Isolation and Characterization of Lactic Acid Bacteria from Inasua

https://doi.org/10.22146/jtbb.16380

Ferymon Mahulette(1*), Nisa Rachmania Mubarik(2), Antonius Suwanto(3), Widanarni Widanarni(4)

(1) Postgraduate School of Bogor Agricultural University
(2) Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University
(3) Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University
(4) Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University
(*) Corresponding Author

Abstract


Inasua is a traditionally product of wet salt fish fermentation produced by Teon, Nila and Serua (TNS) Communities in Central Maluku, Indonesia. The community made this fermented fish to anticipate the lean time when fisherman could not go to sea.  The  fish that used as inasua raw material is demersal fishes that live around coral reefs, such as Samandar fish (Siganatus guttatus), Gala-gala fish (Lutjanus sp.) and Sikuda fish (Lethrinus ornatus). The objective of the research was to isolate and characterize of bacterial indigenous in  Inasua from three producers in Seram Island. The measurement of pH from inasua samples were 5.9, 5.0 and 5.8, respectively. The highest number of lactic acid bacteria was found from  Gala – gala inasua was 2,5x107 cfu/g sample. Isolation of all isolates bacteria from inasua showed that a total of 7 isolates of bacteria was obtained  from Samadar inasua, 9 isolates from  Gala-gala inasua, and 7 isolates from  Sikuda inasua.  From a total of 23 isolates, only 6 isolates had characteristic as lactic acid bacteria that were Gram  positive, negative catalase, and cocci shape. The microscopic characteristics  of the isolates are coccid in pairs or uniforms which combine to form tetrads. Carbohydrate utilization test  of selected isolate by using API 50 CHB kit indicated that 13 carbohydrates are fermented by these isolates  after incubation for 48 hours. The research  was concluded that the dominant bacteria in inasua sample  is  cocci-lactic acid bacteria.

Keywords : fermented fish, inasua, lactic acid bacteria, MRSA medium


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References

Atputharajah JD, Widanapathirana S, Samarajewa U. 1986. Microbiology and biochemistry of natural fermentation palm sap. Food Microbiol. 3:273-280.

Borse BB, Rao LJM, Ramalakshmi K, Raghavan B. 2007. Chemical composition of volatiles from coconut sap (neera) and effect of processing. Food Chem. 101:877–880.

Carr JF, Chill D, Maida N. 2002. The lactic acid bacteria: a literature survey. Crit Rev Microbiol. 28(4):281-370.

Fan L, Song J. 2013. Microbial pathogens and strategies for combating them: science, technology and education. Mendez-Vilas A, editor. Badajoz (ES): Formatex Research Center.

Holzapfel WH, Franz CMAP, Ludwig W, Back W, Dick LMT. 2006. The genera Pediococcus and Tetragenococcus. Procaryotes. 4:229-266.

Jay JM. 1996. Modern Food Microbiology. Edisi ke-4. New York (US): Chapman and Hall. Kimhamanon T. 1994. Microbial change during production of fermented fish (som-fag) [thesis]. Songkla (TH): Prince of Songkla University.

Kopermsub P, Yunchalard S. 2008. Safety control indices for plaa-som, a Thai fermented fish product. Afr J Microbio Res. 2:18-25.

Kopermsub P, Yunchalard S. 2010. Identification of lactic acid bacteria associated with the production of plaa-som, a traditional fermented fish product of Thailand. Int J Food Microbiol. 138:200-204.

Kusmarwati A, Indriati N, Hermana I. 2013. Production and chraracterization bacteriocin produced by lactic acid bacteria isolated from rusip. Squalen. 8(1):13-22.

Nara S, Ijong F, Suwetja IK, Onibala H. 2013. Ina sua, a fermented salted fish product from central Moluccas. Aquat Sci Manag. 1(2):160-164.

Nendissa SJ. 2013. Pengaruh penambahan Pediococcus acidilactici F11 sebagai kultur starter terhadap kualitas ikan asin (ina sua) bae (Lutjanus malabaricus). Ekosains. 2(1):39-46.

Njoki WJ, Boga HI, Kutima PM, Maina MJ, Kadere TT. 2015. Probiotic potential of lactic acid bacteria from coconut (Cocos nucifera) wine (mnazi) in Kenya. Int J Life Sci Res. 3(1):113-120.

Noonpakdee W, Jumriangrit P, Wittayakom K, Zendo J, Nakayama J, Sonomoto, K, Panyim S. 2009. Two-peptide bacteriocin from Lactobacillus plantarum PMU 33 strain isolated from som-fak, a Thai low salt fermentation fish product. Asia Pac J Mol Biol Biotechnol. 17(1):19-25.

Owens JD, Mendoza LS. 1985. Enzymically hydrolysed and bacterially fermented fishery product. J Food Technol. 20:273-295.

Paludan-Muller CP. 2001. The Microbiology of Low Salt Fermented Fish Product. Lyngby (DK): Danish Technical University. Paludan-Muller CP, Madsen M, Sophanodora P, Gram L, Moller PL. 2002. Fermentation and microflora of plaa-som, a Thai fermented fish product prepared with different salt concentration. Int J Food Microbiol. 73: 61-70.

Panda SH, Ray RC, El Sheikha AF, Montet D, Worawattanamateekul W, 2011. Fermented fish and fish product. Aquacul Microbiol Biotechnol. 2:132-172.

Pringsulaka O, Thongngam N, Suwannasai N, Atthakor W, Pothivejkul K, Rangsiruji A. 2012. Partial characterization of bacteriocins produced by lactic acid bacteria isolated from Thai fermented meat and fish product. Food Contr. 23:547-551.

Saisithi P. 1994. Fisheries Processing: Biotechnological Application. Martin AM, editor. London (GB): Chapman and Hall.

Saithong P, Panthavee W, Boonyaratanakornkit M, Sikkhamondhoi C. 2010. Use of starter culture of lactic acid bacteria in plaa-som, a Thai fermented fish. J Biosci Bioeng. 110(5):553-557.

Simpson WJ, Taguchi H. 1995. The genus Pediococcus, with notes on the genera Tetragenococcus and Aerococcus. Wood JB, Holzapfel WH, Editor. London (GB): Blackie Academic & Professional.

Walalohun M. 2014. Analisis kelimpahan bakteri Staphylococcus pada ikan inasua berdasarkan kadar garam dan lama fermentasi [skripsi]. Ambon (ID) : Universitas Pattimura.



DOI: https://doi.org/10.22146/jtbb.16380

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