* Corresponding Author
Morphological Characterization and Seed Germination Study of Wild Banana Musa acuminata var. flava (Ridl.) Nasution
https://doi.org/10.22146/jtbb.66645 Trimanto Trimanto(1), Elga Renjana(2), Dewi Ayu Lestari(3), Elok Rifqi Firdiana(4), Shofiyatul Mas’udah(5), Apriyono Rahadiantoro(6), Linda Wige Ningrum(7), Lia Hapsari(8*)
(1) Purwodadi Botanic Garden – Research Center for Plant Conservation and Botanic Gardens, National Research and Innovation Agency (BRIN), Jl. Surabaya – Malang Km 65, Purwodadi, Pasuruan, East Java, 67163, Indonesia
(2) Purwodadi Botanic Garden – Research Center for Plant Conservation and Botanic Gardens, National Research and Innovation Agency (BRIN), Jl. Surabaya – Malang Km 65, Purwodadi, Pasuruan, East Java, 67163, Indonesia
(3) Purwodadi Botanic Garden – Research Center for Plant Conservation and Botanic Gardens, National Research and Innovation Agency (BRIN), Jl. Surabaya – Malang Km 65, Purwodadi, Pasuruan, East Java, 67163, Indonesia
(4) Purwodadi Botanic Garden – Research Center for Plant Conservation and Botanic Gardens, National Research and Innovation Agency (BRIN), Jl. Surabaya – Malang Km 65, Purwodadi, Pasuruan, East Java, 67163, Indonesia
(5) Purwodadi Botanic Garden – Research Center for Plant Conservation and Botanic Gardens, National Research and Innovation Agency (BRIN), Jl. Surabaya – Malang Km 65, Purwodadi, Pasuruan, East Java, 67163, Indonesia
(6) Purwodadi Botanic Garden – Research Center for Plant Conservation and Botanic Gardens, National Research and Innovation Agency (BRIN), Jl. Surabaya – Malang Km 65, Purwodadi, Pasuruan, East Java, 67163, Indonesia
(7) Purwodadi Botanic Garden – Research Center for Plant Conservation and Botanic Gardens, National Research and Innovation Agency (BRIN), Jl. Surabaya – Malang Km 65, Purwodadi, Pasuruan, East Java, 67163, Indonesia
(8) Purwodadi Botanic Garden – Research Center for Plant Conservation and Botanic Gardens, National Research and Innovation Agency (BRIN), Jl. Surabaya – Malang Km 65, Purwodadi, Pasuruan, East Java, 67163, Indonesia
(*) Corresponding Author
Abstract
Wild bananas provide important genetic materials for further banana improvement, therefore they need to be conserved and studied. This study aimed to describe morphological characteristics of plant and seed and also to study the seed germination of wild banana M. acuminata var. flava (Ridl.) Nasution. The morphological characteristics were observed descriptively by referring to the descriptor for banana. The internal and external morphology of the seeds were observed using a digital microscope. The germination testing was carried out by a completely randomized design, using fresh seeds extracted from a bunch of fruits with two ripeness levels i.e. fully-ripe (yellow peel) and under-ripe (green-yellow peel). The data resulted was then analyzed using an independent t-test. The results showed that M. acuminata var. flava is characterized as a perennial herb; pseudostem height ≥3 m; male bud like a top with prominent green-yellow bracts; fruit curved and tasted mild-sweet when ripe. The seed is angular with wrinkled surface, and dark brown-black color when ripe. The longitudinal section showed parts of the seeds comprising the seed coat, outer and inner integument, embryo, endosperm, chalazal mass, micropyle cap and channel. The seeds are classified as orthodox, with hypogeal type and gradual germination pattern. The seeds extracted from fully-ripe fruit germinated faster with higher germination percentage and growth variables (root number and plant height). Thus, it is suggested to use physiologically mature seeds (seeds from fully-ripe fruits) which should be separated from the seeds of under-ripe fruits to lower the heterogeneity.
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Bohra, P., Waman, A.A. & Jerard, B.A., 2020. Seed germination and storage studies in seed-fertile Musa indandamanensis and its conservation. South African Journal of Botany, 128, pp.161–166. doi: https://doi.org/10.1016/j.sajb.2019.09.022.
Burgos-Hernández, M. et al., 2014. Seed germination of the wild banana Musa ornata (Musaceae). Seed Science and Technology, 42(1), pp.16–27. doi: 10.15258/sst.2014.42.1.02.
Chin, H.F., 1996. Germination and storage of banana seeds. New Frontiers in Resistance Breeding for Nematodes, Fusarium and Sigatoka. In EA Frison, JP Horry & D. De Waele, eds. Montpellier, France: INIBAP, pp. 218–227.
Darmayanti, A.S., Lestari, D.A. & Siahaan, F.A., 2017. Pengujian daya simpan dan viabilitas lima jenis biji tumbuhan langka: Parmentiera cereifera Seem., Santalum album L., Dillenia philippinensis Rolfe, Reutealis trisperma (Blanco) Airy Shaw dan Joannesia princeps Vell. Buletin Kebun Raya, 20(2), pp.65–78.
Dayarani, M. et al., 2014. Embryo culture and embryo rescue studies in wild Musa spp. (Musa ornata). Journal of Applied Horticulture, 16(2), pp.126–130. doi: 10.37855/jah.2014.v16i02.21.
Fidalgo, A. de O. et al., 2019. Pollination and quality of seeds and plantlets of Eugenia uniflora L. Hoehnea, 46(1), pp.1–12. doi: 10.1590/2236-8906-05/2018.
Ford-Lloyd, B. V. et al., 2011. Crop wild relatives - Undervalued, underutilized and under threat? BioScience, 61(7), pp.559–565. doi: 10.1525/bio.2011.61.7.10.
Hakkinen, M. & Vare, H., 2008. Typification and check-list of Musa L. names (Musaceae) with nomenclatural notes. Adansonia, 30(1), pp.63–112.
Hapsari, L., 2014. Wild Musa Species Collection of Purwodadi Botanic Garden: Inventory and Its Morpho-taxonomic Review. Journal of Tropical Life Science, 4(1), pp.70–81. doi: 10.11594/jtls.04.01.12.
Hapsari, L., Azrianingsih, R. & Arumingtyas, E.L., 2018. Genetic variability and relationship of banana cultivars (musa l.) from East Java, Indonesia based on the internal transcribed spacer region nrdna sequences. Journal of Tropical Biology and Conservation, 15(1), pp.101–120.
Hapsari, L., Lestari, D.A. & Probojati, R.T., 2020. Haplotype network analysis of wild banana relatives Ensete glaucum, Musa acuminata and Musa balbisiana based on cpdna rbcl sequences in ex-situ collection. Indian Journal of Genetics and Plant Breeding, 80(3), pp.301–307. doi: 10.31742/IJGPB.80.3.9.
Hastuti et al., 2019. Diversity wild banana species (Musa spp.) in Sulawesi, Indonesia. Biodiversitas, 20(3), pp.824–832. doi: 10.13057/biodiv/d200328.
Heslop-Harrison, J.S., 2011. Genomics, banana breeding and superdomestication. Acta Horticulturae, 897, pp.55–62. doi: 10.17660/ActaHortic.2011.897.4.
IPGRI-INIBAP, 1996. Descriptors for banana (Musa spp.), Maccarese: International Plant Genetic Resources Institute (IPGRI).
Kaiser, D.K. et al., 2016. Physiological maturity of seeds and colorimetry of the fruits of Allophylus edulis [(A. St.-Hil., A. Juss. & Cambess.) Hieron. ex Niederl.]. Journal of Seed Science, 38(2), pp.92–100. doi: 10.1590/2317-1545v38n2154590.
Kallow, S. et al., 2020. Challenges for ex situ conservation of wild bananas: Seeds collected in papua new guinea have variable levels of desiccation tolerance. Plants, 9(9), pp.1–21. doi: 10.3390/plants9091243.
Kallow, S. et al., 2021. Regulation of seed germination by diurnally alternating temperatures in disturbance-adapted banana crop wild relatives (Musa acuminata). Seed Science Research, 30(4), pp.238–248. doi: 10.1017/S0960258520000471.
Langhe, E. De et al., 2009. Why bananas matter: an introduction to the history of banana domestication. Ethnobotany Research and Applications, 7, pp.165–177.
Lestarini, W. et al., 2012. An alphabetical list of plant species cultivated in Purwodadi Botanic Garden, Pasuruan: Purwodadi Botanic Garden.
Martin, G. et al., 2020. Genome ancestry mosaics reveal multiple and cryptic contributors to cultivated banana. Plant Journal, 102(5), pp.1008–1025. doi: 10.1111/tpj.14683.
Moiwend, K.Y. et al., 2015. Uji viabilitas benih ketimun (Cucumis sativus L) hasil perlakuan penyerbukan berbagai serangga. Agrotekbis, 3(2), pp.178–186.
Muschick, M., Muschick, P. & Taylor, J., 2010. The evolution of seed testing. 29th ISTA Congress in Cologne, Germany, (139), pp.1–48.
Nasution, R.E., 1991. A taxonomic study of the species Musa acuminata Colla with its intraspecific taxa in Indonesia. Memoirs of Tokyo University of Agriculture, 32, pp.1–122.
Nasution, R.E. & Yamada, I., 2001. Wild bananas in Indonesia, Bogor: Pusat Penelitian dan Pengembangan Biologi LIPI.
Pedraza, T.R. et al., 2005. Production of banana and plantain hybrids in Cuba. InfoMusa, 14, pp.11–13.
Perrier, X. et al., 2009. Combining biological approaches to shed light on the evolution of edible bananas. Ethnobotany Research and Applications, 7, pp.199–216. doi: 10.17348/era.7.0.199-216.
Ploetz, R.C. et al., 2007. Species Profiles for Pacific Island Agroforestry Banana and plantain — an overview with emphasis on Pacific island cultivars. Http://Www.Traditionaltree.Org, (February).
Poerba, Y.S., Martanti, D. & Ahmad, F., 2019. Genetic variation of wild Musa acuminata Colla from Indonesia. Biotropia, 26(2), pp.115–126. doi: https://doi.org/10.11598/btb.2019.26.2.896.
Puteh, A.B. et al., 2011. Seed anatomy, moisture content and scarification influence on imbibition in wild banana (Musa acuminata Colla) ecotypes. African Journal of Biotechnology, 10(65), pp.14373–14379. doi: 10.5897/ajb11.1241.
Roostika, I. et al., 2019. Kultur embrio pisang liar Musa acuminata ssp. sumatrana yang langka. Jurnal Hortikultura, 28(1), pp.25–32. doi: 10.21082/jhort.v28n1.2018.p25-32.
Silva, M.D.S. et al., 2019. Illustrated guide to the classification of banana seeds and embryos. Revista Brasileira de Fruticultura, 41(2), pp.1–5. doi: 10.1590/0100-29452019089.
Singh, S. et al., 2021. Seed storage behavior of Musa balbisiana Colla, a wild progenitor of bananas and plantains – Implications for ex situ germplasm conservation. Scientia Horticulturae, 280, p.109926.
Sipen, P. et al., 2011. Genetic improvement of banana using conventional and In Vitro technologies. Journal of Crop Improvement, 25(6), pp.697–727. doi: 10.1080/15427528.2011.603406.
Sulistyaningsih, L.D., Megia, R. & Widjaja, E.A., 2014. Two New Records of Wild Bananas (Musa balbisiana and Musa itinerans) from Sulawesi. Makara Journal of Science, 18(1), pp.1–6. doi: 10.7454/mss.v18i1.3043.
Sutopo, 2010. Teknologi benih, Jakarta: PT. Raja Grafindo Persada.
Tillich, H.J., 2007. Seedling diversity and the homologies of seedling organs in the order Poales (monocotyledons). Annals of Botany, 100(7), pp.1413–1429. doi: 10.1093/aob/mcm238.
Valmayor, R.V. et al., 2002. Banana cultivar names and synonyms in southeast asia, Los Banos, Laguna, Philippines: IPGRI: Rome, Itali.
Villa, F. et al., 2019. Seed physiological quality and harvest point of dovyalis fruits. Pesquisa Agropecuaria Tropical, 49, pp.1–7. doi: 10.1590/1983-40632019v4954520.
Vineesh, P.S. et al., 2015. Seed germination and cryostorage of Musa acuminata subsp. burmannica from Western Ghats. South African Journal of Botany, 100, pp.158–163. doi: 10.1016/j.sajb.2015.05.024.
DOI: https://doi.org/10.22146/jtbb.66645
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