Dosis Efektif Anestesi Kombinasi Ketamine-xylazine pada Tikus Pediatri

Muhammad Luthfi Rahman; Titiek Sunartatie; Altaff Hendry; Teo Qin Yan; Dian Maulia Utami; Daniel Latief Andre; Win Satya Rudramurti; Diah Nugrahani Pristihadi

doi:10.22146/jsv.99829

Dosis Efektif Anestesi Kombinasi Ketamine-xylazine pada Tikus Pediatri

https://doi.org/10.22146/jsv.99829

Muhammad Luthfi Rahman⁽¹⁾, Titiek Sunartatie⁽²⁾, Altaff Hendry⁽³⁾, Teo Qin Yan⁽⁴⁾, Dian Maulia Utami⁽⁵⁾, Daniel Latief Andre⁽⁶⁾, Win Satya Rudramurti⁽⁷⁾, Diah Nugrahani Pristihadi^(8*)

(1) Program Studi Sarjana Kedokteran Hewan, Sekolah Kedokteran Hewan dan Biomedis, IPB University, Bogor, Jawa Barat, Indonesia
(2)
(3) Program Studi Sarjana Kedokteran Hewan, Sekolah Kedokteran Hewan dan Biomedis, IPB University, Bogor, Jawa Barat, Indonesia
(4) Program Studi Sarjana Kedokteran Hewan, Sekolah Kedokteran Hewan dan Biomedis, IPB University, Bogor, Jawa Barat, Indonesia
(5) Program Studi Sarjana Kedokteran Hewan, Sekolah Kedokteran Hewan dan Biomedis, IPB University, Bogor, Jawa Barat, Indonesia
(6) Program Studi Sarjana Kedokteran Hewan, Sekolah Kedokteran Hewan dan Biomedis, IPB University, Bogor, Jawa Barat, Indonesia
(7) Program Studi Sarjana Kedokteran Hewan, Sekolah Kedokteran Hewan dan Biomedis, IPB University, Bogor, Jawa Barat, Indonesia
(8) Fakultas Kedokteran Hewan IPB University, Bogor, Jawa Barat, Indonesia
(*) Corresponding Author

Abstract

Anestesi efektif mengurangi stres dan rasa sakit pada tikus dengan menghilangkan kesadaran. Kombinasi yang umum digunakan dalam penelitian adalah Ketamine dan Xylazine. Kombinasi ini telah banyak digunakan pada tikus dewasa dengan berbagai dosis, tetapi belum ada acuan dosis tepat untuk tikus pediatri. Penelitian ini bertujuan mendapatkan dosis anestesi dari kombinasi Ketamine-Xylazine yang efektif dan aman untuk tikus pediatri. Penelitian menggunakan 25 ekor tikus berumur 3 minggu yang dibagi menjadi 5 kelompok perlakuan, yaitu kelompok negatif dan 4 perlakuan dosis Ketamine (25, 50, 75, dan 100 mg/kg BB) dikombinasikan dengan Xylazine 4 mg/kg BB. Hasil penelitian menunjukkan efek anestesi tikus pediatri baru mulai muncul setelah pemberian dosis Ketamine 50 mg/Kg BB. Kondisi fisiologis (frekuensi jantung, frekuensi napas, dan suhu tubuh) mengalami penurunan pada 10 menit setelah injeksi anestesi dan seiring waktu berangsur kembali ke normal. Pada dosis yang tinggi (100 mg/kg BB), terjadi kematian pasca injeksi dengan tingkat kematian 40%. Dosis anestesi Ketamine-Xylazine yang disarankan adalah 50–75/4 mg/kg BB.

Keywords

dosis; ketamine; pediatri; tikus; xylazine

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References

Alemán-Laporte, J., Bandini, L.A., Garcia-Gomes, M.S., Zanatto, D.A., Fantoni, D.T., Amador Pereira, M.A., Navaz-Suárez, P.E., Kirsten, T.B., Jimenez, R.R., Alvarado, G., Mori, C. C. (2020). Combination of ketamine and xylazine with opioids and acepromazine in rats: physiological changes and their analgesic effect analysed by ultrasonic vocalization. Laboratory animals, 54(2), 171-182. Aripin, D.N. (2015). Efektivitas anestetikum kombinasi medetomidin dengan Ketamine pada kucing lokal Indonesia. [Skripsi]. Bogor (ID): Institut Pertanian Bogor. Baumgartner, C., Bollerhey, M., Ebner, J., Laacke-Singer, L., Schuster, T., Erhardt, W. (2010). Effects of Ketamine-Xylazine intravenous bolus injection on cardiovascular function in rabbits. CJVR. 74 (3): 200–208. Bryant, S. (2010). Anesthesia for veterinary technicians. Wiley-Blackwell, Iowa. Camp, C.R., Yuan, H. (2020). GRIN2D/GluN2D NMDA receptor: Unique features and its contribution to pediatric developmental and epileptic encephalopathy. Eur J Paediatr Neurol. 24: 89–99. Cascella, M., Bimonte, S., Di Napoli, R. (2020). Delayed emergence from anesthesia: What we know and how we act. Local Reg Anesth. pp. 195–206. Cornick, J.L. (1994). Veterinary anesthesia. Di dalam: Dennis MM, editor. Clinical Textbook for Veterinary Technicians. Ed ke-3. WB Saunders, Philadelphia. Danneman, P.J., Mandrell, T.D. (1997). Evaluation of five agents/methods for anesthesia of neonatal rats. Lab Anim Sci. 47: 386–395. Djuric, M., Kostic, S., Nikolic Turnic, T., Stankovic, S., Skrbic, R., Djuric, D. M., Zivkovic, V., Jakovljevic V., Stevanovic, P. (2020). The comparison of the effects of ketamine and etomidate on cardiodynamics, biochemical and oxidative stress parameters in Wistar male rats. Molecular and Cellular Biochemistry, 474, 125-134. Gagarinskiy, E. L., Averin, A. S., Uteshev, V. K., Sherbakov, P. V., Telpuhov, V. I., Shvirst, N. E., Karpova, Y. A., Gurin, A. E., Varlachev, A. V., Kovtun, A. L., Fesenko, E. E. (2022). Time Limiting Boundaries of Reversible Clinical Death in Rats Subjected to Ultra-Deep Hypothermia. Annals of cardiac anaesthesia, 25(1), 41–47. https://doi.org/10.4103/aca.ACA_189_20. Geier, M.R., Geier, D.A. (2020). Respiratory conditions in coronavirus disease 2019 (COVID-19): Important considerations regarding novel treatment strategies to reduce mortality. Med Hypotheses.140: 109760. Ghasemi, A., Jeddi, S., Kashfi, K. (2021). The laboratory rat: Age and body weight matter. EXCLI journal. 20: 1431–1445. doi: 10.17179/excli2021-4072. Huang, M., Watso, J.C., Moralez, G., Cramer, M.N., Hendrix JM, Yoo JK, Crandall CG. (2020). Low‐dose Ketamine affects blood pressure, but not muscle sympathetic nerve activity, during progressive central hypovolemia without altering tolerance. Physiol J. 598 (24): 5661–5672. Hou, G., Zhang, Z.W. (2017). NMDA Receptors regulate the development of neuronal intrinsic excitability through cell-autonomous mechanisms. Front Cell Neurosci. 11: 353. Kamel, I., Ahmed, M.F, Sethi, A. (2022). Regional anesthesia for orthopedic procedures: What orthopedic surgeons need to know. World J Orthop. 13 (1): 11–35. doi: 10.5312/wjo.v13.i1.11. Katzung, B.G. (2007). Agents used in hyperlipidemia. In Basic and Clinical Pharmacology. Edisi ke-10. Mc Graw-Hill, New York. Lim, S. Y., Pettit, R. S. (2019). Pharmacokinetic considerations in pediatric pharmacotherapy. American Journal of Health-System Pharmacy, 76(19), 1472-1480. Mahmood, M.B. (2022). A comparison between Ketamine-Xylazine and Ketamine-midazolam or all of them to induce balance anesthesia in rabbits. Iraqi J Vet. 36 (2): 499–506. Megda, T.T., de Sousa, F.G., Miranda, F.G., Barreto MSO, Mattoso CRS, Beier SL. (2022). Echocardiographic assessment of felines sedated with dexmedetomidine or Xylazine associated with butorphanol. Res Soc Dev. 11 (12): e559111234902. Olcese, U., Lohuis, M.N.L., Pennartz, C.M.A. (2018). Sensory processing across conscious and nonconscious brain states: From single neurons to distributed networks for inferential representation. Front Syst Neurosci. 12: 49. doi: 10.3389/fnsys.2018.00049. Orhurhu, V.J., Vashisht, R., Claus, L.E., Cohen, S.P. (2023). Ketamine Toxicity. [Updated 2023 Jan 30]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK541087/. Riccardi, A., Guarino, M., Serra, S., Spampinato, M.D., Vanni, S., Shiffer, D., Voza, A., Fabbri, A., De Iaco, F. (2023). Narrative review: Low-dose Ketamine for pain management. J Clin Med. 12 (9): 3256. doi: 10.3390/jcm12093256. Rogers, K.L. (2020). Guidelines for anesthesia and analgesia in rats. Indiana University Bloomington. [diakses 2024 Juli 1]. https://lar.indiana.edu/policies-guidelines/Guidelines-for-Anesthesia-and-Analgesia-in-Rats-FINAL.docx Sabrina, S., Sabine, T., Brian, G., Julia, H. (2016). Comparison of physiological parameters and anesthesia specific observations during isoflurane, ketamine- xylazine or medetomidine-midazolam-fentanyl anesthesia in male guinea pigs. PLoS One. pp. 1–22. doi: 10.1371/journal.pone.0161258. Singer, D. (2021). Pediatric hypothermia: An ambiguous issue. Int J Environ Res Public Health. 18 (21): 11484. Sotoudeh, N., Namavar, M.R. (2022). Optimisation of Ketamine-Xylazine anaesthetic dose and its association with changes in the dendritic spine of CA1 hippocampus in the young and old male and female Wistar rats. Vet med sci. 8 (6): 2545–2552. doi: 10.1002/vms3.936. Suckow, M.A, Wilson RP, Hankenson FC, Foley PL. (2020). The Laboratory Rat. Edisi ke-3. Elsevier, London. Tsukamoto, A., Konishi, Y., Kawakami, T., Koibuchi, C., Sato, R., Kanai, E., Inomata, T. (2017). Pharmacological properties of various anesthetic protocols in 10-day-old neonatal rats. Exp Anim. 66 (4): 397–404. doi: 10.1538/expanim.17-0037.

DOI: https://doi.org/10.22146/jsv.99829