The Formula media in vitro Propagation and Conservation of Ludwigia sp.
Endang Gati Lestari(1*), Media Fitri Isma Nugraha(2), Rossa Yunita(3)
(1) Research Center for Horticulture and Estate Crops
(2) Research Center for Horticulture and Estate Crops
(3) Research Center for Horticulture and Estate Crops
(*) Corresponding Author
Abstract
The aquatic plant "Red Malang” (Ludwigia sp.) has a fairly high economic value as an ornamental aquatic plant, so it has the potential to be developed. The growth of in vitro cultures in culture bottles is high-speed, so it is necessary to find a formula media to inhibit growth so that the frequency of subcultures is reduced. The current research aims to produce a formula media for shoot multiplication and in vitro culture conservation. The research was carried out at the ICABIOGRAD tissue culture laboratory from April 2020 to June 2021. Research activities included plant propagation, conservation, and regeneration after conservation. Plant material was using in the form of a culture collection in the ICABIOGRAD tissue culture laboratory, treatment media for propagation were BA (0; 0.1; 0.3; 0.5; 0.7 and 0.9 mg/L) + thidiazuron (TDZ) (0 and 0.1mg/L). For conservation were MS + BA medium (0 and 0.1 mg/L) + paclobutrazol (0; 0.1; 0.3; 0.5; 0.7 mg/L) and for shoot regeneration after conservation using MS medium without Plant Growth Regulator (PGR). Data analysis using the Anova SAS version 9.0 test program. Further test using DMRT test with alpha level 5%. There was no difference in the mean value between levels of TDZ treatment on the number of shoots and leaves. The difference in the mean value between levels of TDZ treatment was very significant on shoot height, the number of roots, and root length. BA treatment with a concentration of 0.7 mg/L is better because it gives higher results for each observation variable. For conservation, treatment with paclobutrazol 0.5 mg/L inhibited shoot and leaf count, and 0.3 mg/L inhibited shoot formation. Cultures stored for six months grew normally after being regenerated. The highest shoots and the highest number of leaves were obtained from the treatment of paclobutrazol without BA. This study indicated that the propagation media of aquatic plants Ludwigia sp. did not require high concentrations of BA. Cultures could be stored for over six months using paclobutrazol with 0.3-0.6 mg/L.
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Abbas, M.S. et al., 2011. In Vitro Propagation of Ginger (Zingiber Officinale Rosc). Journal of Genetic Engineering and Biotechnology, 9(2), pp, 165–172. doi: 10.1016/j.jgeb.2011.11.002
Arrigoni-Blank, M. de F. et al., 2014. In Vitro Conservation of Sweet Potato Genotypes. The Scientific World Journal, 14, 208506. doi: 10.1155/2014/208506
Ashraf, M.F. et al., 2014. Effect of Cytokinin Types, Concentrations and Their Interactions on in Vitro Shoot Regeneration of Chlorophytum Borivilianum Sant. & Fernandez. Electronic Journal of Biotechnology, 17(6), pp.275–279. doi: 10.1016/j.ejbt.2014.08.004.
Bisht, T.S. et al., 2018. A Recent Advances in Use of Plant Growth Regulators (PGRs) in Fruit Crops - A Review. International Journal of Current Microbiology and Applied Sciences, 7(05), pp.1307–1336. doi: 10.20546/ijcmas.2018.705.159
Dewi, N., Dewi, I.S. & Roostika, I., 2016. Pemanfaatan Teknik Kultur In Vitro Untuk Konservasi Plasma Nutfah Ubi-Ubian. Jurnal AgroBiogen, 10(1), pp.34–44.
Hasan, M.A. et al., 2013. Paclobutrazol in Mitigating Irregular Bearing in Mango. Acta Horticulturae, 992, pp.173–180. doi: 10.17660/ActaHortic.2013.992.21
Huang, H.P. et al., 2014. Germplasm Conservation In Vitro of Polygonum Multiflorum Thunb. Pharmacognosy Magazine, 10(38), pp.179–184. doi: 10.4103/0973-1296.131032
Indrayanti, R. et al., 2018. Effect of Paclobutrazol for In Vitro Medium-Term Conservation of Banana Variant Cv. Kepok (Musa Acuminata x Balbisiana Colla). AIP Conference Proceedings, 020009. doi: 10.1063/1.5061845
Kumar, N., & Reddy. M.P., 2011. In Vitro Plant Propagation: A Review In Vitro Plant Propagation : A Review. Journal of Forest and Environmental Science, 27(2), pp.61–72. doi: 10.7747/JFS.2011.27.2.1
Lestari, E.G. & Hutami, S., 2005. Produksi bibit Kencur (Kaempferia Galanga L.) Melalui kultur Jaringan. Berita Biologi, 7, pp.315–321.
Lestari, E.G., 2011. Peranan Zat Pengatur Tumbuh Dalam Perbanyakan Tanaman Melalui Kultur Jaringan. Jurnal AgroBiogen, 7(1), pp.63–68.
Lestari E.G. et al., 2013. Inisiasi Tunas Ganda Tanaman Manggis Malinau melalui Kultur In Vitro untuk Perbanyakan Klonal. Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 41(1). doi: 10.24831/jai.v41i1.7075
Lestari, E.G., 2015. Peran Thidiazuron Dalam Peningkatan Kemampuan Proliferasi Tanaman Secara In Vitro. J. Litbang Pert., 34(2), pp.87–93.
Lestari, E.G. et al., 2021. Konservasi In Vitro Tanaman Hias Akuatik Bacopa Australis dan Alternanthera Reineckii Menggunakan Paklobutrazol Dan Benzil Adenin. Buletin Plasma Nutfah, 27(1), pp.11-20. doi: 10.21082/blpn.v27n1.2021.p11-20
M., Aziz A. et al., 2017. Induksi Tunas, Multiplikasi dan Perakaran Gyrinops versteegii (Gilg.) Domke Secara In Vitro. Jurnal Pemuliaan Tanaman Hutan, 11(1), pp.1–13. doi: 10.20886/jpth.2017.11.1.1-13
Mendes, M.I. de S. et al., 2021. In Vitro Conservation of Citrus Rootstocks Using Paclobutrazol and Analysis of Plant Viability and Genetic Stability. Scientia Horticulturae, 286, 110231. doi: 10.1016/j.scienta.2021.110231
Mog, B. et al., 2019. Manipulation of Vegetative Growth and Improvement of Yield Potential of Cashew (Anacardium Occidentale L.) by Paclobutrazol. Scientia Horticulturae, 257, 108748. doi: 10.1016/j.scienta.2019.108748
Muengkaew, R. & Chaiprasart. P., 2016. Effect of Paclobutrazol Soil Drenching on Flowering of ‘Mahachanok’ Cultivar. Acta Horticulturae 1111, pp.323–328. doi: 10.17660/ActaHortic.2016.1111.46
Murashige, T. & Skoog, F., 1962. A revised for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15, pp.473-497.
Negi, S.S., Lal, S. & Sah, H., 2017. Influence of Growth Retardant on Vegetative Growth and Leaf Chlorophyll Content of Jackfruit (Artocarpus Heterophyllus).Int. J. Pure App. Biosci., 5(1), pp.142-146. doi: 10.18782/2320-7051.2466
Nugraha, M.F.I. et al., 2017. Pembentukan Mother Plant Bacopa Australis Secara In-Vitro dan Aklimatisasi Dalam Aquascape Air Tawar. Media Akuakultur, 12(2), pp.85-94. doi: 10.15578/ma.12.2.2017.85-94
Nugraha, M.F.I. et al., 2018. Tanaman Hias Air Tawar Manfaat Untuk Perairan Perbanyakan Konvensional Dan in vitro, Jakarta, ID: AMAFRAD Press.
Rahayu, E.S., Habibah, N.A. & Herlina, L., 2015. Development of In Vitro Conservation Medium of Carica pubescens Lenne & K. Koch through Nutrients Concentration Reduction and Osmoregulator Addition. BIOSANTIFICA Journal of Biology and Biology Education, 7(1), pp.22-28. doi: 10.15294/biosaintifika.v7i1.3538
Roostika, I., Purnamaningsih, R. & Darwati, I., 2009. Penyimpanan In Vitro Tanaman Purwoceng (Pimpinella Pruatjan Molk.) Melalui Aplikasi Pengenceran Media Dan Paclobutrazol. Jurnal Littri, 15(2), pp.84–90.
Satyavathi, V.V. et al., 2004. Effects of Growth Regulators on in vitro Plant Regeneration in Durum Wheat. Crop Science, 44(5), pp.1839–1846. doi: 10.2135/cropsci2004.1839
Siddique, I. et al., 2015. Influence of Plant Growth Regulators on in Vitro Shoot Multiplication and Plantlet Formation in Cassia Angustifolia Vahl. Brazilian Archives of Biology and Technology, 58(5), 686–691. doi: 10.1590/S1516-89132015050290
Silva, T. dos S. et al. 2019. In Vitro Conservation of (Poincianella Pyramidalis (Tul.) L.P. Queiroz under Minimal Growth Conditions. Ciência e Agrotecnologia, 43, e014519. doi: 10.1590/1413-7054201943014519
Supriati, Y., Mariska, I. & Hutami, S., 2005. Mikropropagasi Sukun (Artocarpus Communis Forst ), Tanaman Sumber Karbohidrat Alternatif. Berita Biologi, 7(4), pp.207–214. doi: 10.14203/beritabiologi.v7i4.1049
Syahid, S.F. & Kristina, N.N., 2008. Multiplikasi Tunas, Aklimatisasi Dan Analisis Mutu Simplisia Daun Encok (Plumbago Zeylanica L.) Asal Kultur In Vitro Periode Panjang. J Bul Littro. J Buletin Penelitian Tanaman Rempah dan Obat, 19(2), pp.117–128.
Yunita, R. et al., 2018. Perbanyakan Tanaman Hias Air Bacopa Australis Secara In Vitro Pada Berbagai Formulasi Hormon Media Pertumbuhan. Media Akuakultur, 13(2), pp.75-82. doi: 10.15578/ma.13.2.2018.75-82
DOI: https://doi.org/10.22146/jtbb.75947
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