Mencit (Mus musculus L.) Betina Setelah Paparan Ekstrak Etanol Daun Mimba (Azadirachta indica A. Juss). Jurnal Pro-Life. 6(2): 180- 190. Situmorang, R. (2015). Pengaruh Ekstrak Etanol Daun Bangun bangun (Plectranthus amboinicus (Lour.) Spreng) Sebagai Preventif dan Kuratif Terhadap Efek Toksik Rhodamin B Pada Histopatologi Ginjal Tikus Putih (Rattus norvegicus). Jurnal Biosains. (1)3: 73-85. Supriyanto S, B Sinon, M Rivai, dan Yunianta. (2017). Uji fitokimia dan aktivitas antioksidan ekstrak daun mimba (Azaradiracta indica A. Juss). Prosiding SNATIF. 523- 529. Tana, S., Shivaluhung, M. Suprihatin, T. (2022). Gambaran Histologi Ren Tikus Putih (Rattus norvegicus L.) yang Diinduksi Insulin. Buletin Anatomi dan Fisiologi. 7(2): 127- 134.

DOI: https://doi.org/10.22146/jsv.99339