* Corresponding Author
Ulva lactuca Linnaeus Potentially Promotes Reproductive Indices and Depressive-like Behavior of Hypertriglyceridemia Male Wistar Rats (Rattus norvegicus Berkenhout, 1769)
https://doi.org/10.22146/jtbb.57924 Anggoro Chandra Yulistiyanto(1), Mayumi Hersasanti(2), Rahadian Yudo Hartantyo(3), Laksmindra Fitria(4), Abdul Razaq Chasani(5), Mulyati Mulyati(6*)
(1) Faculty of Biology, Universitas Gadjah Mada
(2) Faculty of Biology, Universitas Gadjah Mada
(3) Faculty of Biology, Universitas Gadjah Mada
(4) Faculty of Biology, Universitas Gadjah Mada
(5) Faculty of Biology, Universitas Gadjah Mada
(6) Faculty of Biology, Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Excessive consumption of fatty foods can lead to hyperlipidemia, which is often coupled with hypertriglyceridemia (HTG), a condition where blood plasma triglyceride (TG) levels elevated beyond normal levels. This condition may disturb physiological functions of the body, such as reproductive functions, and other physiological imbalances leading to chronic stress and depression. Ulva lactuca is a potential natural treatment for HTG, as it contains various nutrients to aid physiological functions. This seaweed also has high levels of Cd, which can increase depression. Therefore, research on the potential benefits of U. lactuca should be followed by an investigation of its health risks. This research aimed to examine the effects of HTG and treatment with U. lactuca on reproduction and depressive-like behavior of male Wistar rats (Rattus norvegicus Berkenhout, 1769). The data collected in this research include body weight, serum TG concentration, gonadosomatic index (GSI), serum testosterone concentration using competitive ELISA, and depressive-like behaviors assessed using the Forced Swim Test (FST) and Open Field Test (OFT). Data were analyzed using One-Way ANOVA followed by DMRT, independent- and paired-samples t-test, and Kruskal-Wallis H test with a significance value of α=0.05. Body weight, serum TG and testosterone concentration, GSI, and depressive-like behaviors were increased by the HTG condition. Ulva lactuca at the1500 mg/kg BW/day did not significantly affect body weight, testosterone concentration, and depressive-like behaviors of HTG rats. Meanwhile, this treatment significantly increased the GSI and depressive-like behaviors of healthy rats. These results suggest that Ulva lactuca treatment not only enhances gonad growth and development but also increases depressive-like behaviors.
Keywords
Full Text:
PDFReferences
Alvarado, L.C., C. R. Valeggia, P. T. Ellison, C. L. Lewarch, and M. N. Muller, 2019, A Comparison of men’s Life History, Aging, and Testosterone Levels among Datoga Pastoralists, Hadza Foragers, and Qom Transitional Foragers, Adaptive Human Behavior and Physiology 5(3), 251-273.
André, V., C. Gau, A. Scheideler, J. A. Aguilar-Pimentel, O. V. Amarie, L. Becker, L. Garrett, W. Hans, S. M. Hölter, D. Janik, and K. Moreth, 2018, Laboratory mouse housing conditions can be improved using common environmental enrichment without compromising data, PLoS biology 16(4), 1-24.
Ashakiran and Deepth R, 2012, Fast Food and Their Impact on Health, JKIMSU 1(2), 1-9.
Aukett, M. A., Y. A. Parks, P. H. Scott, and B. A. Wharton, 1986, Treatment with iron increases weight gain and psychomotor development, Archives of disease in childhood, 61(9), 849-857.
Banks, W.A., S.A. Farr, T.S. Salameh, M.L. Niehoff, E.M. Rhea, J.E. Morley, A.J. Hanson, K.M. Hansen, and S. Craft, 2018, Triglycerides cross the blood-brain barrier and induce central leptin and insulin receptor resistance, International Journal of Obesity 42, 391-397.
Barber, B.J. and N.J. Blake, 2006, Reproductive physiology, In: Scallops: Biology, ecology, and aquaculture (S.E. Shumway and G.J. Parsons, Eds.) Second Ed, Elsevier Science Pub, Florida, pp. 357–416.
Berglund, L., J.D. Brunzell, A.C. Goldberg, I.J. Goldberg, F. Sacks, M.H. Murad, & A.F.H. Stalenhoef. 2012. Evaluation and Treatment of Hypertriglyceridemia: An Endocrine Society Clinical Practice Guideline. Journal of Clinical Endocrinology & Metabolism 97(9):2969-2989.
Blesso, C.N. and M.L. Fernandez, 2018, Dietary Cholesterol, Serum Lipids, and Heart Disease: Are Eggs Working for or Against You?, Nutrients 10(4), 1-12.
[BPOM] Badan Pengawas Obat dan Makanan. 2018. Peraturan Badan Pengawas Obat dan Makanan Nomor 5 Tahun 2018 tentang Batas Maksimum Cemaran Logam Berat dalam Pangan Olahan (BPOM Regulation Number 5 of 2018 on the Maximum Limit of Heavy Metal Contamination in Processed Foods). Jakarta: BPOM.
Carrier, N., S.K. Saland, F. Duclot, H. He, R. Mercer, and M. Kabbaj, 2018, The Anxiolytic and Antidepressant-like Effects of Testosterone and Estrogen in Gonadectomized Male Rats, Biological Physchiatry 78(4), 259-269.
Choleris, E., A.W. Thomas, M. Kavaliers, and F.S. Prato, 2001, A detailed ethological analysis of the mouse open field test: effects of diazepam, chlordiazepoxide and an extremely low frequency pulsed magnetic field, Neuroscience and Biobehavioral Reviews 25, 235-260.
Cryan, J.F. and A. Holmes, 2005, The ascent of mouse: advances in modelling human depression and anxiety, Nature reviews Drug discovery 4(9), 775-790.
De Keyser, C.E., F.V. De Lima, F.H. de Jong, A. Hofman, Y.B. de Rijke, A.G. Uitterlinden, L.E. Visser, and B.H. Stricker, 2015, Use of statins is associated with lower serum total and non-sex hormone-binding globulin-bound testosterone levels in male participants of the Rotterdam Study, European journal of endocrinology 173(2), 155-165.
Dewantari, N.M., 2013, Peranan Gizi dalam Kesehatan Reproduksi [The Role of Nutrition in Reproductive Health], Jurnal Skala Husada 10(2), 219-224.
DiaSys, 2015, Triglycerides FS, Holzheim: DiaSys Diagnostic Systems GmbH, pp. 1-2.
Ennaceur, A., 2014, Tests of unconditioned anxiety – Pitfalls and disappointments, Physiology & Behavior 135, 55-71.
Erniati, F.R. Zakaria, E. Prangdimurti, and D.B. Adawiyah, 2016, Potensi Rumput Laut: Kajian Komponen Bioaktif dan Pemanfaatannya sebagai pangan Fungsional [Potential of Seaweed: A Study of Bioactive Components and Its Use as Functional Food], Aquatic Sciences Journal 3(1), 12-17.
Freeman, D.A. and J.A. Ontko, 1992, Accumulation and mobilization of triglycerides and cholesteryl esters in Leydig tumor cells, J. Lipid Res 33, 1139-1146.
Frick M.H., O. Elo, K. Haapa, 1987, Helsinki Heart Study, primary-prevention trial with Gemfibrozil in middle-aged men with dyslipidemia. Safety of treatment; changes in risk factors; and incidence of coronary heart disease, The New England journal of medicine 317(20), 1237–1245.
Ge, T., J. Fan, W. Yang, R. Cui, and B. Li, 2018, Leptin in depression: a potential therapeutic target. Cell Death & Disease 9, 1096.
Hassan, S., S.A. El-Twab, M. Hetta, and B. Mahmoud, 2011, Improvement of lipid profile and antioxidant of hypercholesterolemic albino rats by polysaccharides extracted from the green alga Ulva lactuca Linnaeus, Saudi journal of biological sciences 18. 333-340.
Hu, J., Z. Zhang, W.J. Shen, and S. Azhar, 2010, Cellular cholesterol delivery, intracellular processing and utilization for biosynthesis of steroid hormones, Nutrition & metabolism 7(1), 1-25.
Jung, M.K. and E.G. Yoo, 2018, Hypertriglyceridemia in Obese Children and Adolescents, Journal of obesity & metabolic syndrome, 27(3), 143-149.
Kamel, M.M., A.H.A.E. Razek, K.A. Ahmed, and G.M. Kamel, 2011, Exposure of Adult Male Rats to Cadmium: Assessment of Sexual Behaviour, Fertility, Aggression as well as Anxiety like Behaviour with Special Reference to Biochemical and Pathological Alterations, Life Science Journal 8(2), 106-120.
Kidgell, J.T., M. Magnusson, R. de Nys, and C. R. Glasson, 2019, Ulvan: A systematic review of extraction, composition and function, Algal research 39 (2019), 1-2.
Lamtai, M., et al., 2018, Effect of Chronic Administration of Cadmium on Anxiety-Like, Depression-Like and Memory Deficits in Male and Female Rats: Possible Involvement of Oxidative Stress Mechanism, Journal of Behavioral and Brain Science 8, 240-268.
Lawson, E.A., K.K. Miller, J.I. Blum, E. Meenaghan, M. Misra, K.T. Eddy, D.B. Herzog, and A. Klibanski, 2012, Leptin Levels are Associated with Decreased Depressive Symptoms in Women Across the Weight Spectrum, Independent of Body Fat, Clinical Endocrinology 76(4), 520-525.
Lee, G., S. Lee, N. Ha, Y. Kho, K. Park, P. Kim, B. Ahn, S. Kim, and K. Choi, 2019, Effects of Gemfibrozil on sex hormones and reproduction related performances of Oryzias latipes following long-term (155 d) and short-term (21 d) exposure, Ecotoxicology and environmental safety 173, 174-181.
Lockman K.A., J.P. Baren, C.J. Pemberton, H. Baghdadi, K.E. Burgess, N. Plevris-Papaioannou, P. Lee, F. Howie, G. Beckett, A. Pryde, A.J. Jaap, P.C. Hayes, C. Filippi, and J.N. Plevris, 2012, Oxidative Stress rather than Triglyceride Accumulation is a Determinant of Mitochondrial Dysfunction in In Vitro Models of Hepatic Cellular Steatosis, Liver International 2012, 1-14.
Lu, X-Y., 2007, The leptin hypothesis of depression: a potential link between mood disorders and obesity, Current Opinion in Pharmacology 7(6), 648-652.
Magara, S., S. Holst, S. Lundberg, E. Roman, and M. Lindskog, 2015, Altered explorative strategies and reactive coping style in the FSL rat model of depression, Frontiers in Behavioral Neuroscience 9(89), 1-13.
Minguez-Alarcon L., J.E. Chavarro, J. Mendiola, M. Roca, C. Tanrikut, J. Vioque, N. Jorgensen, A.M. Torres-Cantero, 2017, Fatty Acid Intake in Relation to Reproductive Hormones and Testicular Volume among Young Health Men, Asian Jounal of Andrology 2017(19), 184-190.
Mulyati, A.C. Yulistiyanto, M. Hersasanti, and Z. Rais, 2019, Potensi NutriUlva sebagai Supplemen Hematologis [Potential of NutriUlva as a Hematological Supplement], Research Colaboration Lecturer and Student Universitas Gadjah Mada, pp. 1-18.
Nistiar, F., O. Racz, A. Lukacinova, B. Hubkova, J. Novakova, E. Lovasova, and E. Sedlakova, 2012, Age dependency on some physiological and biochemical parameters of male Wistar rats in controlled environment, Journal of Environmental Science and Health Part A 47(9), 1224-1233.
Nurhidayat L., F.N. Arviani, and B. Retnoaji, 2017, Indeks Gonadosomatik dan Struktur Histologis gonad Ikan Uceng (Nemacheilus fasciatus, Valenciennes in Cuvier and Valenciennes, 1846) [Gonadosomatic Index and Histological Structure of Uceng Fish (Namacheilus fasciatus, Valenciennes in Cuvier and Valenciennes, 1846) Gonads], Biosfera 34(2), 67-74.
Ofei, F., 2005, Obesity-a preventable disease, Ghana medical journal 39(3), 98-101.
Planchez, B., A. Surget, and C. Belzung, 2019, Animal models of major depression: drawbacks and challenges, Journal of Neural Transmission 126, 1383-1408.
Sathivel, A., H.R.B. Raghavendran, P. Srinivasan, and T. Devaki, 2008, Antiperoxidative and anti-hyperlipidemic nature of Ulva lactuca crude polysaccharide on D-Galactosamine induced hepatitis in rats, Food and Chemical Toxicology 46(10), 3262-3267.
Scinicariello, F. and M.C. Buser, 2015, Blood Cadmium and Depressive Symptoms in Young Adults (20-39 years), Psychological Medicine 45(4), 807-815.
Seibenhener, M.L. and M.C. Wooten, 2015, Use of the Open Field Maze to Measure Locomotor and Anxiety in Mice, Journal of Visual Experiments e52434, 1-6.
Semet, M., M. Paci, J. Saïas‐Magnan, C. Metzler‐Guillemain, R. Boissier, H. Lejeune, and J. Perrin, 2017, The impact of drugs on male fertility: a review, Andrology 5(4), 640-663.
Sestakova, N., A. Puzserova, M. Kluknavsky, and I. Bernatova, 2013, Determination of motor activity and anxiety-related behavior in rodents: methodological aspects and role of nitric oxide, Interdisciplinary Toxicology 6(3), 126-135.
Shearer, G.C., O.V. Savinova, and W.S. Harris, 2012, Fish oil—how does it reduce plasma triglycerides?, Biochimica et Biophysica Acta (BBA) 1821(5), 843-851.
Silva, M., L. Vieira, A.P. Almeida, and A. Kijjoa, 2013, The Marine Macroalgae of the Genus Ulva: Chemistry, Biological Activities and Potential Applications, Journal of Oceanography and Marine Research 1(1), 1-6.
Stanworth, R.D. and T.H. Jones, 2008, Testosterone for the aging male; current evidence and recommended practice, Clinical Interventions in Aging 3(1), 25-44.
Suroso A.S, 2013, Kualitas Minyak Goreng Habis Pakai Ditinjau dari Bilangan Peroksida, Bilangan Asam dan Kadar Air [Quality of Used Cooking Oil Viewed from Peroxide Value, Acid Value, and Moisture], Jurnal Kefarmasian Indonesia 3(2), 77-88.
Tomek, S.E. and M.F. Olive, 2018, Social Influences in Animal Models of Opiate Addiction. In International Review of Neurobiology 140, Academic Press, pp. 81-107.
Tseng, C.K. and C.F. Chang, 1984, Chinese seaweeds in herbal medicine, In: Eleventh International Seaweed Symposium (C.J. Bird & M.A. Ragan, Eds), Dr. W. Junk Publishers, Dordrecht, pp. 152-154.
DOI: https://doi.org/10.22146/jtbb.57924
Article Metrics
Abstract views : 4220
| 
views : 2321
Refbacks
- There are currently no refbacks.
Copyright (c) 2020 Journal of Tropical Biodiversity and Biotechnology
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Editoral address:
Faculty of Biology, UGM
Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia
ISSN: 2540-9581 (online)